Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 30 - Sep 4
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human PIIICP ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against procollagen III C-terminal propeptide (PIIICP). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of procollagen III C-terminal propeptide (PIIICP) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Procollagen III C-Terminal Propeptide ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Type III procollagen carboxyl-terminal propeptide (PⅢCP) is a homotrimer, or a protein composed of three identical peptide chains (monomers), each of which is called the α1 chain of type III collagen. Formally, these monomers are referred to as type III collagen, α-1 chain, and are encoded by the COL3A1 gene. It is one of the fibrillar collagens, with a long, inflexible, triple-helical domain. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.9 ★★★★★
Based on 20 reviews
Sort
Product Reviews
★★★★★ 3
Not interested
Size: Large (Pack of 1)
My dog is not very interested in it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 13, 2026
★★★★★ 5
Best Dog Bone
Flavor Name: Beef, Size: Large
This is one of the best bones I've seen and I have always lived with dogs. The shape makes it so ergonomic for the dog, both of mine hold the bone down with both paws then chew the part standing up. One of my dogs was able to enjoy this while wearing a cone after surgery, supporting her natural behavior and keeping her busy. These are great for our 11 month old puppy because he was struggling to figure out how to brace the bone without it rubbing his paws, so was bracing on furniture and getting in trouble. Now he can hold the T part easily and doesn't need to use other objects he shouldn't chew by accident. They are durable, too. I got these about 2 months ago and they are barely worn with a rotty/pit/Shepard mix male puppy. If you know dogs, you know that means this is a solid durable bone! I will for sure keep these on hand for years as they are a preferred chew in my house!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 1, 2026
★★★★★ 5
Doggy Delight
Flavor Name: Beef, Size: Medium
Ever felt like your dog’s chew toys are on the fritz, ending up shredded like yesterday’s sandwich? The SPOT Bam-bones PLUS T Bone is here to save the day, offering a durable and tasty solution for aggressive chewers and teething puppies alike. These bamboo fiber and nylon chews are like the super-tough treats your dog dreams of, providing hours of gnawing joy without the splintering mess.
My bulldog, who treats his chew toys like they’re part of a survival game, has found his perfect match in these T Bones. The beef flavor is irresistible, making them a favorite among picky pups and ensuring they stay engaged without turning into edible confetti. The 6-inch size is perfect for keeping his jaws busy without overwhelming his mouth – a win-win for both of us.
The non-splintering design is a blessing, preventing any painful encounters with broken chews and keeping our home safe from unexpected trips to the vet. Plus, the durable construction means these chews last longer than my patience during a doggy tantrum – and that’s saying something!
However, the T Bones can sometimes feel like they’re too tough, requiring a bit of extra chewing to fully enjoy – turning every chew session into a mini workout for your dog’s jaws. Additionally, the price point is slightly higher than standard chews, but the durability and quality make it worth every penny.
Overall, the SPOT Bam-bones PLUS T Bone is a fantastic choice for dog owners looking to provide their pets with a long-lasting and enjoyable chew experience. It’s tough, tasty, and built to withstand even the most enthusiastic chewers. A solid 5-star rating for its durability and flavor, with a tiny star lost to its occasional toughness challenges.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 23, 2024
★★★★★ 5
The Ultimate Solution for Power Chewers!
Flavor Name: Beef, Size: Medium
I have a Pitbull mix who can usually destroy "tough" toys in minutes, but the Bam-Bones Plus is a total game-changer. The bamboo and nylon blend is incredibly dense; it satisfies that natural urge to chew without splintering like real wood or falling apart like rubber. It wears down slowly and safely, giving my dog weeks of use rather than minutes.
Smart, Functional Design
The T-bone shape is brilliant because of the "paw-grip" base—it makes it so easy for my dog to hold it steady while she works on it. I’ve also noticed her teeth looking much cleaner since we started using this, as the raised textures act like a natural toothbrush. Plus, the infused beef scent keeps her interested much longer than a plain nylon bone ever could.
Final Verdict
This is a must-have for any owner of a large or aggressive chewer. It’s safe, long-lasting, and provides excellent mental stimulation.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 16, 2026
★★★★★ 4
Fantastic Durable Chew Toy That My Dog Loves!
Flavor Name: Beef, Size: Medium
Absolutely thrilled with this dog chew toy! It’s extremely tough and durable — perfect for aggressive chewers and keeps my pup entertained for a long time. The bamboo fiber and nylon design feels sturdy yet gentle on the teeth, and it even helps with dental hygiene by massaging gums while he chews. My dog goes crazy for this bone and it lasts way longer than other chew toys we’ve tried. Great quality and great value — highly recommend for any dog
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 17, 2026