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Description
Human FCRL3 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an Fc receptor-like protein 3 (FCRL3) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Fc receptor-like protein 3 (FCRL3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Fc receptor-like protein 3 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Fc receptor-like protein 3 (FCRL3) is a protein encoded by the FCRL3 gene. This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains immunoreceptor tyrosine-based activation motifs (ITAMs) and immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic domain and may play a role in regulating the immune system. Mutations in this gene have been associated with rheumatoid arthritis, autoimmune thyroid disease, and systemic lupus erythematosus. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.8 ★★★★★
Based on 20 reviews
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Product Reviews
★★★★★ 5
Mixes matcha well
This is great for mixing matcha due to being able to adjust the speed and make it slower or faster. The stand is a great bonus.
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Reviewed in the United States on May 27, 2026
★★★★★ 5
Rechargeable hand mixed.
Blends very well and fast. Sound is very quiet. Worth the price. Recommend to friends and family. Recharges fast. Very versitatable i.e eggs, frothing and drink mixes. Good power.
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Reviewed in the United States on May 21, 2026
★★★★★ 5
Worth the money.
It’s very nice. Good quality. Not cheap. Handles well. I’m very satisfied!
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Reviewed in the United States on May 27, 2026
★★★★★ 5
One of the best genral-purpose frothers out there, period
For the longest time, I've been looking for what seems like a unicorn -- something about the size of a mini-frother, but stronger than most. Almost a mini-submersion blender, really. And I still haven't found it. But the Maestri House frothers are about the closest thing I have found, which gives me hope that my unicorn might really exist someday.
Unlike cheaper frothers that almost always warn you off from actually washing them, Maestri House makes theirs waterproof. Seriously, you can froth sloppy and get it all up in the whisk end of this, then rinse the whole thing under the faucet if you want to. I really don't recommend that for the speed ring at the top of this one, as a precaution and because it should never be necessary, but you can if you have to.
Despite what I believe is cheaping out on the battery at just 1200mAh, it really can last a while, especially if you use it just once or twice a day. That it recharges using a USB-C connector is definitely a bonus.
The speed ring at the top is great feature, overall. It turns on at a slow speed and lets you crank it all the way up to a speed that I still haven't found a good use for. This provides a lot of flexibility, from gentle mixing to full-on danger frothing.
The included whisks can all be pretty handy. The whisk-whisk is good for things like two or three whole eggs, and the frother-whisk is good for general-purpose frothing. I really haven't figured out what the hook-whisk is for, because this isn't really made for handling any kind of dough, but it might work well for medium-to-thin batters.
Generally speaking, I can easily recommend Maestri House frothers over just about any other handheld frother you're going to find. For completeness, though, there are a few things that I think could be improved upon:
- The battery. Once you're into the $25+ range, I would expect a a high-current 2000mAh battery, for longevity and torque.
- The speed ring is flexible, but depending on your dexterity and grip it can be tricky to operate when dialing in just the right speed, with just one hand. And there's no "Off" button, so you have to wind it down to "Off". For some reason I often end up spinning it the other way and making it go faster instead. Muscle memory should take over eventually, but still. A dedicated On/Off button would be handy.
- For as beefy as this device feels, it doesn't handle thick liquids as well as I expected. The torque for basic mixing and frothing tasks is more than enough, but get this into thicker liquids or even four+ eggs, and it starts to bog down.
- Like most frothers of this type, the balance of the whisks is a variable thing. If a whisk shaft gets even a little bent this thing will shake, at least when started at slow speed. Things usually settle out at higher speeds, but I'd really like to see the shaft of the whisks made thicker and more rigid so they don't bend so easily.
- The frother whisk is good for what it is, and will quickly make great, basic foam. But unless you have supreme technique, it probably won't be good for any kind of latte art. This isn't a big problem in my mind, just something to be aware of.
- The stand is okay at best, and unstable at worst. It's just a bit short for some whisks, so some of them can bottom out on the counter and make it unstable. Bending the holder at the top back just a bit with some good pliers, so the whisks are tilted up and away from the bottom, can help. I'll probably end up designing a 3D printed stand to hold this more security, as well as store the extra whisks.
- I'm really not a fan of the textured finish on this model. It feels good enough, and the color is okay, but there are obvious seams on each side that, in my opinion, take away from the aesthetic. My call would be that whatever finish might be on the body, it should be seamless around the entire cylinder.
I want to be clear, that none of the comments above are intended to warn you off of this if you're looking for a very good, well made frother. I doubt that you can find anything better! I only mention them because nothing is perfect, and to temper the reader's expectations.
This brand, if not this specific model of frother, is almost certainly everything that you are looking for. If there's anything you don't like about this particular model on paper, I urge you to consider literally any other handheld frother they make.
It only falls short compared to my unicorn device, something I don't ever expect to see from anyone else, but really believe that Maestri House could deliver if they think there's a market.
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Reviewed in the United States on March 12, 2026
★★★★★ 4
It is an upgrade from PRODUKT
There's a lot to like with this frother. First off, it gets super-fast. It has good power but struggles a bit through thicker liquids at high speeds. I really got it exclusively for frothing milk and making really smooth chocolate milk. For those things it works well.
I like the stand. I just keep a napkin under it so it will drip dry after rinsing. It is easy to clean because you can just run it on about medium under running water. I've been using it for about 3 weeks now, probably once every other day, and I'm still on the first charge. To me that is excellent battery life.
It spins true, even at high speed. The key is to treat it well and probably make sure to use the stand instead of laying it on the counter. Because of the high RPM, any imbalance or bend at all is really going to be exaggerated.
The only thing I am not crazy about is the control. I don't like that I have to twist it off while in the liquid. I wish there was just a switch along with the adjustment ring.
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Reviewed in the United States on March 28, 2026
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