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Description
Human LOX ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a lysyl oxidase (LOX) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of lysyl oxidase (LOX) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Lysyl Oxidase ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Lysine oxidase (LOX), also known as protein lysine 6-oxidase, is encoded by the LOX gene. It catalyzes the conversion of lysine molecules into highly reactive aldehydes, which form cross-links in extracellular matrix proteins. Its inhibition can cause osteoporosis, but its upregulation by tumor cells can also promote metastasis, leading to malignant and cancerous transformation of existing tumors. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.6 ★★★★★
Based on 17 reviews
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Product Reviews
★★★★★ 4
Nice office chair!
Color: Dark
This is a nice office chair. All of the features work as stated. I would say the seat is slightly more firm than I prefer, but comfort is subjective. The pleather is soft and I like that the arm rests fold out of the way.
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Reviewed in the United States on May 11, 2026
★★★★★ 5
This Marsail Executive Office Chair Is Excellent For A Comfortable And Polished Desk Setup
Color: Dark
This Marsail executive office chair is excellent, and it brings together comfort, support and a clean professional look in a very practical way. The PU leather has a smooth, polished presence that suits a desk chair well without looking overly bulky. I appreciate that the design includes flip-up armrests, a height-adjustable seat, swivel movement and rolling mobility in one complete chair. The cushioning gives the chair a substantial seated feel, while the back design adds support without making the chair look heavy. It is an office chair that feels thoughtfully built for daily desk work, focused tasks and general seated comfort.
The chair arrived securely protected and in very good condition, with the main parts kept in place well through transit. The PU leather surfaces looked clean, smooth and free from distracting marks when unpacked. The base, wheels, armrests, backrest and hardware all appeared properly finished and ready for assembly. Nothing about the chair parts looked loose, flimsy or poorly handled when it arrived. The included components were organized.
The appearance of this chair is one of its strongest qualities because it has a dark, sleek and professional style. The chair fabric gives it a refined executive look, while the breathable mesh center keeps the backrest from appearing too solid or oversized. The flip-up armrests blend into the design nicely and add a cleaner profile when raised. The chair has a balanced shape, with a supportive back, plush seat and wide base that all work together visually. It looks modern without being too flashy, which makes it easy to place with different desks and work areas.
Functionality is where this chair becomes especially practical because it is easy to put together and simple to adjust. The height adjustment works well for getting the seat into a comfortable position at a desk. The swivel and rolling movement make it easy to shift, turn and reach without the chair feeling stiff. The reclining range and gentle rocking function add a useful option for leaning back during breaks. The flip-up armrests are especially convenient because they allow the chair to tuck under a desk more easily and create extra freedom of movement when needed.
The value is strong because this chair offers several useful features in one well-rounded design. The thicker cushioning, contoured support, PU leather surface, breathable backrest, reclining function and flip-up armrests all add to its usefulness. The sturdy base and stated 300-pound support rating also give it a more dependable feel than a basic desk chair. It provides the comfort expected from an executive chair while still offering space-saving details that make it practical. For the features, comfort and overall build, it delivers a very complete office seating option.
I highly recommend this Marsail executive office chair for anyone wanting a comfortable, polished and functional desk chair. It is excellent, easy to put together and built with enough adjustability to support different work habits. The combination of plush cushioning, ergonomic shaping, smooth movement and flip-up armrests makes it especially useful for a home office or workspace. I also like that it has a professional look without sacrificing everyday convenience. It is a strong choice for dependable seating that looks good, works well and offers solid comfort.
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Reviewed in the United States on May 5, 2026
★★★★★ 5
Comfortable for daily use!
Color: Dark, Color: Dark
After using this chair for work and general desk time, I think it does a nice job balancing comfort, adjustability, and overall build quality without feeling overly bulky or cheap. It feels designed for people who spend long stretches at a desk and want something supportive but still comfortable enough to relax in occasionally.
Assembly was straightforward. The instructions were clear, the hardware was organized well, and all the holes lined up properly during setup. I put it together by myself in around 20 minutes without needing extra tools. Once assembled, the chair immediately felt sturdy and stable rather than wobbly.
The overall quality is solid for this price range. The PU leather has a soft feel and doesn’t have the stiff plastic-like texture some office chairs use. The stitching looks clean, the armrests feel secure, and the rolling base moves smoothly across the floor. The chair also feels durable overall, especially around the frame and base where cheaper chairs sometimes feel weak or shaky after a few weeks of use.
It's very comfortable. The seat cushion is thick and supportive without feeling overly hard, and it stays comfortable during long work sessions. I’ve spent multiple hours at a time sitting in it without constantly adjusting positions or feeling pressure points. The mesh section in the backrest also helps airflow a bit, which keeps it from feeling too hot during extended use.
