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Description
S-RMab® IGJ Recombinant Rabbit mAb (S-342-48)Product Specification Host Rabbit Antigen IGJ Synonyms Immunoglobulin J chain, Joining chain of multimeric IgA and IgM, JCHAIN, IGCJ Immunogen Recombinant Protein Location Secreted Accession P01591 Clone Number S 342 48 Antibody Type Recombinant mAb Application WB, IHC P, IP Reactivity Hu Purification Protein A Concentration 0. 25 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05%BSA, 0. 03% Proclin 300
Product Specification
| Host | Rabbit |
| Antigen | IGJ |
| Synonyms | Immunoglobulin J chain, Joining chain of multimeric IgA and IgM, JCHAIN, IGCJ |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | P01591 |
| Clone Number | S-342-48 |
| Antibody Type | Recombinant mAb |
| Application | WB, IHC-P, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500 | |
| IP | 1:25 | |
| IHC-P | 1:4000 |
Background
The Joining (J) chain is a protein component that links monomers of antibodies IgM and IgA to form polymeric antibodies capable of secretion. The J chain regulates the multimerization of IgM and IgA in mammals. When expressed in cells, it favors the formation of a pentameric IgM and an IgA dimer. The J chain is incorporated late in the formation of IgM polymers and thermodynamically favors the formation of pentamers as opposed to hexamers. The J chain facilitates IgA dimerization by linking two monomer secretory tails. Structurally, the J chain joins two antibody monomers asymmetrically by forming intermolecular disulfide bonds and bringing hydrophobic β-sandwiches on each molecule together.
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Exchange/Return Notes
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