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Description
Human Bruton'S Tyrosine Kinase ELISA KitProduct Specification Usage Sample collection, processing and storage methods: 1, serum: use do not contain pyrogen and endotoxin in vitro, in the process of operation to avoid any cell stimulation, collect blood after 3000 turn centrifugal 10 minutes to separate serum and erythrocyte quickly carefully. 2, plasma: EDTA, citric acid salt or heparin anticoagulation. The supernatant was removed by centrifugation at 3000 RPM for 30 min. 3, on the cell
Product Specification
| Usage | Sample collection, processing and storage methods: 1, serum: use do not contain pyrogen and endotoxin in vitro, in the process of operation to avoid any cell stimulation, collect blood after 3000 turn centrifugal 10 minutes to separate serum and erythrocyte quickly carefully. 2, plasma: EDTA, citric acid salt or heparin anticoagulation. The supernatant was removed by centrifugation at 3000 RPM for 30 min. 3, on the cell supernatant: 3000 turn 10 minutes of centrifugal to get rid of particle and polymer. 4. Tissue homogenate: add appropriate amount of normal saline to the tissue and mash it. 3000 turn centrifugal take supernatant 10 minutes. 5. Storage: if the sample is not detected in time after collection, please pack according to the dosage, freeze at -20℃, avoid repeated freezing and thawing, thaw at room temperature and ensure that the sample is evenly and fully thawed. Bring your own items: 1. Microplate reader (450nm) 2 washer, high precision and spear: 0.5-10 ul, 2-20 ul, 20-200 - ul, ul 200-1000 3, 37℃ incubator The reagent preparation: Dilution of 20× wash buffer: Distilled water was diluted 1:20, that is, 1 part of 20× wash buffer was added to 19 parts of distilled water. Washing plate method: 1, hand washing board: jilt liquid in the hole, every hole filled with liquid detergent. Let stand for 1 min after the left hole all liquid, pat dry on blotting paper, so wash board 5 times. 2. Automatic washing machine: inject 350μL of washing solution into each well, soak for 1min, and wash the plate 5 times. Operation steps: 1. Remove the required slats from the aluminum foil bag after 20min of equilibrium at room temperature, and the remaining slats are sealed with a ziplack and placed back at 4 ° C. Bore 2, set up the standard and sample, standard holes with different concentrations of standard 50 mu L; 3. Add 10μL sample to the sample well, and then add 40μL sample diluent; Blank holes are not added. 4, in addition to the blank hole, bore standard and sample of each hole to join horseradish peroxidase (HRP) labeled 100 mu L detection antibody, seal plate film sealed reaction with hole, 37 ℃ water bath pot or constant temperature box temperature 60 min. 5, abandon to liquid, pat dry on the blotting paper, every hole filled with liquid detergent. Let stand for 1 min, jilt to detergents, pat dry on the blotting paper, repeat 5 times wash plate (also available microplate wash plate). 6, 50μL of substrate A and B were added to each well and incubated at 37 ° C in the dark for 15min. 7, 50 mu, L per hole to join terminated liquid within 15 min, determine the hole in the 450 - nm wavelength, OD value. Results: Drawing standard curve: in Excel worksheets, with standard concentration as the abscissa, ordinate corresponding OD value, draw the standard linear regression curve, the curve equation of the sample density is calculated. |
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| Theory | Double-antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA) was used. Samples, standards, and HRP-labeled detection antibodies were added to the coated microwells precoated with Bruton's tyrosine kinase (Btk) antibody in turn, then incubated and washed thoroughly. After incubation and thorough washing, the substrate TMB was used for color development. TMB was converted to blue under the catalysis of peroxidase and to yellow under the action of acid. The degree of color is positively correlated with the level of Bruton's tyrosine kinase (Btk) in the sample. The activity of the samples was calculated by measuring the absorbance (OD) value at 450nm using a microplate reader. | |||||||||||||||||||||||||||||||||
| Detection Type | Serum, cell supernatant, and tissue homogenate urine | |||||||||||||||||||||||||||||||||
| Description |
Bruton's tyrosine kinase (Btk or BTK for short), also known as tyrosine protein kinase BTK, is a tyrosine kinase encoded by the BTK gene. BTK plays a crucial role in B cell development. BTK contains five different protein interaction domains. These domains include an amino-terminal pleckstrin homology (PH) domain, a proline-rich TEC homology (TH) domain, the SRC homology (SH) domains SH2 and SH3, and a kinase structure with enzymatic activity. BTK plays a critical role in B cell development because it is required to transmit signals from pre-B cell receptors formed after successful immunoglobulin heavy chain rearrangement. Btk contains a PH domain that binds to phosphatidylinositol-triphosphate (PIP3). Binding of PIP3 induces Btk to phosphorylate phospholipase C, which in turn hydrolyses PIP2 (a phosphatidylinositol) to two second messengers, inositol triphosphate (IP3) and diacylglycerol (DAG), which then regulate the activity of downstream proteins during B-cell signaling. kit performance: |
