Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 30 - Sep 4
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human ANGPTL8 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with angiopoietin-like protein 8 (ANGPTL8) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of angiopoietin-like protein 8 (ANGPTL8) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Angiopoietin Like Protein 8 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | ANGPTL8 (also known as lipoprotein 8) is a protein encoded by the C19orf80 gene. The encoded 22 kDa protein contains an N-terminal secretion signal and two coiled-coil domains and is a member of the angiopoietin-like (ANGPTL) family of proteins. However, unlike other ANGPTL proteins, ANGPTL8 lacks a C-terminal fibrinogen-like domain, making it an atypical member of the ANGPTL family. Like ANGPTL4 and ANGPTL8, it has the ability to inhibit lipoprotein lipase (LPL). | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.0 ★★★★★
Based on 29 reviews
Sort
Product Reviews
★★★★★ 5
Totally worth it!!
So I have combination skin with a tendency toward closed comedones and I decided to try this product because I heard that azelaic acid helps with closed comedones. Honestly, I didn’t have very high expectations because my closed comedones have been very stubborn. I was already using the COSRX BHA before introducing this product, but it worked very slowly, so I wasn’t really getting the results I wanted from that product alone. Let me tell you that once I introduced this product, I finally started seeing the results I was looking for. I have noticed that my closed comedones have been resolving way faster than when I was only using the BHA and my skin also feels softer and overall looks healthier. I also want to mention that even though I don’t get inflamed breakouts very often, when I do get one, I feel like this product helps it calm down and go away much faster. I did start incorporating it slowly, and I highly recommend that anyone who wants to start using this product do the same and not rush into using it every day right away. Despite that, I personally haven’t had any issues with irritation at all and I actually find it very soothing, but you always need to be careful when adding new actives to your routine. I am currently using this product 4-5 days a week, once a day, and on the days I do not use it, I use my BHA instead. I also find that this product lasts a long time, since you only need like five drops for the entire face and the consistency is very watery. I’ve been using it for around two months, and there is still plenty left. Overall, I really recommend this product because it has helped me a lot, and while I am not sure if this alone would be enough to treat closed comedones, I would definitely say it is a great complement to other treatments.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 27, 2026
★★★★★ 5
Changed my skin in 5 days
This stuff transformed my skin in 5 days! I’ve had issues with random breakouts and redness ever since I turned 40 and have had difficulty finding a product that actually helps those issues. I incorporated this product into my daily routine for the last 5 days and my skin is clear, moisturized, super soft and redness/breakout free. I use this after washing my face and applying toner; I use just enough to cover my face as a little goes a long way. It has a very loose, almost oily consistency but spreads easily and doesn’t make your skin feel greasy at all. Once it’s absorbed into my skin, I apply my moisturizer. I would definitely recommend this to anyone with skin redness and dryness, it’s a game changer!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 15, 2026
★★★★★ 5
I think this will help my rosacea redness!
Update: after using this 2 months, I will be reordering. The rosacea redness to my cheeks and nose have drastically reduced. I use it every other day. I tried a routine of on alternating mornings using vitamin c one day and this Anua azelaic acid the next. Cleanse, tone, azelaic acid, and then whatever I'm doing next (including SPF if I'm going outside). I don't use it at night, preferring a different routine with actives at night.
I don't use the hypochlorous acid on my face very often at all now so the Anua is doing the work of hydrating and helping calm my skin.
Note that I have aging skin that likely could be prone to dryness but I am meticulous about my skincare so my skin isn't dry - so though they advertise it's for blemish-prone skin, I use it for rosacea redness, and alternating plus hydrating before and after azelaic acid is what works for my skin.
------
The first time I tried this over my entire face, it made my face immediately red so I considered returning it. However, I had used other serums and thought maybe it was just too much at once for my rosacea-prone skin, and that I used too much as well. (I will add that I did a small patch test along my jawbone and let that absorb for hours - always do a patch test of an unfamiliar product!)
