Porcine IL-6 ELISA Kit

Detection Principle:

This kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. The specific anti-porcine IL-6 antibody was pre coated on a high affinity microplate. Standards, samples to be tested and biotinylated detection antibodies are added into the wells of enzyme plate. After incubation, IL-6 in the samples combines with solid-phase antibody and detection antibody to form immune complexes. After washing to remove unbound material, horseradish peroxidase labeled streptavidin HRP was added. After washing, the chromogenic substrate was added to avoid light for color development. Stop the reaction by adding stop solution, and determine the absorbance value at 450 nm wavelength (refer to the correction wavelength of 540nm or 570nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

pre coated 96 well plate, standard, anti-porcine IL-6 detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions

Sensitivity: 1.8  Pg/ml

Detection Range: 125 - 8000 pg/ml

Recovery Range: 70-114%

Storage Method: 2-8 ℃

Standard Curve 

Background:

Interleukin-6 (IL-6)Is a α Helical structure, 22-28kd phosphorylation and different degrees of glycosylation of multifunctional cytokines, which play an important role in the acute phase of disease reaction, inflammation, hematopoiesis, bone metabolism and cancer deterioration. Mature mouse IL-6 has 187 amino acids and has 42% and 85% homology with human and rat IL-6, respectively. The alternative splicing of IL-6 generates a variety of isoforms, some of which show antagonistic properties. Cells known to express IL-6 include cd8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelin), sympathetic neurons, cerebral cortical neurons, adrenal medullary chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cellsFetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islets beta; Cells. IL-6 production is usually under the control of glucocorticoids, catecholamines and secondary sex steroids, and is generally associated with cell activation. IL-6 in the blood of normal people is in the range of 1 pg/ml, which increases slightly during menstruation, moderately in the middle and late stages of some cancers, and significantly after major surgery.

IL-6 triggers cell signals through cell surface receptors, which are heterodimeric complexes composed of ligand binding subunit (IL-6 receptor) and signal transduction subunit gp130. IL-6 binds to the IL-6 receptor, triggering the binding of IL-6 receptor to gp130 and the dimerization of gp130. Gp130 is also a component of CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM receptors. Soluble IL-6 receptor is produced by alternative splicing and protease cleavage. Through the trans signal transduction mechanism, the complex of soluble IL-6 and IL-6 receptor can trigger the response of cells lacking IL-6 receptor on the surface but expressing gp130. The expression of IL-6 receptor is mainly limited to hepatocytes, monocytes, lymphocytes and resting lymphocytes. Due to the wide expression of gp130 molecule, trans signal transduction realizes the response of a wider range of cell types to IL-6. The soluble gp130 spliceosome prevented the trans signal transduction of il-6/il-6r, but could not prevent the signal transduction of other cytokines using gp130 molecule as a co receptor.

IL-6 and tumor necrosis factor alpha; (tnf-α)Together with IL-1, the acute inflammatory response caused by IL-1 plays an almost unique role in fever and liver acute inflammatory response. It also plays an important role in the transformation from acute inflammation to acquired immunity or chronic inflammatory diseases. Dysregulation of IL-6 can promote chronic inflammation, such as obesity, insulin resistance, inflammatory bowel disease, inflammatory arthritis and sepsis, often involving the trans signal transduction of IL-6. In transforming growth factor TGF - β In the presence of IL-6, IL-6 plays an important role in the differentiation of naive T cell phase Th17 inflammatory cells. IL-6 regulates bone resorption and promotes the activity of Th17 inflammatory cells, which is the main factor causing inflammatory joint injury in rheumatoid arthritis. IL-6 is involved in the formation and instability of atherosclerotic plaques. However, IL-6 also has anti-inflammatory effects. For example, skeletal muscle secretes IL-6 during physical exercise. As a growth factor of hematopoietic stem cells, it promotes hematopoiesis, induces B cells to mature into plasma cells, and the immortality of multiple myeloma cells. IL-6 also promotes, but may not initiate, other inflammation related cancers, such as colitis related cancers.