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Description
UniOne® TR-FRET Human IL10/IL10RA Binding KitProduct Specification Host Human Stability & Storage 80 Background This kit utilizes HomogeneousTime Resolved Fluorescence (TR FRET) technology to measure the interactionbetween IL10A and IL10RA. This method enables simple and rapid high throughputscreening of inhibitors and antibody blockers. As illustrated, the interactionbetween IL10A and IL10RA is detected using an Eu labeled Anti Tag1 antibody(TR FRET donor) and an Acceptor labeled IL10A protein
Product Specification
| Host | Human |
| Stability & Storage | -80℃ |
Background
This kit utilizes HomogeneousTime-Resolved Fluorescence (TR-FRET) technology to measure the interactionbetween IL10A and IL10RA. This method enables simple and rapid high-throughputscreening of inhibitors and antibody blockers.
As illustrated, the interactionbetween IL10A and IL10RA is detected using an Eu-labeled Anti-Tag1 antibody(TR-FRET donor) and an Acceptor-labeled IL10A protein (TR-FRET acceptor). Thebinding of IL10A to IL10RA brings the donor antibody and the acceptor proteininto close proximity. Excitation of the donor antibody then triggersFluorescence Resonance Energy Transfer (FRET) to the acceptor antibody,resulting in the emission of a specific signal at 665nm. The intensity of thisspecific signal is proportional to the extent of the IL10A and IL10RAinteraction. This homogeneous assay is simple to perform and requires nowashing steps.
Components
Kit Components】
Component |
Concentration |
500 TESTS |
2500 TESTS |
10000 TESTS |
Storage Temp. |
|---|---|---|---|---|---|
IL10A-01A protein |
100× |
25μL |
125μL |
500μL |
-80°C |
Tag1- IL10 RA protein |
100× |
40μL |
200μL |
800μL |
-80°C |
Anti-Tag1 Eu dye antibody |
50× |
50μL |
250μL |
1000μL |
-80°C |
Detection buffer |
10× |
2mL |
10mL |
40mL |
-80°C |
*Reagents should be protected from repeated freeze-thaw cycles. It is recommended to aliquot upon first thaw and store according to the specified conditions. The shelf life is one year.
Shipping Notes
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
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