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Description
Mouse ANXA9 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an Annexin A9 (ANXA9) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the Annexin A9 (ANXA9) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Annexin A9 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Annexin A9 (ANX/A9) is a protein encoded by the ANXA9 gene. Annexins belong to a family of calcium-dependent phospholipid-binding proteins. Members of the annexin family contain four internal repeating domains, each of which includes a type II calcium-binding site. This calcium-binding site is required for annexin aggregation and cooperative binding to anionic phospholipids and extracellular matrix proteins. This gene encodes a diverged member of the annexin family in which all four homologous type II calcium-binding sites within the conserved tetrad core contain amino acid substitutions, resulting in loss of function. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.7 ★★★★★
Based on 23 reviews
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Product Reviews
★★★★★ 4
Sturdy Folding Room Divider with Good Privacy Coverage and Modern Look
Color: Black, Size: 4 Panel-88''
I picked this room divider up to help separate space in a room without needing permanent walls, and overall it works very well for creating privacy while still looking clean and modern.
The size is one of the biggest advantages. At 6 feet tall and roughly 88 inches wide, it creates a noticeable visual separation and works well for apartments, dorms, offices, studios, shared rooms, or content creation setups where you want to hide clutter or define different areas.
The black fabric and frame design give it a simple modern appearance that blends easily into most spaces without looking overly decorative or outdated. It has a clean minimalist look that works especially well in offices, bedrooms, gaming rooms, or studio environments.
Assembly was fairly straightforward overall. Once fully put together, the divider feels reasonably stable for normal indoor use. The folding panel design also makes it flexible enough to angle different ways depending on the room layout.
The portability is another strong point. Since it folds down, it is much easier to move or reposition compared to permanent partitions or heavier wooden dividers. That makes it practical for temporary privacy setups or changing room layouts frequently.
Privacy performance is solid visually. It blocks sightlines well enough for changing areas, workspaces, video backgrounds, or separating sections of a room. The fabric also softens the space slightly instead of feeling harsh or industrial.
One thing worth noting is that while it provides visual privacy, it is not soundproof. It helps define space and reduce some openness, but conversations and noise will still pass through normally.
The material quality feels decent for the price range. The frame is lightweight but functional, though I would still avoid rough handling or constant aggressive repositioning over time.
Value for money is good considering the size, flexibility, and convenience. Similar room dividers can become expensive quickly, especially for taller multi-panel setups.
Overall, this is best for anyone needing an affordable flexible way to create separation and privacy in shared or open spaces. It works especially well for apartments, dorms, offices, studios, remote work setups, or content creators wanting a cleaner background without permanent construction.
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Reviewed in the United States on May 8, 2026
★★★★★ 5
Looks nice but it's not very stylish, works as intended, happy with purchase
Color: Black, Size: 4 Panel-88''
This 6 ft room divider is exactly what I needed and works great! It was very easy to set up and took only a few minutes to assemble. I was pleasantly surprised by how lightweight it is, and it folds up easily for storage when not in use.
The fabric-style panels look nice and provide good privacy, and the included clips help keep everything neatly in place. It’s a simple but effective design that does what it’s supposed to do.
Because it’s lightweight, it may need a bit of extra support depending on where you place it—I used small weights on the base, and that completely solved the stability issue.
Overall, I’m very happy with this purchase. It’s practical, easy to use, and a great value for the price. I would definitely recommend it! Industrial
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Reviewed in the United States on May 22, 2026
★★★★★ 3
Assembly is harder than it should be; you get what you paid for
Color: Black, Size: 4 Panel-88''
Review of SUNALLY Room Divider 6FT Folding Privacy Screens, 4 Panel Fabric Divider for Room Separation, 88" W Freestanding Portable Wall Dividers Screen for Home Dorm Studio Office, Black
I hate when I have to leave products a not so good review...
Assembly is harder than it should be, mainly because there shouldn't BE any assembly. I have ordered several room divider privacy screens over the last couple of years and not one of them needed any kind of assembly. With that aside, it STILL was a pain in the sphincter to assemble. One of the "legs" just sits on top of a foot plate - it's not screwed in to anything.
It's kind of flimsy and the base plates don't have anything on them to not scuff up the floor.
With all that said, though, it does stand up and will block out what I need to block out. At the less-than-$50 price-point, it's kind of what should be expected. If I had purchased this outright, I would have considered sending it back and getting a higher quality. But if you only have $50 to spare and don't mind assembling things, it could be good for you.
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Reviewed in the United States on April 28, 2026
★★★★★ 5
Great buy
Color: Black, Size: 4 Panel-88''
This room divider is exactly what I needed. When you have 2 kids sharing a room and don’t want to spend a lot of money to divide their space this is what you can use. Believe it or not they are much happier now. This came in a small box which scared me but once I was able to get this up I was very pleased. It was easy to put together. The fabric is pretty good quality, it’s stable and stands up fine. Good value and worth it to have peace and quiet in the house.
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Reviewed in the United States on May 18, 2026
★★★★★ 1
Junk
Color: Black, Size: 4 Panel-88'', Color: Black, Size: 4 Panel-88''
Horrible. The parts are not right. It won’t fit together or disassemble and can’t return it like this. Parts won’t fit properly and missing holes for screws. Also, can’t get through to a person for any assistance. Picture shows the hole and then the lack of a hole where a screw belongs.
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Reviewed in the United States on May 13, 2026