Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 28 - Sep 2
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human CPVL ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. 3. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against carboxypeptidase Vitellogenic Like (CPVL). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of carboxypeptidase Vitellogenic Like (CPVL) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Carboxypeptidase Vitellogenic Like ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Phosphatidylcholine-producing carboxypeptidase-like protein (CPVL) is an enzyme encoded by the CPVL gene. The CPVL gene is primarily expressed in monocytes and macrophages, localizing to the endoplasmic reticulum and endosome/lysosome compartments. The distribution of CPVL suggests that it may be involved in antigen processing and secretion pathways. In addition to tissues rich in macrophages, high levels of CPVL mRNA are also expressed in the heart and kidney. This enzyme is similar to the carboxypeptidases CATHA and SCPEP1. The CPVL gene is located on chromosome 7p15.1 and consists of 14 exons. At least two alternatively spliced transcripts encoding the same protein have been observed. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.6 ★★★★★
Based on 14 reviews
Sort
Product Reviews
★★★★★ 5
Incredible…
This stuff is incredible. I used to take a psyllium supplement semi regularly but would always stop after a week or so of getting tired of it. This stevia version of Metamucil is better than any fiber I’ve ever taken. Tastes great, dissolves immediately, works incredibly well, and doesn’t get old or repetitive like other versions. Flavorful but not overly sweet. It’s amazing what it does to your poops too. Unbelievable.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 29, 2022
★★★★★ 4
Not real sweet, but no sugar
I have been taking Metamucil with real sugar regularly for 20+ years. I became concerned about the amount of sugar I was consuming daily and cannot tolerate aspartame. So, I jumped to buy this when I saw Stevia. I quickly noticed that this version is not sweet. It’s sort of bitter. Also, the texture is different due to not having sugar granules mixed in. It also converts to a gel like texture quicker. I later noticed you use less of this product per serving than the one with sugar (read the label!). I have found the best way to take this is to very quickly (seconds) consume the serving after adding water. Don’t wait too long. I down it like taking a shot out of a shot glass followed quickly by a glass of water. After a while, I got used to the flavor. Overall, I am pleased I am consuming much less sugar and really don’t notice the change anymore.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 2, 2022
★★★★★ 5
This product is very important to your well being.
The Metamucil is everything I hoped for. If you’re concerned about your sugar intake then I suggest the one with Stevia. Yes the one with natural sugar tastes the best but once you graduate to 3 times a day that’s a lot of sugar. I would follow the directions. Start with once a day then after 3 or 4 days you’ll find twice a day works nice. I’m on my 4th week so a few days ago I started 3 times. I’m very pleased with the way I feel. Think about it, we take care of our cars better than ourselves sometimes. It’s so important to take care of your digestive system. Have a good day. 😁
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 23, 2021
★★★★★ 5
Great flavor, less calories than real sugar, thickens very quickly.
I usually get the real sugar version of this rather than this one with Stevia. I have it every morning just to supplement my diet with fiber and the version with stevia is only 30 calories vs 80 for the one with real sugar. I mix it with unflavored protein powder in a shaker cup and this one thickens very quickly. So quickly that I add water after every quarter of the container that I drink and re-shake. Otherwise it gets so think it won’t even come out by the time I’m halfway through. Once I figured that out it’s no big deal but makes it so that I won’t take it in the car to drink on the go.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 26, 2019
★★★★★ 5
IBS C must have!
I have been suffering with OBS C for a very long time now and my doctor through Amazon Care (if you haven't downloaded that app yet, do it now) recommend that I take magnesium and the Align probiotic with breakfast and Metamucil in the evening before bed. I am finally feeling better and have not had any issues using the restroom! I recommend for sure. The taste is meh but I would rather down something that doesn't taste great as long as I feel better anyway!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 2, 2022
recommand products
Portrait of J.P. Morgan, 1837 - 1913. Impressionistic painting of a man with a mustache.
24.00
Portrait of Joseph Lister, 1827 - 1912. Impressionistic painting of a man in a suit.
24.00
98-03 Jaguar X308 XJR XJ8 Fuel Filler Gas Tank Door Flap Lid Gray OEM
44.86
SANYO 610 292 4848 Compatible Module with Original Bulb, Applicabler Projectors: PLC-EF32 , Alt. LampPart Number: POA-LMP39
358.70
14-24 Jaguar X152 F-Type Gas Fuel Pump Sending Unit w/ Level Sensor OEM
112.05