Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 27 - Sep 1
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human LMNA ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Gently wash adherent cells with pre-chilled PBS, then trypsinize and collect the cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with pre-chilled PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Collect the supernatant for analysis, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a lamin A/C (LMNA) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of lamin A/C (LMNA) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Lamin A/C ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Lamin A/C, encoded by LMNA, belongs to the lamin family of proteins. LMNA-associated diseases include Hutchinson-Gilford Progeria Syndrome and Cardiomyopathy, Dilated, 1A. Pathways associated with this gene include apoptotic cleavage of cellular proteins and SLBP-independent mature mRNA trafficking. Gene Ontology (GO) annotations associated with this gene include structure, molecular activity, and activity. An important homolog of this gene is LMNB2. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.8 ★★★★★
Based on 26 reviews
Sort
Product Reviews
★★★★★ 5
52, Post-Menopausal Dry Skin — This Is My Barrier Emergency Product
Size: 1.35 Fl Oz (Pack of 1), Style: Cicaplast Baume B5 Balm, Size: 1.35 Fl Oz (Pack of 1), Style: Cicaplast Baume B5 Balm
I’m 52 with post-menopausal, mature dry skin, and this is not something I use casually or every day — it’s what I reach for when my skin barrier is compromised and needs real help. When my skin is irritated, over-exfoliated, reacting to actives, or feeling tight and inflamed, I don’t want glow or anti-aging claims. I want calm, protection, and repair. This balm delivers exactly that.
The texture is rich and occlusive, but not greasy or suffocating. It melts into the skin and immediately takes the edge off irritation — that tight, uncomfortable feeling just stops. Within minutes, redness looks calmer, dryness is softened, and my skin feels protected rather than coated. It creates a breathable barrier that actually stays put overnight, which is especially important on mature skin that loses moisture quickly.
What I appreciate most is that this works with my existing routine instead of competing with it. I use growth factors, peptides, and treatment serums regularly, and when my skin needs a reset, this seals everything in without stinging or interfering. I’ve used it after retinoids, during periods of sensitivity, and when my skin simply feels fragile — and it consistently helps restore balance.
That said, if you have very oily skin or are looking for a lightweight daytime moisturizer or a dewy “glass skin” finish, this probably isn’t the product for you. This is a true treatment balm, designed for barrier repair rather than cosmetic glow.
This is not a glow cream, a makeup primer, or a lightweight moisturizer, and that’s exactly why it’s so effective. It’s a true barrier balm. On my dry, post-menopausal skin, it reduces flaking, minimizes irritation, and helps my skin recover faster so I can get back to my normal routine without setbacks.
With so many skincare products making big promises, this one quietly does its job — and does it extremely well. If you have mature, dry, or reactive skin and need something reliable for barrier repair, this earns its place. It’s not exciting, it’s not trendy — it’s just very, very effective.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 6, 2026
★★★★★ 5
Lightweight SPF that layers well under makeup
Size: 5 Fl Oz (Pack of 1)
This sunscreen feels much lighter than many high-SPF formulas I’ve tried and blends into the skin without leaving a heavy white cast. The texture spreads easily and gives a hydrated finish without feeling greasy, which makes it comfortable for everyday wear on both the face and body. I also liked that it layered well under makeup without pilling.
I used this during long outdoor days and it held up well while still feeling comfortable on the skin. The SPF 60 protection is reassuring, especially for travel, beach days, festivals, or daily sun exposure. Overall, this is a reliable sunscreen option if you want strong protection with a more wearable, skincare-like texture.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 9, 2026
★★★★★ 5
A Must-Have for Sensitive Skin
Size: 5 Fl Oz (Pack of 1)
recently tried La Roche-Posay sunscreen and I’m really impressed! 🌞 The texture is lightweight, non-greasy, and absorbs quickly without leaving a white cast, which is a big plus for daily wear. It feels gentle on my skin and doesn’t clog pores, making it perfect for sensitive or acne-prone skin. I also love that it provides strong, broad-spectrum protection while still feeling breathable. Overall, it’s become my go-to sunscreen—I actually enjoy applying it every morning because it feels more like skincare than sunscreen. Definitely worth it!
I would be honored if you would consider me to receive a PR package. I’d love the opportunity to try more of your products and create authentic content that highlights the benefits of La Roche-Posay skincare.
Thank you so much for your time and consideration!
Warm regards,
Andrea Mohanlall
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 4, 2025
★★★★★ 5
Goes on smooth with no white cast.
Size: 3 Fl Oz (Pack of 1)
This is excellent and disappears on the skin. Smooth as silk.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 10, 2026
★★★★★ 4
It feels very good on the skin.
Size: 3 Fl Oz (Pack of 1)
I’ve been using this sunscreen for about a week now, and honestly, I’ve liked it even more than I expected. The skin on my face feels much softer and healthier, and one thing I’ve definitely noticed is that my dark circles are gradually looking a bit lighter and less tired. I love it because it doesn’t feel heavy, absorbs quickly, and doesn’t leave a greasy residue.
I also like that it works well under makeup or just on its own for everyday use. For now, I’m giving it 4 stars because I haven’t been using it for very long yet and want to see more results, but so far, I definitely feel a difference in my skin. If I continue to see more positive changes, I’ll come back to update my review.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 30, 2026
recommand products
1 1/2" Black Cable Clamp , 1/2" wide 250/bag, Part #CC5-1 1/2-0H
33.95
2023 Reading Fightins Top 10 Prospects Mick Abel
13.00
NOBL TR37 / I9 Hydra Front Wheel - 29" - 15x110mm - 6-Bolt - Black Decals
288.50
HST-A3-1/4-330-Blk 3:1 Polyolefin Dual Wall/Adhesive Lined Heat Shrink Tubing Nominal Diameter: 1/4" Spool Size: 330 Feet
117.31
Wiring Duct Cover Part #TYD2CPG6
139.50