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Description
Mouse ATP1b4 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse ATPase, Na+/K+ Transporting Beta 4 Polypeptide ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | The protein ATP1B4 (ATP1b4), also known as ATPase Na+/K+ transporting family member β4, is encoded by the ATP1b4 gene. It functions as a transcriptional regulator during muscle development through interaction with SNW1. It has lost its ancestral function as the β subunit of the Na,K-ATPase. Diseases associated with it include mannoses, Beta A, lysosomal, and thyrotoxic periodic paralysis. Pathways involved include GPCR signaling and the transcriptional activity of the SMAD2/SMAD3-SMAD4 heterotrimer. Two transcript variants encoding distinct isoforms have been identified for this gene. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.6 ★★★★★
Based on 17 reviews
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Product Reviews
★★★★★ 5
Trimmer
Works great
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 26, 2025
★★★★★ 5
Good for household use.Even my wife likes it!!
Good product.I had one previously that lasted for years.I like the lightweight and power is more than adequate!
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Reviewed in the United States on June 7, 2025
★★★★★ 5
Quick, powerful, and easy to use
Style: 2 x 2.0Ah
When my wife and I bought our latest home, I got bitten by the battery-powered lawn equipment bug. Because our lawn is a lot smaller and flatter than our last home, I refrained from having landscapers regularly maintain this new lawn. This means I now have every piece of equipment one could possibly want to keep our yard looking beautiful
Before purchasing this last item for our lawn, I was using a weed whacker to do the edging. This was tedious and not very precise. Because I have all Greenworks-branded equipment, this edger was a no-brainer.
Even though most of our battery-operated lawn equipment uses 60v batteries, I opted for this smaller dual-battery setup to save a little weight and money. Because of the small battery sizes, the edger is perfectly balanced and not one bit unwieldy.
I am able to quickly clear all of our sidewalk, lanai, and driveway surface edges within minutes.
Up to this point, I had never used an edger before. It literally took less than 30 seconds to get it buzzing correctly along the surface edges of our property. If not trying to do a large commercial area, this edger will probably work for most residential applications. Quickly and easily.
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Reviewed in the United States on July 24, 2025
★★★★★ 5
It's a beast and you can add attachments to, like a brush cutter head.
Style: 16" (2 x 4Ah)
This thing is a beast of a battery powered machine. I wouldn't put it completely up against my gas. It is much better than the Greenworks 24V 13-Inch Brushless String Trimmer (Gen 2), 4.0Ah USB Battery and Charger Included that I had purchased this time last year. I have several gas so I only really needed a light duty one to do the fence line and around the house. The main issue with the 13 inch was that the auto feed bump mechanism was worthless. It also uses very thin lines so very quickly you go from 13 inches to 6 to 8. and the bump feed does not work even with greenworks replacement spools. You get twice the batteries and a dual rapid charger for $50 more. You couldn't even buy the battery for that much.
onto this thing. Love the dual string heavy-duty string mechanism. Love the fact that you can remove the trimmer head and add an attachment. I have attachments from my gas Trimmers and they work perfectly. My brush head attachment utterly devastated thick bramble from an overgrown lot. The best part is it took me two hours to get my stored gas trimmers to run properly which I don't have to do with a battery operated device. No gas flying that deteriorated or primer bubbles that have burst followed spark plugs Stale gas . Just throw the batteries on and you're off.
Nothing long-term to report, only had it a week. Used it enough to run down completely six sets of batteries.
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Reviewed in the United States on April 30, 2026
★★★★★ 4
Works well, but battery life is lacking
Style: 15" (2 x 2Ah)
This is a decent trimmer and gets the job done almost as well as a gas model. It has decent power (could use a little more) and is plenty light.
However, the battery life stinks. One of the two batteries has already decided not to charge. It plugs in, but the charger does not blink or charge it. The terminals are clean.
Of course, this happened just outside the return/support window, meaning I now have to buy a new battery just to use the trimmer. That's about half the price of the trimmer; if the other one dies, too, I may as well buy a brand new gas trimmer for the price and have the thing last for a decade or two like my last one, instead of a couple years like this one.
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Reviewed in the United States on September 6, 2023