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Description
Human PARK2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Parkinson's Disease Protein 2 (PARK2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Parkinson's Disease Protein 2 (PARK2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Parkinson Disease Protein 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Parkinson's disease protein 2, also known as Parkin, is a 465-amino acid E3 ubiquitin ligase encoded by the PARK2 gene. Parkin plays a key role in ubiquitination—the process by which molecules are covalently labeled with ubiquitin (Ub) and targeted for degradation by proteasomes or lysosomes. Upon cell injury, Parkin recognizes proteins on the mitochondrial outer membrane and mediates the clearance of damaged mitochondria through autophagy and the proteasome. Parkin also enhances cell survival by inhibiting both mitochondria-dependent and -independent apoptosis. Mutations are associated with mitochondrial dysfunction, leading to the abnormal metabolism that contributes to neuronal death and tumorigenesis in Parkinson's disease. This protein is a component of the multiprotein E3 ubiquitin ligase complex, which in turn is part of the ubiquitin-proteasome system, which mediates targeted protein degradation. Mutations in this gene are known to cause a familial form of Parkinson's disease, autosomal recessive juvenile Parkinson's disease (AR-JP). Furthermore, Parkinson's disease has been described as requiring mitophagy (autophagy of mitochondria). | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.8 ★★★★★
Based on 13 reviews
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Product Reviews
★★★★★ 5
2 prong to 3 prong power strip. Devices share common ground bus. Green light on switch. Compact plug
Size: 5FT, Color: White 2prong, Size: 5FT, Color: White 2prong
This power strip has no ground. It allows you to plug a 3 prong device into the strip which has a 2-prong plug going into your outlet. You'll want to make sure you understand the implications of that if you use this. For my use I'm using the power strip to have the convenience of using my 3 prong extension cords for ungrounded devices.
The power strip is composed of the main body with 11 outlets plus 3 USB and 3 USB-C ports. There's a switch to turn on or off the power switch and the switch has a green light when turned on. On the back side are holes for optional surface mounting as well as the power ratings. The USB ports are all standard 5V ports. While the ground plug on the 3-prong outlets is not connected through the power cord the outlets on each side share a common bus on the power strip itself. Thus if any device plugged in on that side has power going into the ground prong it will energize the ground on all devices connected with a ground prong on that side. The body measures approximately 8.125" by 4.125" by 1.25" thick. I weighed the power strip with cord at 15.38 ounces.
The power cord is approximately 4.9' long with a compact right angle plug. The plug is polarized meaning you cannot plug it in inside down. Looking at a 3-prong plug if the ground prong is at the bottom the cord goes down to the right at about a 45 degree angle. The plug is compact and you can plug 2 of these in on the same 2 outlet wall outlet. The cord is printed with the wire information and contains 2 16 AWG conductors. The rated power seems appropriate for the size of the power cord.
The outlet looks and feels nice and handles a lot of devices.
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Reviewed in the United States on May 20, 2026
★★★★★ 5
Good value for a high quality product with some possible safety compromises
Size: 5FT, Color: White 2prong, Size: 5FT, Color: White 2prong
This power strip is a practical solution for anyone living in an older home that still has two‑prong outlets. Many older houses were built long before grounded wiring became standard, so finding a way to expand your available outlets without rewiring the entire room can be a challenge. This adapter is designed specifically for those situations, and it provides a surprising amount of capability in a compact unit.
Before using it, I wanted to be sure it was safe to plug a three‑prong device into a two‑prong outlet. It is legal to use an adapter like this in the United States, but only when you understand its limitations. A two‑to‑three‑prong adapter does not create a true ground. The product page notes that this model is built to UL 1363 standards and includes surge and overload protection, which helps add a layer of safety for older outlets. Still, it is not a substitute for proper grounding, and anyone who wants full protection should eventually have an electrician install grounded outlets.
As for the device itself, it is well designed and easy to use. The main body includes 11 outlets, along with 3 USB ports and 3 USB‑C ports for charging phones, tablets, and other small electronics. All USB ports are standard 5V. There is a power switch with a green indicator light, and the back of the unit includes mounting holes if you want to secure it to a wall or desk. The size is compact—about 8 inches long, 4 inches wide, and just over an inch thick—so it fits neatly in tight spaces.
Overall, this is a good value for anyone who needs more outlets and charging options in a home with older two‑prong receptacles. It offers convenience, versatility, and thoughtful safety features in one organized unit.