The lumbar support is great. The backrest naturally follows the curve of your spine and provides good lower back support while still feeling relaxed enough for casual sitting. I also liked the higher back design since it supports the upper back and shoulders well during longer periods at the desk.
The flip-up armrests ended up being more useful than I expected. They make it easy to slide the chair under the desk when not in use, and they also give more room if you like sitting cross-legged occasionally.
Solid chair overally. It has a good mix of comfort, support, ease of use, and practical features without feeling overpriced.
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Reviewed in the United States on May 26, 2026
★★★★★ 4
Comfortable, easy to assemble, but no adjustable armrests
Color: Dark, Color: Dark
The Marsail Executive Office Chair Ergonomic Desk Chair with Flip-up Armrests PU Leather was an easy to assemble chair; I was done within 15 minutes with the included tool. Extra screws were included which was nice as the main pack had broken open and I couldn't find one of the primary screws in the larger box (my guess is I probably tossed it on accident with the packaging material) but had the backup so all was well. The instructions were well written and clear.
I've now had the chair in my office for over a week. For context; I work from home and also am a PC gamer so I spend a sizeable portion of my time at my desk in the chair. I also live in the southeast US and its April when I am writing this review. With that said; its been in the 70s/80s regularly so feel like I can comment fairly on its performance in warmer temps; though I'll have to try and remember to comeback and update it for the summer season when AC is on full blast and humidity is higher (and the inevitable "swamp butt" sets in).
In the first week I've found the chair to be comfortable to sit in. It is wide enough and the arm rests to the side enough that I can sort of lean left and right without much trouble. I like this wider seat and armrests compared to chairs with more narrow span as I tend to shift and move a lot while sitting.
The PU leather material feels nice against my clothes and does "grip" the clothing which helps with my tendency to want to slide forward when sitting. At first I thought the "grip" would be annoying but I sort of like it after a week of using the chair.
The cushion material is comfortable for sitting and rebounds well after I've been sitting in it for a long period of time. The armrests feature the same sort of cushion and PU leather material which makes them comfortable to sit back and lean on.
For the price I think this is a solid office chair and a comfortable option. Longevity will only be tested over time so I'll see if I can remember to update this review over time. I am happy with the chair in my office.
A few downsides about the chair that I have:
- The wheels are very stiff and don't roll as smoothly as I'd like. They stay put when I am sitting at the desk but when I need to push away they take a bit of force.
- The arm rests can't be adjusted for height. They are one height and can only be moved out of the way completely. For someone like who likes to lean or shift sides this is less than ideal as I'd normally make adjustments to the armrests. With that said it is nice to be able to move them out of the way completely when I don't need them.
- Lumbar support could be better. I will be using additional support in the chair for my back.
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Reviewed in the United States on April 13, 2026
★★★★★ 5
Good for sitting on it daily. Not that soft as expected
Color: Dark, Color: Dark
I’ve been using this chair for a few days now and overall it’s pretty solid for a home office setup, especially for the price range. Assembly took me about 30–40 minutes alone and all the parts were labeled clearly. Nothing arrived damaged and the instructions were easy to follow, though attaching the backrest was a little awkward with one person.
Once assembled, the chair looks clean and professional. The PU leather feels smooth and the cushioning is definitely thicker than cheaper office chairs I’ve tried. It’s comfortable for working several hours at a time, although I wouldn’t describe it as super plush or “sink-in” soft. The seat has a firmer feel, which may actually be better for posture during long work sessions.
The ergonomic support is decent. The backrest follows the shape of your back fairly well and gives noticeable lumbar support compared to flat office chairs. I spend a lot of time at my desk and this chair helped reduce some lower back discomfort. The reclining and rocking feature is also nice for quick breaks between meetings or while watching videos. It doesn’t recline extremely far, but enough to relax a bit without feeling unstable.
One feature I ended up liking more than expected was the flip-up armrests. They make it easy to slide the chair completely under the desk and also give more room if you like sitting cross-legged occasionally. The chair rolls smoothly and feels stable even when leaning back.
A couple downsides though. The faux leather can get warm after extended sitting, especially in a warmer room. The mesh section helps airflow a little, but not enough to fully prevent heat buildup. Also, while the chair is comfortable, it doesn’t quite feel “premium executive” level in terms of materials. Some of the plastic components and armrests feel more mid-range than luxury.
Overall, I’d say it’s a good value office chair with solid comfort, decent back support, and practical features. Great for everyday work or study use, just keep expectations realistic if you’re looking for a super high-end executive chair feel.
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Reviewed in the United States on May 6, 2026
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