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Description
Human loxl1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Lysyl Oxidase-Like Protein 1 (LOXL1). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Lysyl Oxidase-Like Protein 1 (LOXL1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Lysyl Oxidase Like Protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Lysyl oxidase homolog 1, also known as LOXL1, is an enzyme encoded by the LOXL1 gene. This gene encodes a member of the lysine oxidase gene family. The prototypical member of this family is essential for connective tissue biogenesis, encoding an extracellular copper-dependent amine oxidase that catalyzes the first step in the formation of collagen and elastin cross-links. A highly conserved amino acid sequence at the C-terminus appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is less conserved, potentially conferring additional roles to each family member in developmental regulation, aging, tumor suppression, cell growth control, and chemotaxis. Polymorphisms in the LOXL1 gene have been associated with pseudoalopecia syndrome, a disorder in which the extracellular matrix contains abnormal amounts of cross-linked, amyloid-like fibrils and glycoproteins. When this occurs in the eye, it results in exfoliation glaucoma. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.9 ★★★★★
Based on 9 reviews
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Product Reviews
★★★★★ 5
Easy Install, Great Quality, Mobile Partition
Color: Grey
This is the easiest install I've ever had in my life, took about 5 minutes. These panels come pre-assembled, minus the wheels, which screw into the base pieces in pairs. The material is a very pleasant "corporate grey" - I only worry about its color fastness over time.
I needed a practical solution for my garage, which has glass garage doors that let in a ton of sun/heat and even with privacy film still parade around my tools and belongings visibly to anyone walking by. These partitions are functional for both of my needs, and if I have a visitor needing to park in my second spot it takes 5 seconds to wheel these out of the way.
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Reviewed in the United States on March 15, 2026
★★★★★ 5
Nice, convenient partition that absorbs sound
Color: Grey, Color: Grey
What stood out
* Instant partition: This 3-panel foldable office partition helps to create quiet zones in a shared room, and it genuinely helped carve out a more focused space without a major renovation. The panels feel substantial yet easy to move thanks to the wheels, so I can roll them into position when I need privacy or a professional background for video meetings. I can fold them flat against a wall when I don’t nee them. The aluminum frame gives it enough rigidity to stay upright, and the gray finish is neutral. The height and width are generous for desktop setups, meetings, or study areas, and because it’s modular you can arrange it in L-shapes, straight lines, or even semi-circles. The mobility makes it flexible for dynamic spaces — roll it out for working hours, tuck it away after.
* Sound insulation: It's heavy, which is better for sound isolation. The sound-dampening design isn’t miracle acoustic treatment, but it noticeably softens background chatter and echoes — enough to make video calls and focused work feel less disrupted.
* Assembly is super easy, but I would recommend to have two people to make it easier.
What to keep in mind
* It’s not sound-proof like a built-in wall, so loud noises still carry; it’s best for softening ambient sounds, not blocking them completely.
* The gray color is neutral, but if your room palette is bold you might want to accent with décor so it doesn’t look utilitarian. You can also tack posters and light pictures to the the gray frames.
* It's pricey, but worth it in my opinion.
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Reviewed in the United States on January 19, 2026
★★★★★ 4
Decent dividers
Color: Grey
I think you will love these under the right circumstances. Number one the sound absorption is hard to describe because since the panels don't go to the roof sound can easily travel to the other so you and without being hindering but they do absorb sound to a certain extent.
They also provide pretty good coverage for privacy in an office or dorm situation with ease. I wouldn't say it's easy to store but it does fold up pretty well and overall for the price it's an effective means of letting those around you know you need some peace.
For the price it's a pretty good divider. It requires very little assembly and is relatively sturdy so all in all not a bad option.
Until We Meet Again
-Off The Grid Ken
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Reviewed in the United States on March 8, 2026
★★★★★ 5
Very functional, well made, and looks great!
Color: Grey
My wife and I both work from home but for different companies. We have separate office spaces but they are without a door between them. There are times when we both are on either voice or video calls when we can hear each other on the calls. Other times, I have to walk past her office space and behind her desk to get to other areas of the house. We really needed something to help block some of the ambient sound and to obscure me from her video calls when I'm going past to let the dogs out. These foldable partitions are a great solution for us. The wheels make them easy to move into place when needed, and to store away when they are not. The wheels also lock, making them stable where we set them. The assembly was easy and quick, with only the simplest of instructions needed. Assembly tools were even included. These are large and a bit heavy for one person to move before assembly, so get the wheels on them for easier movement. I've researched how much these can cost new from the commercial office suppliers, and these are a good value.
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Reviewed in the United States on January 23, 2026
★★★★★ 5
Instant Privacy With a Professional Look
Color: Grey
I needed a flexible solution for a shared office space, and this foldable partition worked better than expected. Setup was straightforward, and the wheels make it easy to move around without scratching the floor. It noticeably reduces noise and distractions, which helped my focus more than I anticipated. The gray color and aluminum frame look clean and professional, not temporary or flimsy. It’s not fully soundproof, but it definitely softens background noise. Storage is easy since it folds up neatly when not in use. For home or office use, it’s practical and well designed.
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Reviewed in the United States on February 2, 2026