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Description
Ki67 Recombinant Mouse mAb (SDT-606-7)Product Specification Host Mouse Antigen Ki67 Synonyms Proliferation marker protein Ki 67, Antigen identified by monoclonal antibody Ki 67, Antigen KI 67, MKI67 Immunogen Synthetic Peptide Location Nucleus Accession P46013 Clone Number SDT 606 7 Antibody Type Recombinant mAb Isotype IgG1 Application IHC P, ICC, IF Reactivity Hu Purification Protein G Concentration 2 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40%
Product Specification
| Host | Mouse |
| Antigen | Ki67 |
| Synonyms | Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, MKI67 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P46013 |
| Clone Number | SDT-606-7 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG1 |
| Application | IHC-P, ICC, IF |
| Reactivity | Hu |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20°C as supplied |
Dilution
| application | dilution | species |
| IHC | 1:250 | |
| ICC | 1:500 | |
| IF | 1:200 |
Background
The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation, and can be used in immunohistochemistry. It is strictly associated with cell proliferation. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent in resting (quiescent) cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. In breast cancer Ki67 identifies a high proliferative subset of patients with ER-positive breast cancer who derive greater benefit from adjuvant chemotherapy.
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