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Reviewed in the United States on May 24, 2026
★★★★★ 4
Great many outlets but insufficient usb power for most needs
Size: 5FT, Color: White 2prong, Size: 5FT, Color: White 2prong
I absolutely LOVE how many ports/outlets this extension unit has. It has usb-As, usb-Cs, AC outlets, on/off switch... In that regard, it's perfect.
But. If you're looking to charge your phone or laptop, for example, by plugging your charging cord without an adapter, directly into a usb-c or usb-a port, you'll be completely out of luck. When I first got this I plugged in my laptop cord directly into one the usb-c ports while using it. I eventually, fairly quickly I must say, realized that it was as though the laptop wasn't plugged in at all. It even seemed to lose power faster than if it weren't plugged in! And, it was the only thing plugged in at the time. So it might just be me expecting a charge instead of the opposite, but the issue stands. I had to go and find my A/C adapter and charge my laptop the same way I would without this unit, as if I was plugged into the wall.
Now I'm sure there are people out here surprised that anyone would be confused by having to use an adapter to charge a phone or laptop while using this unit. My confusion comes from the unit having as many usb-c and usb-a ports as it does. I don't think it's a stretch to assume, as I did, that the point of having so many ports is to actually use them. One reason I haven't rated this lower is that I knew of the ports low voltage and wattage, so I wasn't completely surprised by having to use an adapter, though I WAS surprised by how completely useless the usb ports seem to be. Also, one description picture shows a phone being charged WITHOUT an adapter. And this does charge my phone but only if I'm not using it. It stays at pretty much the same battery level if I'm using it while charging it. So I don't think I'm 100% at fault for being surprised
If you will be using the usb ports to charge a small appliance, a groomer, a shaver, a toothbrush? I'm sure this will do well. I don't have any of those so I guess I won't be using the usb ports at all or will use them only if I'm not in a rush to get a fast charge.
Once the initial surprise subsided, I'm still glad I got this. It has ELEVEN A/C outlets, a flat plug that can fit just about anywhere and behind any piece of furniture, and I could still charge my phone (thought not my laptop) in a pinch. I think the price at the time of this review is fine for those features. 3.5 stars.
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Reviewed in the United States on May 21, 2026
★★★★★ 5
Many Outlets in One Compact Device!
Size: 5FT, Color: White 2prong
The number of outlets is impressive for its size, and the USB ports are super convenient for phones, tablets, and other small devices. Everything charges steadily without overheating, and the 5 ft cord gives enough flexibility to place it where I need it. I also really appreciate the compact design. Even with so many plugs available, it doesn’t feel bulky or cluttered. It fits nicely behind furniture and keeps everything organized instead of having a mess of adapters everywhere. It’s especially perfect for older homes or apartments with only a couple of outlets per room. While it’s not a substitute for true grounded outlets for heavy appliances, it works perfectly for everyday electronics.
These items are typically fine:
Phone chargers
Laptops
LED lamps
Routers / modems
TVs (modern, low draw)
These devices are double-insulated or low risk, so they don’t rely heavily on grounding.
Avoid using this for:
Space heaters
Air conditioners
Microwaves
Refrigerators
Power tools
Anything metal-bodied or high-power
Without a real ground, a fault could energize the casing and increase shock risk.
Overall, this is a solid, reliable, and incredibly convenient power strip. I’d definitely recommend it to anyone needing more outlets and USB charging in one simple solution for appliances motioned above that are safer to use with relatively better price.
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Reviewed in the United States on June 1, 2026
★★★★★ 5
Perfect Sofa-Side Charging Hub
Size: 5FT, Color: White 2prong, Size: 5FT, Color: White 2prong
I picked this power strip mainly to use next to my sofa, where I usually charge my tablet, phone, and laptop while relaxing in the evening. Having 11 AC outlets and multiple USB ports in one place has made that corner much more organized, and I no longer have chargers scattered everywhere.
The layout is well thought out. Larger plugs don't block neighboring outlets, and the flat angled plug fits nicely behind furniture. The 5-foot cord gives enough flexibility to position the strip where it's most convenient. I also like having both USB-A and USB-C ports available for smaller devices. For phones, tablets, earbuds, and similar electronics, the built-in USB ports have been very useful.
For my everyday use, charging personal devices and powering small electronics, the unit has performed as expected. The overload protection switch and built-in safety features provide additional peace of mind.
Overall, this has been a practical solution for adding more outlets to an older two-prong receptacle while keeping my charging area tidy and easy to access.
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Reviewed in the United States on June 2, 2026