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Description
Human PML ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a promyelocytic leukemia protein (PML) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of promyelocytic leukemia protein (PML) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Promyelocytic Leukemia Protein ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Promyelocytic leukemia protein (PML), also known as MYL, RNF71, PP8675, or TRIM19, is the protein product of the PML gene. This protein is a tumor suppressor required for the assembly of numerous nuclear structures, called PML nuclear bodies, which form within the chromatin of the cell nucleus. These nuclear bodies are present in mammalian cell nuclei, with approximately 1 to 30 per nucleus. The protein encoded by this gene is a member of the tripartite motif (TRIM) family. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.5 ★★★★★
Based on 25 reviews
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Product Reviews
★★★★★ 5
Good Basic Athletic Shirt
Size: Small, Color: Gray (002)/Black
These shirts (I have six) are all they should be, and, for the price, an excellent choice for a regular workout or running shirt. Nothing too flashy. Washes well. Doesn't shrink or wrinkle. May run a tad small.
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Reviewed in the United States on June 7, 2026
★★★★★ 5
Fits great
Size: 3X-Large, Color: Black, Size: 3X-Large, Color: Black
Ah, let me spin you a yarn about the Under Armour Men's Tech 2.0 Short-sleeve T-shirt – the sultan of comfort, the maestro of moisture-wicking, and the unsung hero of casual wear that will make you question why you ever settled for less in the realm of T-shirts.
First and foremost, let's talk about the fabric – it's like wrapping yourself in a cloud woven from the threads of a thousand cotton candy dreams. The Tech 2.0 T-shirt caresses your skin with the tenderness of a gentle breeze, making you wonder if you accidentally stumbled upon a secret society of clothing wizards who specialize in the art of softness.
The fit is like a custom-tailored suit for your torso. It hugs you in all the right places without making you feel like a sausage casing or a misplaced balloon animal. I've worn it for everything from impromptu dance-offs to intense staring contests with the refrigerator, and it never once betrayed my aesthetic sensibilities. It's the kind of fit that says, "Yes, I work out, and yes, I also enjoy pizza. Balance, my friends, balance."
Now, let's delve into the realm of moisture-wicking technology – a.k.a. the superhero cape of T-shirt features. I've worn this shirt through sweaty gym sessions, summer heatwaves, and surprise encounters with old acquaintances that induce nervous perspiration. And yet, it remains drier than a stand-up comedy show in a desert. It's practically a magical force field against sweat – the kind of sorcery that leaves you feeling fresher than a daisy in a rain shower.
But the real showstopper is the Anti-Odor technology. It's like the T-shirt gods sprinkled a bit of enchantment into the fabric, ensuring that no matter how much you exert yourself, you'll smell as fresh as a mountain breeze. I've worn it for consecutive days – don't judge; laundry is a social construct – and emerged smelling like a field of wildflowers.
And let's not forget the humor in this T-shirt masterpiece. The Under Armour logo is like a subtle flex, a tiny symbol that says, "I take my comfort seriously, but I'm also low-key athletic." It's the T-shirt equivalent of a wink and a nod, a fashion statement that knows how to have a good time.
In conclusion, the Under Armour Men's Tech 2.0 Short-sleeve T-shirt isn't just a T-shirt; it's a lifestyle upgrade. With a comfort level that defies earthly standards, moisture-wicking prowess that mocks the very concept of sweat, and a dash of humor in its branding, it's the shirt you never knew you needed until you experienced the cottony embrace of greatness. So, fellow T-shirt connoisseurs, let the Tech 2.0 redefine your torso's expectations – where every wear is a journey into the luxurious realms of fabric excellence!
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Reviewed in the United States on November 9, 2023
★★★★★ 5
Superb running shirt
Size: Medium, Color: Steel Light Heather (036)/Black
I really love this shirt, so much I ordered a second one. I'm between a medium and large and found the medium was the best fit; not too tight. The large was a bit on the big side for me, but I kept it anyway, it's not so big it swallows you up.
Even when sweaty, it doesn't cling to you like some of the running shirts I've tried.