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| Composition |
Note: The concentrations of standard substance (S0-S5) were 0, 75, 150, 300, 600, 1200 U/mL |
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| General Notes | 1. The kit was stored at 2-8 ° C and equilibrated at room temperature for 20 minutes before use. The concentrated washing solution removed from the refrigerator will have crystallization, which is a normal phenomenon, and the water bath will be heated to completely dissolve the crystals before use. 2. The slats that are not used in the experiment should be put back into the ziplock bag immediately, sealed (dry at low temperature) and stored. 3, concentration of 0 S0 standard can be regarded as negative control or blank; When operating according to the instructions, the sample has been diluted 5 times, and the final result multiplied by 5 is the actual concentration of the sample. 4, in strict accordance with the instructions indicated in the time, the amount of liquid and the order of the incubation operation. 5, all liquid components shake well before use. |
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| Storage Temp. | 2-8℃, protected from light and moisture, valid for 6 months. |
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| Test Range | 0.625-20 ng/mL |
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4.8 ★★★★★
Based on 22 reviews
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Product Reviews
★★★★★ 5
Exactly as described
Size: Crescent Queen
This pillow cover is everything they said it would be. Comfortable, no crinkle sound. Fits the pillow like a glove. I highly recommend.
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Reviewed in the United States on April 26, 2026
★★★★★ 5
Great for side sleepers
It always seems hard to find the perfect pillow when you’re a side-sleeper. This one is great. It comes compressed so you will want to allow some time for it to fully fluff back up. The cover is soft and smooth. I love that it isn’t overstuffed. It provides gentle support and a lot of comfort. I was worried it may be too warm since it is polyester, but it is very breathable and I have not had any issues with feeling overly warm on it. It fits a standard size pillow case. It’s not overly stuffed like some other pillows I’ve tried that were specifically for side-sleepers. I think this world be a great pillow for any sleeping position, really. I wasn’t expecting to love a pillow this affordable, but it gets the job done and I get a good night of sleep. I’ll be purchasing some more for our guest room.
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Reviewed in the United States on May 10, 2025
★★★★★ 5
Incredibly Comfortable and Supportive — My New Favorite Pillow！
I wasn’t expecting to love this pillow as much as I do, but the DIORIS large sleeping pillow really exceeded my expectations. From the first night, I noticed a difference in the way my neck and shoulders felt in the morning—less tension, better alignment, and overall more restful sleep.
The fill is just the right balance between plush and supportive. It doesn’t flatten out during the night, which is a problem I’ve had with other pillows, especially those that come overstuffed or too soft. This one holds its shape well but still has that cozy, cloud-like feel when you lay your head on it.
I’m a side and back sleeper, and this pillow works beautifully in both positions. It’s also large enough that I don’t feel like I’m falling off it during the night, but not so big that it takes over the whole bed.
One suggestion: if you’re picky about loft, you might want to give it a gentle fluff or adjust the fill before your first night. But honestly, I didn’t need to do much—it was pretty much perfect out of the bag.
Highly recommended if you're looking for a high-quality, comfortable, and generously sized pillow that supports a great night’s sleep!
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Reviewed in the United States on May 9, 2025
★★★★★ 5
Hotel like pillow
These pillows were a pleasant surprise. I bought the set for our guest room but ended up keeping them for our own bed after trying them out. They feel incredibly soft yet still provide enough support to keep my neck from feeling stiff in the morning. I sleep on my side and back, and these work great for both positions. My spouse, who usually runs hot at night, said they stayed cool and breathable.
The material feels high quality, and I love that they’re machine washable without losing their shape. They fluffed right back up after drying. If you're looking for that plush hotel-pillow feel with everyday practicality, this set really delivers.
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Reviewed in the United States on May 15, 2025
★★★★★ 4
Very soft, not firm at all
This pillow is very floppy and not firm at all. It is quite large and I was surprised at the size. My dog has taken a liking to it and decided it's now her new dig bed, which is fine by me, because it's really too soft for my liking. If you like soft pillows then this is for you. If you require something with a bit more support then you won't enjoy this pillow.
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Reviewed in the United States on May 11, 2025