After a couple of days, I tried it again, and I am impressed! This time, I hadn't used my tretinoin the night before - since I just went up in strength, my skin has been reactive. This time I cleansed my face using Thayer's milk cleanser, which is very soothing, and in fact is one of the only non-Korean beauty products in my skincare routine. Then I used my TirTir milky toner. I waited 5-10 minutes, then I applied just a little bit of this Anua Azelaic Acid over my entire face.
I was very surprised that there was no burning or irritation. And also surprised that a few minutes later some of the redness on my cheeks were calmed down--nothing earthshaking but there was a slight difference.
I let that absorb/sit for about 10 minutes and then I applied my Cosrx ceramide booster serum, which is very hydrating, and after letting that absorb for 5 or 10 minutes, I applied my Cosrx snail music to further hydrate. I'll be applying another moisturizer to seal it all in.
I tried it again the next day and had similar results - again, not using my tret the night before or the C the same morning.
This likely, at least at first, won't be a part of my daily routine as I think it could be too strong for my skin along with the other actives I use. For example, I won't use it when I apply my vitamin C until I lower the strength or until my skin is used to the azelaic acid, and I definitely wouldn't use it right before or right after tretinoin. I will do as they suggest and at first use it maybe 2x a week, so on weekends when I don't use my tretinoin or vitamin c, I will use this Anua azelaic acid. After doing this for a couple weeks, I may experiment and start using it more often --perhaps alternating this and my vitamin c since my vitamin c is really strong. As well I may consider lowering my vitamin c strength, and go back to the lower strength tret. (Update: I was out of town so I took a 4-day break from tret and vitamin c and tried this every morning after toning with hypochlorous acid (I let the HA dry completely first), and this didn't irritate my skin at all, and in fact, helped the redness. I am going to find a way to add it to my routine maybe by alternating days using this and vitamin c.)
This is fragrance free, which is a must for me. The consistency is thin but not overly watery - glides on easily. I bought mine on Spring Sale so I'm happy about that.
I will also add that I use Metrocream, and hypochlorous acid spray (Prequel) on my skin a few times a week and that helps the redness as well.
It's an experimental process to find what will be right for my rosacea and aging skin.
I am hoping with regular use my redness will calm even more as I hate the flushing across my cheeks and nose that I've dealt with for many years.
I'm hopeful!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 4, 2026
★★★★★ 4
Works Great but the price is rising!
Anua Azelaic Acid is a very good serum. Both my daughter and I use it regularly. My daughter has severe rosacea, and this has helped calm her skin significantly her redness is almost gone. For me, it has also made a noticeable difference by reducing redness and giving my skin a healthy glow. We both use it at night, and it’s definitely very soothing and calming. The formula is gentle, lightweight, and works really well for sensitive skin without causing irritation.
However, the only downside is the price, it seems to have increased quite a bit recently, which is concerning. I bought a couple during Amazon Prime Day, which worked out really well. For a relatively simple azelaic acid serum, the price increase feels a bit steep.
If the price continues to rise, I may need to look for a more budget-friendly alternative. Otherwise, it’s a really great serum that delivers great results.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 30, 2026
★★★★★ 5
DERM APPROVED!
I am obsessed with this product!! This feels very luxurious on my skin and does not pill like other products I have had in the past. I have noticed a significant improvement in the background redness in my skin. As someone who works in derm and values good ingredients and products that actually work... I can confidently say that this is the best OTC azelaic acid product I have tried out. It sits very nicely under my moisturizer and does not dehydrate my skin like other azelaic acid products have done in the past. HIGHLY RECOMMEND
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 10, 2026
recommand products
Happy Birthday Maria v2 - Personalized Stainless Steel Cross Necklace
59.95
Happy Birthday Jennifer v2 - Personalized Stainless Steel Cross Necklace With Mahogany Style Luxury Box
79.95
Happy Birthday Maria v2 - Personalized Stainless Steel Cross Necklace With Mahogany Style Luxury Box
79.95
Happy Birthday John - Stainless Steel Ball Chain Cross Necklace With Mahogany Style Luxury Box
79.95
Happy Birthday Robert v2 - Personalized Stainless Steel Ball Chain Cross Necklace With Mahogany Style Luxury Box
79.95