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Description
Human TNF-α ELISA KitProduct Specification Species Reactivity Human Theory This kit adopts double antibody sandwich enzyme linked immunosorbent detection technology. Specific anti human TNF antibodies were pre coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, TNF present in the sample binds to the solid phase antibody and the
Product Specification
| Species Reactivity | Human | |||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human TNF-α antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, TNF-α present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Synonym | APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, tnfa, tnf-a, TNFalpha, TNF-alpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor, tumor necrosis factor-alpha | |||||||||||||||||||||||||||
| Composition |
Please use it within the validity period of the kit (new and old products are shipped randomly)
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| Background | Human TNF-α is a 26kDa type II transmembrane protein consisting of a 35 amino acid (aa) intracellular domain, a 21aa transmembrane segment, and a 177aa extracellular domain (ECD). In the ECD region, human TNF-α has 97% amino acid sequence homology with macaque monkey, and 71-92% amino acid sequence homology with cattle, dog, cotton rat, horse, cat, mouse, pig and rat. It can be expressed by a variety of different cells such as immune cells, epithelial cells, endothelial cells, tumor cells. TNF-α can induce lysis of tumor cells and virus-infected cells, and binds to soluble TNFRI to generate transmembrane transfer signals of downstream cells. TACE/ADAM17 can cause the shedding of TNF-α-containing cell membrane to release active TNF-α cytokine. It is a trimer composed of TNF-α extracellular soluble structure with a molecular weight of 55 kDa. TNF-α has two receptors: TNFRI and TNFRII. TNFRI has a molecular weight of 55-60kDa and is widely expressed; TNFRII has a molecular weight of 78-80 kDa and is limited to hematopoietic cell expression. Both are expressed as homotrimers; TNF-α and TNFRI and TNFRII bind with similar affinities and can promote the activation of NFκB. However, only TNFRI has a cell death domain, which can trigger apoptosis. Both soluble receptors are released into human serum and urine and can neutralize the activity of TNF-α. |
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| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. |
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| Storage Temp. | Kit unopened, stored at 2-8 °C. | |||||||||||||||||||||||||||
| Test Range | 15.6pg/mL-1000pg/mL |
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4.6 ★★★★★
Based on 29 reviews
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Product Reviews
★★★★★ 4
Purse for wedding
Color: Blush
Very inexpensive and pretty. Quality is ok but a one time to a wedding. I hooked a shoulder fancy strap on it as I need 2 hands for my walker. Size perfect. Great value for the money
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 1, 2026
★★★★★ 5
Pink beautiful purse
Color: Hot Pink
Beautiful deep pink purse for evening attire for young girl or asult
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 22, 2026
★★★★★ 5
Is this the one for you? It was for me!
Color: Black, Color: Black
Okay so if you are like me and shop last minute before a major event then this is perfect. I went through pages and pages on here to find a bag that let's be honest here will only be used once and put away. So I decided on this one because of the style and color as it will go perfectly with my emerald green dress that I am wearing to my bestie's son's wedding literally next month. The size is perfect and the quality is on point. Yes the bag is a little stiff when opening for the first time but if you plan to use this constantly then it should be more pliable over time. This will hold what I intend to take with me which is just the basics so the functionality will be great and the fact that it travels well is another point for this evening bag. Big value for the money and easy to use. If you like it buy it, if not find one that suits your needs and style and budget. This is a keeper.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 3, 2025
★★★★★ 3
Beware of the smell
Color: Black
Starting with the likes about this product:
1. Beautiful design
2. Large enough for my cell phone
3. Most important it matched my HEEZ Women's Ballet Flats Shoes
Now for the dislikes:
1. The chemical smell coming from the bag was soooo strong my eyes watered and my nose burned just a little. There is no way I could carry this purse with out that smell wafting through the air. IT WAS THAT STRONG.
2. The flap was a little stiff to bend, so closing was a little hard to get it in position. I'm sure if you opened and closed it a few time it would loosen up.
Bottom line I only gave it a 3 star because of the smell and I am retuning it. Not sure if you air out the bag for a few days if the smell would go away but I didn't want to risk it.
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Reviewed in the United States on October 30, 2024
★★★★★ 5
Beautiful
Color: Black
Absolutely beautiful. Matches my gown perfectly
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Reviewed in the United States on May 18, 2026
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