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Description
SRP54 Protein, HumanProduct Specification Synonyms Signal Recognition Particle Subunit SRP54 Amino Acid Sequence Met1 Met504 with a C terminal flag tag Expression System E. coli Molecular Weight Approximately 56. 84 kDa, a single non glycosylated polypeptide chain containing 515 amino acids. Purity 96% by SDS PAGE or HPLC. Endotoxin <0. 01EU g Conjugation Unconjugated Physical Appearance Liquid Storage Buffer 20 mM PB, with 50 mM NaCl, 150 mM Imidazole, 50% (v v)
Product Specification
| Synonyms | Signal Recognition Particle Subunit SRP54 |
| Amino Acid Sequence | Met1-Met504 with a C-terminal flag tag |
| Expression System | E.coli |
| Molecular Weight | Approximately 56.84 kDa, a single non-glycosylated polypeptide chain containing 515 amino acids. |
| Purity | ＞96% by SDS-PAGE or HPLC. |
| Endotoxin | <0.01EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM PB, with 50 mM NaCl, 150 mM Imidazole, 50% (v/v) Glycerol, pH 7.4. |
| Reconstitution | Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage | For long term storage, the product should be stored ≤ -20℃. |
Background
SRP54, as part of the SRP complex, associates with the SRP receptor (SR) component SRPRA to target secretory proteins to the endoplasmic reticulum membrane. SRP54 is component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the co-translational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER) , It Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein) . Recombinant Human SRP54 is a 56.84kDa protein globular protein containing 515 amino acid.
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