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Description
Vimentin Recombinant Rabbit mAb,PBS Only (SDT-029-120)Product Specification Host Rabbit Antigen Vimentin Synonyms VIM Location Cytoplasm, Cytoskeleton, Cell membrane Accession P08670 Clone Number SDT 029 120 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P, ICC, IP, ICFCM Reactivity Hu, Ms, Rt Predicted Reactivity Bovine, Monkey, Chimpanzee, Pig, Chicken, Hamster Purification Protein A Concentration 0. 25 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40%
Product Specification
| Host | Rabbit |
| Antigen | Vimentin |
| Synonyms | VIM |
| Location | Cytoplasm, Cytoskeleton, Cell membrane |
| Accession | P08670 |
| Clone Number | SDT-029-120 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IP, ICFCM |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Bovine, Monkey, Chimpanzee, Pig, Chicken, Hamster |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500 | |
| IP | 1:25 | |
| IHC | 1:1000 | |
| ICC | 1:500 |
Background
Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all animal cells as well as bacteria. Intermediate filaments, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression. It can be used as a differential diagnosis between epithelial tumors and mesenchymal tumors, as well as for endometrial adenocarcinoma and cervical adenocarcinoma.
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