SKU: 7971953670

Rat IL-6 High Sensitivity ELISA Kit

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Description

Rat IL-6 High Sensitivity ELISA KitProduct Specification protein IL 6 Species Reactivity Rat Source Rat Description Detection principle: This kit uses double antibody sandwich ELISA Technologies. Specificity anti Rat IL 6 capture antibody Pre coated on high affinity enzyme plates. The standard substance and the sample to be tested are sequentially added to the well of the enzyme label plate , Thoroughly shake and mix, and then put it at room temperature 2 Hours of incubation process,

Product Specification

protein IL-6
Species Reactivity Rat
Source Rat
Description

Detection principle:

This kit uses double antibody sandwich ELISA Technologies. Specificity anti- Rat IL-6 capture antibody Pre-coated on high affinity enzyme plates. The standard substance and the sample to be tested are sequentially added to the well of the enzyme label plate , Thoroughly shake and mix, and then put it at room temperature 2 Hours of incubation process, present in the sample IL-6 Binding to solid phase antibodies. After thorough washing to remove free and unbound components, biotinylated detection antibody is added for incubation. After washing again, horseradish peroxidase-labeled streptavidin was added (Streptavidin-HRP , SA-HRP) 。 After washing, the signal enhancer was added for incubation, and after washing to remove unbound material, it was added again SA-HRP 。 After washing again, add TMB The chromogenic substrate is incubated at room temperature in the dark from light to develop color. The depth of color reaction and the sample IL-6 The concentration is positively correlated. Add a stop solution to stop the reaction, and use a microplate reader to 450 nm Detection wavelength ( Correction wavelength 570-630 nm) The absorbance values were determined under conditions.

 

Test Type: Double antibody sandwich method

 

Form: Pre-coating 96 Orifice plate

 

Test Sample Type: Cell supernatant, serum, plasma

 

Sample Load: 100 μl

 

Kit Components: Pre-coating 96 Well plate, standard, biotin labeled IL-6 Test antibody, standard dilution, test buffer, signal enhancer, signal enhancer dilution, TMB A chromogenic substrate, a wash liquid, a stop liquid, SA-HRP , a copy of the sealing film and instructions.

 

Sensitivity: 1.80 pg/mL

 

Detection range: 7.81-500 pg/mL

 

Recovery Range: 95-106%

 

Save Method: 2-8℃

 

Standard graph:

 

 

Background:

Interleukin 6(IL-6) It is a cytokine with both pro-inflammatory and anti-inflammatory properties. In humans, it is composed of IL-6 Genetic encoding. During infection and after trauma, especially inflammation caused by burns or other tissue damage, IL-6 Will be by T Cells and macrophages secrete, promoting the immune response. Studies have shown that mice need to resist Streptococcus pneumoniae infection IL-6 Participation, describing IL-6 Plays a role in fighting infections.

IL-6 Inflammatory and autoimmune disease processes can be promoted in many diseases such as diabetes, atherosclerosis, depression, Alzheimer's disease, systemic lupus erythematosus, multiple myeloma, prostate cancer, Behcet's disease and rheumatoid arthritis. later period / Patients with metastatic cancer have higher levels in their blood IL-6 。

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SKU: 7971953670

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4.1 ★★★★★
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Steve
Massapequa, US
★★★★★ 5
Great edger
This is a great lightweight and portable edger. It’s much easier to use than a gasoline powered model. The only limiting factor is the length of the electric cord. It does a great job and make sure your driveways and sidewalk look manicured.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 6, 2026
L
Verified Purchase
Lindybid
Pawtucket, US
★★★★★ 5
Awesome edger
This is our second purchase of this product. We had the first one for over ten years and we ended up breaking it(our fault). Immediately bought same one. This is a reliable machine. It goes right through thick weeds and rock hard dirt to edge a lawn or trench. Like I said our previous one lasted ten years and worked just as well in year ten never replaced the blade once. This one came with a back up blade as well which is a bonus. Highly recommend this. Easy to assemble. Takes five minutes. Durable. Powerful. There is hieght adjustment as well. Well worth the price!
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Reviewed in the United States on July 12, 2024
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Verified Purchase
linda k. bennett
Carnegie, US
★★★★★ 5
edger
given as gift but I liked the weight and ease of handling. Seemed well made and simple to use. I was pleased with size and features.
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Reviewed in the United States on January 1, 2026
M
Verified Purchase
Movie Fan
Carnegie, US
★★★★★ 3
Nice Edger as long as the blade doesn't come loose -- and it will come loose, repeatedly!
Probably could've/should've rated this 2 stars yet it works well when it works. I purchased this edger to edge my drive and sidewalks and not for trenching. The edger arrived very quickly after I ordered it (I am not a Prime member so this was a nice surprise). Assembly was easy and straight-forward. The first use went smoothly and the edger performed flawlessly and provided clean, smooth edging. About 20 minutes into the second use I noticed that the edger was cutting and quickly determined that the blade was spinning freely -- fortunately the hex nut was still attached to the threaded shaft. I reviewed the manual that came with the edger, grabbed the correct sized socket and torque wrench. I removed the hex nut, the first washer, the blade and then the second washer. To install I followed the directions ensuring that the flats of the washer aligned with the flat on the shaft and tightened the hex nut to the specified 140 in-lbs. The edger worked well for about 10 minutes and the blade once again became loose. This time I replaced the original blade (which did not need to be replaced from a wear perspective) with a new B&D blade and new washers. Once again I followed the instructions and this time the edger performed flawlessly until about 5 minutes remaining in my edging when once again the blade came loose. my experience has most certainly been frustrating to say the least. one should not need to carry a socket and torque wrench with them and repeatedly need to tighten the blade. So, when the edger performs as intended it does an excellent job at edging; however I have no confidence that this edger will be able to perform as intended throughout repeated uses and more importantly has shown that it cannot complete my edging without requiring tightening the blade (which requires disassembly down to the second keyed washer). Potential buyers should be prepared for this experience and make your decision accordingly. Note: I tried contacting black and decker via twitter (@blackdeckerhome) and never received a response; I could not find a separate customer service/support twitter account. Trying to contact customer service via the phone these days is an exercise in patience to say the least.
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Reviewed in the United States on November 11, 2022
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Verified Purchase
Robert S.
Bozeman, US
★★★★★ 5
Trimmer results
Was alittle hesitant with this being B&D But I was surprised how great it really is. By far way above my expectations. Works like a charm and does a great job. Chose electric because I really don't have other than a driveway to do. But it really did good around the flower beds. Cord limits you alittle but nothing to cry about. Quiet also but still use ear plugs.
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Reviewed in the United States on May 9, 2026

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