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Description
Human CXC motif ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Breast and Kidney Expressed Chemokine/Chemokine (CXC motif) ligand 14 (BRAK/CXCL14). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Breast and Kidney Expressed Chemokine/Chemokine (CXC motif) ligand 14 (BRAK/CXCL14) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Breast And Kidney Expressed Chemokine;Chemokine ligand 14 (BRAK;CXCL14) ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | BRAK/CXCL14 is a small cytokine belonging to the CXC chemokine family. Mature CXCL14 shares many conserved features of the CXC chemokine subfamily, but also exhibits some differences, such as a shorter N-terminus and five additional amino acids in the region between the third and fourth cysteines. It is expressed at high levels in many normal tissues, and its cellular origin is thought to be fibroblasts. This chemokine is chemotactic for monocytes and can activate these cells in the presence of the inflammatory mediator prostaglandin E2 (PGE2). It is also a potent chemoattractant and activator of dendritic cells, implicated in their homing, and can stimulate the migration of activated natural killer (NK) cells. It also inhibits angiogenesis, likely by blocking chemotaxis of endothelial cells. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.3 ★★★★★
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Product Reviews
★★★★★ 5
Very natural
Color: DRF ESPRESSO BROWN, Color: DRF ESPRESSO BROWN
I just got it depending on how often I wear these usually last a month. It’s good quality! Color and highlights on point.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 10, 2024
★★★★★ 1
Don’t purchase
Color: DRF ESPRESSO BROWN
Tangled right out pack threw in trash
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 19, 2026
★★★★★ 5
Skincare STAPLE
Scent: Collagen, Size: 4 Count (Pack of 1)
I originally bought these before my wedding and used them 2–3 nights a week to help boost hydration and get my skin looking its best. I honestly noticed a difference after just a few uses—my skin looked plumper, more hydrated, and had a healthier glow overall.
What impressed me most is that I didn’t stop using them after the wedding. A lot of beauty products end up being a one-time purchase for a special event, but these became a regular part of my skincare routine. I wake up with softer, smoother, more refreshed-looking skin every time I use one.
If you’re prepping for a wedding, special event, or just need an extra hydration boost, I definitely recommend giving these a try. They’ve become one of the few skincare products I’ve consistently repurchased.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 3, 2026
★★★★★ 5
My Favorite Face Mask for Hydrated, Glowing Skin
Scent: Collagen, Size: 4 Count (Pack of 1)
I’ve tried a lot of face masks over the years, but the BIODANCE Bio-Collagen Real Deep Mask has easily become my favorite. Every time I use it, my skin feels incredibly hydrated, smooth, and refreshed, and the results last much longer than I expected from a single mask treatment.
One of the things I love most about this mask is how deeply moisturizing it is. After wearing it overnight, my skin looks noticeably plumper and feels soft and nourished. Instead of just giving a temporary boost, I’ve found that the healthy glow lasts well beyond the next day. In my experience, my skin maintained a radiant, hydrated appearance for nearly two weeks after use, which is something I haven’t experienced with many other masks.
The hydrogel material feels comfortable on the skin and stays in place well throughout the night. When I remove it in the morning, my skin looks brighter, more refreshed, and noticeably more hydrated.
I appreciate that this mask feels like a true self-care treatment rather than just another skincare step. Whether I’m preparing for a special event or simply giving my skin some extra attention, this is the mask I consistently reach for. The fact that it comes in a multi-pack makes it even better because I always like having one available when my skin needs a boost.
Overall, this is one of the few skincare products that I continue to repurchase because I genuinely notice a difference. If you’re looking for a mask that delivers intense hydration, plumping benefits, and a beautiful long-lasting glow, I highly recommend giving this one a try.I’m
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Reviewed in the United States on May 30, 2026
★★★★★ 5
my all-time favourite skincare treatment
Scent: Collagen, Size: 4 Count (Pack of 1)
literally my favourite mask ever. I first received these as a gift 2 christmases ago and have continued buying them since. they provide almost an in-office feeling treatment. its a pain to wear it all night long, as I dont sleep well with them on but its worth it. your skin feels plump, super hydrated and radiant when you wake up and it last for days. the fit is okay but once you try this product, it won't matter as it is so effective. these are worth splurging on.
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Reviewed in the United States on June 1, 2026
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