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Reviewed in the United States on May 28, 2026
★★★★★ 3
Great fit and comfort, tho large variation in fabric features concerns me UPDATE 2Y LATER
Size: X-Large, Color: Academy Blue (408)/Graphite
Have bought a dozen of these, and for the most part aren't disappointed: exactly what I want in a moisture-wicking workout shirt: sturdy enough for weekly machine washing (front-loader, no agitator btw) but light and enough airflow to cool when hot, but still comfy down to high 60F w/o an add'l layer.
Am a massage therapist so constantly work in conditions of strenuous physical activity for some parts of my work, in an office w/ A/C temps set to 68 -- cotton is pitifully bad at meeting comfort requirements for my use case; only wicking synthetic wear makes any sense.
However... one thing I do notice about this shirt, which retails for $25 -- not cheap -- is the fabric seems outsourced to many suppliers, who all have their own idea of how to manufacture the base fabric. Some are more translucent than others, some far more stretchy and Lycra-like, and a variety of what 'navy blue' is.
Largely this doesn't matter for me, as the dark colour reveals much less than lighter ones or white... but for anyone trying to conceal scars, tattoos, body hair or other physical traits you want concealed under clothing... the random chance you may receive a shirt with so much see-thru, one can easily make out physical details of the body underneath (especially in bright light as outdoors)... this may turn you off this UA line -- one star taken for QC issues.
Four stars ⭐️⭐️⭐️⭐️ _
UPDATE 26 MAY 2026:
Have been wearing this set of shirts for 2y now, so can confidently claim pros and cons about the product.
Pros:
- generally consistent after weekly washes over 2y (front-loading machine-washed warm, and machine-dried moderately warm w/ dryer sheets)
- colour has stayed very colourfast
- surprisingly resilient to both constant on-off during the day (switching shirts between clients when heavy client load to help prevent perceptible body odour) and hanging on hooks to dry what perspiration does absorb into them
- very resistant to pilling -- which is not something I can claim with a lot of other wicking activewear
- logos even in second-to-hottest dryer setting have not melted or worn at all -- whatever formulation UA uses for its silkscreened logos, needs to be an industry standard
- does not in any way retain odours thru a wash (again, unlike many other brands of activewear I used to wear regularly)
Cons:
- the variation in type of fabric used as the base for this shirt, is the biggest criticism I have -- some wick moisture so well (probably UA's original spec) that it performs perfectly as claimed, with no apologetic action from me to compensate for... but others actually insulate -- causing a pool of perspiration to form between fabric and skin, negating its advantage over cotton blends. Inconsistency means it's a lottery on which of these shirt types you receive... which at $25 a pop is money I don't have, to spend for a company's lack of proper QA with overseas suppliers
- Related: some shrink, most don't. But again, the one that does shrink (esp if it comes already small as quality control drift within UA's already proven to me), is a $25 penalty per against this shirt, perhaps shopping elsewhere would solve. Can't face clients with the bottom of my belly sticking out, when it fit perfectly first bought
So more more pros than cons, but the cons are both showstoppers. Hope UA takes this criticism and actually improves the product as global nonsense jacks prices up everywhere, as over 2y they haven't changed much.
Taking a star for two showstoppers unaddressed 2y, so lenient demotion to 3 stars, since their pros don't require me throwing away *all* their shirts for showstoppers above, just $100 - $125 worth ⭐️⭐️⭐️ _ _
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Reviewed in the United States on January 7, 2025
★★★★★ 5
Comfort Color is a trusted brand for comfort and durability. These are great!
Size: X-Large, Color: Blue Spruce, Number of Items: 2
Great price for two of these high quality Comfort Colors tees. I really love the color (a blue/green blend). So pretty. I find their sizing to be true to size and the quality to be great. Washes well. Dries well. Is comfortable and soft without being flimsy and thin. While these are called "heavyweight", it just means a nice, solid tee but isn't overly hot to wear. Very happy with them and I buy these for printing because they do well with decals and washing. These do not get wrinkly in the dryer either.
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Reviewed in the United States on June 5, 2026