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Description
Mouse GLNS ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a competitive enzyme-linked immunosorbent assay (ELISA). Samples, standards, biotin-labeled antibodies, and HRP enzyme conjugates are added sequentially to microwells pre-coated with universal species glutaminase (GLNS) antigens. After incubation and washing, the cells are developed using the substrate TMB. TMB is converted to blue under the catalysis of peroxidase (HRP) and to the final yellow under the action of acid. The intensity of the color is negatively correlated with the universal species glutaminase (GLNS) in the sample. The absorbance (OD value) is measured at a wavelength of 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Glutaminase ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Glutaminase (EC 3.5.1.2, glutaminase I, L-glutaminase, glutamine aminohydrolase) is an aminohydrolase that converts glutamine to glutamate. It has tissue-specific isoenzymes and plays an important role in glial cells. It is expressed and active in hepatic pericytes, where it generates NH3 (ammonia) for urea synthesis, similar to glutamate dehydrogenase. It is also expressed in renal tubular epithelial cells, where the generated ammonia is excreted as ammonium ions. Ammonium excretion is a key mechanism of renal acid-base regulation. During chronic acidosis, glutaminase is induced in the kidneys, resulting in increased ammonium excretion. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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Perfect Facial Wipes
Style: Original, Size: 25 Count (Pack of 2)
These are great for washing makeup off if, like me, you cannot use soap on your face. They are convenient to use; just pull from the package. They are moist and leave your skin clean and smelling fresh. These wipes do not irritate my skin or leave an oily residue. I have used them for year and always have a pack in my makeup area.
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Reviewed in the United States on March 26, 2026
★★★★★ 5
My Go-To Makeup Wipes
Style: Original, Size: 25 Count (Pack of 2), Style: Original, Size: 25 Count (Pack of 2)
These have been my go-to makeup wipes for years. Ever since I discovered them, I keep repurchasing them because they work so well.
They do a great job of removing the bulk of my makeup before I wash my face, including eye makeup, without needing a lot of rubbing. I also really like that they don’t leave behind a greasy residue or make my skin feel dry afterward.
The wipes feel gentle on the skin while still being effective, which is important for everyday use.
I also appreciate that the cloth is plant-based and compostable, which feels like a better option compared to regular wipes. The twin pack is also convenient and a good value.
Overall, a reliable staple in my skincare routine and one of the few makeup wipes I consistently come back to.
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Reviewed in the United States on May 8, 2026
★★★★★ 5
Amazing Pimple Patches - Actually Work!
Size: 36 Count (Pack of 1)
Effectiveness: These Mighty Patch Original hydrocolloid patches have been absolutely incredible for treating breakouts quickly and effectively without harsh chemicals or painful extraction. The patches work remarkably well overnight, drawing out impurities and reducing the size and inflammation of pimples by morning. I love how they protect blemishes from picking and touching, which prevents further irritation and scarring. The clear design makes them virtually invisible during wear, so I can use them during the day without feeling self-conscious. The patches stick securely without being painful to remove, and they genuinely speed up the healing process compared to leaving breakouts untreated.
Quality: Hero Cosmetics has created an exceptional product with these hydrocolloid patches that clearly demonstrates superior manufacturing quality. The adhesive is perfectly balanced - strong enough to stay in place through sleep and daily activities but gentle enough to remove without skin irritation. The hydrocolloid material is high-grade and effectively draws out fluid and impurities without over-drying the surrounding skin. The patches maintain their integrity throughout wear and don't fall apart or lose effectiveness. The sterile packaging ensures product safety and hygiene, which is essential for treating open or inflamed skin.
Value for Money: These pimple patches provide outstanding value when you consider how effectively they treat breakouts while preventing scarring and further damage from picking. The cost per patch is reasonable considering the dramatic results they deliver, often clearing blemishes faster than expensive acne treatments. The prevention of scarring alone makes these worth far more than their price, as treating acne scars is much more expensive and time-consuming than preventing them. The convenience and effectiveness make these far more cost-effective than multiple skincare products or dermatologist visits for individual breakouts.
These patches have become essential in my skincare routine and consistently deliver impressive results. Highly recommend for anyone dealing with occasional or persistent breakouts who wants fast, effective treatment!
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Reviewed in the United States on November 28, 2025
★★★★★ 5
The best patches!
Size: 36 Count (Pack of 1)
These work amazingly well! I just stick one on before bed and by morning the pimple is noticeably flatter and less red. It really does what it says without any picking or irritation.
They stay on all night even if I toss and turn, which I was surprised by, and they don’t feel uncomfortable at all while sleeping. I also like that they’re thin and kind of blend into the skin, so you don’t really notice them if you wear them during the day either. Super simple, effective, and way less stressful than dealing with breakouts the usual way. They fit well and the size is great, really absorbs all the yuck from a pimple. A great value for money!
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Reviewed in the United States on May 4, 2026
★★★★★ 5
Works Overnight—A Must‑Have for Surprise Breakouts
Size: 36 Count (Pack of 1)
I’ve tried a lot of pimple patches over the years, and the Mighty Patch Original truly lives up to the hype. These patches are incredibly easy to use and work best overnight—just apply to clean, dry skin and let them do their thing.
By morning, whiteheads are noticeably smaller (sometimes completely gone), redness is reduced, and the gunk is visibly absorbed into the patch. I love that they’re thin, clear, and stay put all night without irritation. They also prevent me from touching or picking at my skin, which is a huge bonus.
The 36-count pack is a great value, and I always keep these on hand for surprise breakouts before an important day. Simple, effective, and gentle—this is one skincare product I consistently repurchase. Highly recommend!
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Reviewed in the United States on April 29, 2026