SKU: 63137015094

UA-Blue SEAP Detection Kit

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Description

UA-Blue SEAP Detection KitProduct Specification Synonyms Alkaline phosphatase detection reagent Stability & Storage Store reagents at 20C or below, protected from light . Shelf Life: 12 months when stored as recommended. For research use only. Background Secreted Alkaline Phosphatase (SEAP) is widely used in reporter gene analysis studies. Its advantage is that it only requires the analysis of cell culture supernatant, without the need to lyse cells. Moreover, SEAP is

Product Specification


Synonyms Alkaline phosphatase detection reagent
Stability & Storage

Store reagents at -20°or below, protected from light . Shelf Life: 12 months when stored as recommended. For research use only.

Background

Secreted Alkaline Phosphatase (SEAP) is widely used in reporter gene analysis studies. Its advantage is that it only requires the analysis of cell culture supernatant, without the need to lyse cells. Moreover, SEAP is relatively heat-resistant and has a high tolerance for specific alkaline phosphatase inhibitors. Therefore, samples can be subjected to specific pre-treatment to inactivate endogenous alkaline phosphatase and eliminate interference.

The UA-Blue SEAP Detection Kit is a homogeneous ready-for-use reagent that completes the detection through the simple steps of “add-mix-detect.” This kit is used to detect alkaline phosphatase activity in the culture medium of cells transfected with the SEAP reporter gene. There is no need to lyse the cells, which can be further cultured or used for other multiplex analyses. Compared with other similar products, the detection sensitivity is at least one time higher, and the reaction time is shortened by at least one time. It is particularly suitable for the analysis of cells with low enzyme activity and the screening of inhibitory compounds.

Components

Size

Buffer (40x)

Substrate (250x)

Tests in 96-well plates

Tests in 384-well plates

2500 tests

5x 2.5 ml

5x 400 ml

2,500

12,500

25000 tests

5x 25 ml

5 x 4ml

25,000

125,000


Protocol

1). Cell Seeding & Preparation

Plate cells expressing SEAP reporter gene in 96-well or 384-well plates.

If using FBS-containing medium: Heat-inactivate FBS at 56°C for 30 min to eliminate endogenous alkaline phosphatase (AP) activity.

 

2). Cell Treatment & Stimulation

Treat cells with test compounds and stimulate reporter gene expression as required.

 

3). Sample Collection & Pre-Treatment

Collect cell culture supernatant at the desired timepoint post-stimulation. For high endogenous AP background: Heat samples at 65°C for 5-10 min to inactivate interfering phosphatases (optional).

Note: The QT-Blue™ SEAP Assay Kit reagent suppresses low levels of endogenous AP without affecting SEAP activity.

 

4). Reagent Preparation

Equilibrate UA-Blue detection reagent to room temperature (RT) before use.

 

Buffer Handling (40×  Buffer)

May appear slightly turbid (no precipitate) - vortex well before dilution.

Storage: 4°C: Stable for 2 weeks; -20°C: Long-term storage

 

Substrate Handling (250× Substrate)

Appearance: Red-brown solution; precipitate may form during storage.

To resolubilize: Vortex vigorously for 1–2 min, OR Incubate at 37°C for 5–10 min.

Storage: 4°C: Stable for 4 weeks; -20°C: Long-term storage

 

5). 1× Working Solution Preparation

Example (40 mL 1× detection reagent):

- Dilute 1 mL 40× Buffer in 39 mL sterile water → 1× QB Buffer.

- Add 167 µL 250× Substrate → immediate flocculation occurs. Vortex immediately until fully dissolved (clear solution). Delay in mixing causes irreversible precipitation.

 

6). HTS Optimization (e.g., 1536-well plates):

Prepare 3× or 6× concentrated detection reagent to minimize pipetting steps. Example:

3× reagent (2 µL) + 4 µL sample → 1× final concentration.

 

Storage for 1x/3x/6x detection reagents: 4°C, stable for 1 week (may form slight precipitate; vortex before use).

 

7). Assay Setup (96-Well Plate Example)

- Add 20 µL sample/control per well.

- Add 180 µL 1× detection reagent.

- Mix on plate shaker (15 sec).

 

8). Incubation: 37°C for 30 min-6 hrs (protect from light).

 

9). Detection: Measure absorbance at 600-650 nm (OD) using a plate reader.

Guidelines

Signal Stability: Readings should be taken within 6 hr of reagent addition.

HTS Adaptation: For 384/1536-well plates, scale down volumes proportionally.

Interference Control: Heat pretreatment (65°C) is recommended for high-background samples.

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SKU: 63137015094

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Estela fransua
Alexandria, US
★★★★★ 5
Useful Addition to My Kitchen
Size: Paper Towel Holder - Standard Design, Size: Paper Towel Holder - Standard Design
This has been a useful addition to my kitchen. It is simple, convenient, and works well for my daily routine. I would recommend it.
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Reviewed in the United States on May 31, 2026
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dooders
Battle Creek, US
★★★★★ 5
Paper towel holder with easy spray
Size: Paper Towel Holder - Standard Design
It’s handy to have paper towels and cleaning spray together in one unit
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Reviewed in the United States on April 12, 2026
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seashell
Houston, US
★★★★★ 5
Best paper towel holder
Color: Silver
Very easy to put together, very sturdy and works great! Holds the paper towel with no slipping or popping off and it does not move around on the counter because of the suction cups. Very satisfied with the purchase. Bought another for the bathroom and one for my mom.
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Reviewed in the United States on March 4, 2026
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Janis
Belleville, US
★★★★★ 5
Perfection!
Color: White, Color: White
Oh my! A design so simple but so effective! I had a towel holder but when I went to pull a piece off..it would hardly budge. I use jumbo towels(triple rolls from Costco)I would have to hold it. And it would only work smoothly when the roll was down some. Then it would spin too fast!🙄 This holder..nope..the suction cups are nice and strong..and when I pulled a piece off..the spin was perfectly smooth and calculated... and you can tear off the piece perfectly You can hear the spin movement. SOOO glad I bought this..yes..it is expensive for a towel holder BUT...it does EVERYTHING its supposed to do! In my book...worth it. Do not hesitate...it's a keeper! And the white is perfect for my bathroom...blends in beautifully. But the choices are nice for any decor.
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Reviewed in the United States on May 16, 2025
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toots
San Leandro, US
★★★★★ 5
Excellent for one-handed operation
Color: Silver
This one-handed paper towel dispenser is very good quality. The suctions cups work very well on a clean, smooth surface. Has a good solid feeling while pulling off paper towels with one hand. You might have a slight learning curve on to how to pull correctly. Pull the paper towel only just far enough out that there is still good tension on the towel. Then pull the towel downward and close to the roll. A little practice will be rewarded with satisfaction of this product! Comes in so handy when you have one messy hand and only your other hand to pull off one or several towels. If pulling several sheets at once remember to pull them straight outward, then pull the last sheet downward and close to the roll. No fuss removing the used cardboard roll and replacing with a new roll. Assembly is easy. The assembly instructions aren't good quality but the parts only fit together one way. The dispenser will end up looking like a two-tier spoon carousel with the spoons hanging upside down. I would buy this as a gift for family or friends.
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Reviewed in the United States on May 6, 2024

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