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Description
Mouse IL-2 ELISA KitProduct Specification Theory Double antibody sandwich enzyme linked immunosorbent assay was used in this kit. Specific anti mouse IL 2 antibody was precoated on a high affinity microplate. The standard substance, test sample and biotinylated detection antibody were added into the microplate Wells. After incubation, the IL 2 present in the sample combined with the solid phase antibody and detection antibody to form immune complexes. After washing to
Product Specification
| Theory | Double antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse IL-2 antibody was precoated on a high-affinity microplate. The standard substance, test sample and biotinylated detection antibody were added into the microplate Wells. After incubation, the IL-2 present in the sample combined with the solid-phase antibody and detection antibody to form immune complexes. After washing to remove the unbound material, the horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, the chromogenic substrate was added and the color was developed in the dark. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm). | |||||||||||||||||||||||||||
| Composition |
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| Background | Interleukin-2 (IL-2, also known as TCGF) is a 15-16 kda monomeric α-helical glycoprotein belonging to the type I cytokine family. Mouse IL-2 precursor is a 169-amino acid (AA) peptide containing a 20-amino acid signal sequence and a 149-amino acid mature chain. There is an additional potential site for O-linked glycosylation, as well as a polyglutamine region at residues 35 to 46. It is worth noting that mouse IL-2 has multiple alleles, resulting in differences in the number of glutamine at amino acids 21-46 and the number of TSSS repeats in the mouse IL-2 sequence (SwissProt#:P04351). The amino acid sequence of mature mouse IL-2 shared 56% and 73% identity with human and rat IL-2, respectively, with the major difference located in the polyglutamine region. There is some cross-reactivity between mouse and human IL-2. Functionally, IL-2 was originally recognized as a T-cell growth factor and, therefore, was used in the treatment of cancer and AIDS patients, as well as to enhance the immune response. However, these efforts have had limited effect. "Experimental studies have shown that mice lacking IL-2 or its receptor also develop lymphoproliferation and autoimmune diseases, suggesting that IL-2 is a growth-limiting rather than growth-inducing factor." Recent studies have shown that the real cause of lymphocyte proliferation is the inability to produce CD4+FoxP3+CD25/IL-2Rα+Treg cells. It is important to note that now IL - 2 is considered successful induction of CD8 + memory cells necessary for memory response. It is well known that mammalian cells expressing IL-2 include CD4+ and CD8+T cells, NK cells, NKT cells, and dendritic cells. The IL-2 receptor is complex and consists of three different subunits. The two subunits can be combined with ligands, called IL - 2 r alpha and beta IL - 2 r. IL-2Rα is 55kDa and binds IL-2 with low affinity. IL - 2 beta for 75 kda, is part of the IL - 15 receptors, moderate affinity with IL - 2. Signal transduction is mediated by IL-2Rβ and a 64kDa common γ chain (γc). Gamma c and IL - 4, 7, 9, 15-21 and Shared receptors. Signal transducerγc does not bind to IL-2 but heterodimerizes with IL-2Rβ to form a functional IL-2 receptor. Heterologous trimer alpha beta gamma receptor may be combined with IL - 2 c, forming R alpha beta complex R. | |||||||||||||||||||||||||||
| General Notes | 1. Please use the kit within the validity period. 2. Components of different kits and different batch kits should not be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. If the cell culture supernatant sample needs to be diluted, except for the last step, diluent (1×) is used. "In addition to dilution, cell culture medium may be used for other intermediate dilutions." 4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
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| Storage Temp. | Unopened kit, 2-8° C Storage | |||||||||||||||||||||||||||
| Test Range | 15.6pg/mL-1000pg/mL |
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4.2 ★★★★★
Based on 22 reviews
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Product Reviews
★★★★★ 5
Step In Look Good
Size: 11.5, Color: Cognac Napa Leather
I am wearing these shoes as I write this review, and they look and feel great. I have a bit of a disability with drop foot on the right leg and bilateral neuropathy and have been wearing step in shoes for about 10 years. But the first company that introduced leather dress and casual shoes stopped making them and now only makes sport and casual shoes. So I have been searching for some that meet my need for shoes like this and this company has given me three pairs, so far. Easy to get into, comfortable to wear, and good looking. And they take polish very well, too. (Remember how to do that?). We may buy another pair or two in different styles as well.
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Reviewed in the United States on April 26, 2026
★★★★★ 4
Runs tight. Skinny and smaller than other brands.
Size: 8.5, Color: Brown
Comfy and lots of support. Cushiony. Beware: the brown I ordered runs small. Tighter than other brands.
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Reviewed in the United States on April 23, 2026
★★★★★ 5
Right Decision
Size: 12 Wide, Color: Cognac Napa Leather
Very comfortable. Classic style.
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Reviewed in the United States on June 11, 2026
★★★★★ 1
Leather peels right off
Size: 8, Color: Black
Very poor quality - shoes are ruined after a couple months
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Reviewed in the United States on May 28, 2026
★★★★★ 5
Good purchase
Size: 9.5, Color: Black
Value and quality was good
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Reviewed in the United States on May 16, 2026