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Description
Rat IL-1α ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 200 pg/mL). Then dilute to the following concentrations: 200 pg/mL, 100 pg/mL, 50 pg/mL, 25 pg/mL, 12.5 pg/mL, 6.25 pg/mL, 3.125 pg/mL, and 0 pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 200pg/mL standard working solution into the first EP tube and mix thoroughly to make a 100pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotinylated detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 1 Alpha (IL-1α) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Interleukin 1 Alpha (IL-1α) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Interleukin 1 Alpha ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Interleukin-1α (IL-1α), also known as hematopoietic factor 1, is a cytokine of the interleukin-1 family, encoded by the IL1A gene. Generally, interleukin-1 is responsible for producing inflammation and promoting fever and sepsis. IL-1α inhibitors are currently being developed to disrupt these processes and treat diseases. IL-1α is primarily produced by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It has metabolic, physiological, and hematopoietic activities and plays a central role in regulating immune responses. It binds to the interleukin-1 receptor, which is involved in the activation of tumor necrosis factor-α. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 3.12-200 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.6 ★★★★★
Based on 6 reviews
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Product Reviews
★★★★★ 4
Thoughtful Easter devotional, though the writing style may not resonate with everyone
Format: Paperback, Format: Paperback
As Easter approaches, I was interested in spending some time with this devotional. One thing I appreciated right away is how Paul David Tripp connects passages from across the Bible to the deeper meaning of Easter. The devotional begins with references in the Old Testament and traces the thread of prophecy forward toward the ultimate sacrifice and resurrection. That connection between early scripture and the events of Easter is an important foundation of the Christian story, and the book highlights it well.
What I liked:
Carefully selected Bible passages that connect Old Testament prophecy with the Easter story
A devotional structure that encourages reflection throughout the season
An accessible way to revisit the narrative from prophecy to resurrection
Where it didn’t fully connect for me:
The author’s narrative style may be a matter of personal preference. His writing is often elegant and reflective, but at times it felt somewhat fragmented or incomplete to me. I personally tend to connect more with a slightly more straightforward, conversational style of devotional writing.
That said, this doesn’t mean the book lacks value. Readers who appreciate a more poetic or contemplative tone may find it quite meaningful.
Overall:
Even though the writing style wasn’t a perfect fit for me, this is still a worthwhile Easter devotional. It provides a thoughtful way to reflect on the journey from prophecy to resurrection and would make a good companion for the Easter season.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 8, 2026
★★★★★ 5
Equally deep and approachable devotional
Format: Paperback
This really is an everyday devotional in that it is rich and engaging whether going through it for the Lenten season or any other time of the year. It focuses on the Gospel message from our sin and need for a Savior, through the Lord’s redemptive plan and faithful fulfillment through Jesus. Each daily entry is just two pages, but has a balance of depth and approachability that makes it feel full and spiritually satisfying. The author’s personal stories and way of framing the biblical passages is relatable, and I was immediately drawn into it. I especially like how the devotional begins with the Old Testament scriptures, revealing themes and prophetic pictures that I hadn’t seen before, and then gradually works into the New Testament, even through Revelation! If going through this during Lent, the topics build up the anticipation for Resurrection Sunday and are arranged very meaningfully. The reflection questions are also really good at bringing out deep reflections and a personal response to the biblical passage and thematic focus of the devotional. I’ve made it through most of the devotional at this point, and am thoroughly enjoying it. Highly recommend!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 16, 2026
★★★★★ 5
Love this!
Format: Paperback
I recently received this christian daily devotional book and here’s my quick thoughts on it:
I think that the condition of the book is great and it doesn’t feel cheap or like it would rip very easily. I think this is a good quality paper and material.
I admire this daily devotional book because it is something great to read as soon as you wake up, preparing your day under Christs’ armor. I actually have another daily devotional book which is a year long and it gives encouragement through scripture directly from the word of God. That book is great and it’s amazing reading as soon as I wake up every morning.
This book is nothing short of how good that one is and I just love how this one is structured.
Overall, I highly recommend this every day Christian devotional book to anyone looking for one and even as a gift for someone! I love this!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 12, 2026
★★★★★ 5
A Beautiful, Gospel-Filled Devotional for Every Season
Format: Paperback
I have truly been enjoying reading Everyday Gospel Easter Devotional and applying the teachings to my own life. This devotional has been so helpful in fueling my faith and deepening my understanding of the incredible gift that Jesus gave us all on the cross.
What I really love is that this isn’t something you can only use during Easter. It can absolutely be read and treasured year-round. The lessons are rich, meaningful, and rooted deeply in Scripture.
It begins with teachings from Genesis and ends with Revelation, so you really get to journey through the Bible from beginning to end. I’ve learned so much and gained fresh insight into God’s redemptive plan throughout Scripture.
This devotional is perfect for babes in Christ as well as those who are mature in the faith. It presents biblical truth in a way that is easy to understand, yet still powerful and spiritually enriching.
I would definitely recommend this devotional to anyone wanting to grow deeper in their faith and better understand the Gospel message. It’s a beautiful reminder of the hope we have in Christ.
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Reviewed in the United States on March 3, 2026
★★★★★ 5
Works great, easy to assemble.
Size: 2 Tier-9.5''Wx10.5''Dx9.5''H
I bought this to try and contain our cabinet clutter. It works really well. Was easy to assemble.
I use it on the countertop instead of inside of the cabinets. Looks nice enough to sit out.
Will buy 1 more in the coming days.
The storage areas pull out, makes for easy access.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 16, 2026
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