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Description
Rat Kim-1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Urine Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH=7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Urine Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH=7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes and remove the supernatant for analysis. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, but avoid repeated freezing and thawing. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Sensitivity | 0.075 ng/mL | |||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Kidney Injury Molecule 1 (Kim-1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Kidney Injury Molecule 1 (Kim-1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Kidney Injury Molecule 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Kidney injury molecule 1 (Kim1), also known as hepatitis A virus cellular receptor 1 (HAVcr-1) or T cell immunoglobulin and mucin domain 1 (TIM-1), is a protein encoded by the HAVCR1 gene. It is a member of the TIM (T cell transmembrane, immunoglobulin, and mucin) gene family and plays a key role in regulating immune cell activity, particularly the host response to viral infection. It is also involved in allergic reactions, asthma, and transplant tolerance. The TIM gene is a type I cell surface glycoprotein composed of an N-terminal immunoglobulin (Ig)-like domain, a significant mucin domain, a transmembrane domain, and a short C-terminal cytoplasmic tail. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Urine, tissue homogenates, cell culture supernatants, and other biological fluids |
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4.1 ★★★★★
Based on 9 reviews
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Product Reviews
★★★★★ 4
Too baggy! (UPDATE): Bought this style of jeans again and they fit great!
Size: 31W x 30L, Color: Tinted Light Wash
These jeans are too baggy for my liking.
Edit: I purchased more jeans of this style recently again and for some reason they fit a lot better to my liking than before when I first left the initial review for this.
I have updated my review from 3 stars to 4 stars now. I purchased the black, grey, dark wash and tinted medium blue, and all 4 had a good fit on me.
My original review stated that it was too baggy but the 4 jeans I just bought had a fitted look which is what i prefer, not too baggy and not to slim/skinny.
I want to point out that the fit varies from the color choices. For example, the black, grey and dark wash jeans, i went with a size 32x30 because 31x30 for those colors were too tight/slim on me and for the tinted medium blue, i downsize to 31x30 because 32x30 for this color is too baggy.
So buy the size you usually wear and if it's too slim or baggy then send it back and size up or down. Buying jeans on Amazon, i feel that you will have to buy a few different sizes and see which one fits you the best and then go from there.
Edit (3/7/26): sizing is very inconsistent. So my previous edit above stated that the jeans I recently purchased fit great on me, and that's true, so I decided to buy another jean, same color and same size, this time the jean fit way too small/tight. I feel like amazon needs to do better quality control on these jeans. How does 2 pairs of the same jeans/color/size have such an inconsistent in size/fitting to it!
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Reviewed in the United States on December 14, 2025
★★★★★ 5
Great fitting jeans just as nice as any name brand you can buy !
Size: 34W x 28L, Color: Tinted Light Wash
So I have been on a weight loss journey and the Amazon jeans have been my go to. For one to me they are just as nice as any of the name brands. They fit well are comfortable and honestly they are all I have purchased for a few years now. I’m also super short and this is one of the few brands I can find in a 29 length and that also saves me money since I don’t have to take them to a tailor to be modified. Material is nice to the touch nice thickness and has all the pockets you would expect in a blue jeans. Again great value for the money and this is about my 5th pair of these in various colors. I highly recommend give them a try and you will be hooked !
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Reviewed in the United States on December 14, 2025
★★★★★ 5
Definitely a repurchase!
Size: 31W x 30L, Color: Tinted Light Wash
Love these jeans! Fit so well and feel great too
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Reviewed in the United States on April 16, 2026
★★★★★ 5
Nice jeans for the price.
Size: 35W x 34L, Color: Black-heavyweight
Very comfortable and well fitting pants. The only issue I have is it's not tapered towards the bottom of the leg and doesn't look as fitted but overall is a very good pair of pants for the price.
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Reviewed in the United States on May 7, 2026
★★★★★ 1
Cheap
Size: 34W x 32L, Color: Stone Medium Wash
The fit is pretty terrible. Bunchy in the crotch. Lack of inseam lengths so they can be either too long or too short depending on your height. I would have rated these a 3 overall because they are what you pay for. Passable cheap jeans, but honestly you're better off shopping cheap department store jeans. I give it a one for durability. Only a handful of wears since September and they've torn at the stitching under the zipper just after a very light lunge. Buyer beware .
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Reviewed in the United States on February 6, 2026