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Description
FITC Rat Anti-Mouse CD301b Antibody (S-R737)Product Specification Host Rat Antigen CD301b Synonyms Mgl2 Location Membrane Accession Q8JZN1 Clone Number S R737 Antibody Type Rat mAb Isotype IgG2a,k Application FCM Reactivity Ms Positive Sample C57BL 6 mouse splenocytes Purification Protein G Concentration 0. 2 mg ml Conjugation FITC Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution, 2 to 8 C as supplied
Product Specification
| Host | Rat |
| Antigen | CD301b |
| Synonyms | Mgl2 |
| Location | Membrane |
| Accession | Q8JZN1 |
| Clone Number | S-R737 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
CD301b, also known as MGL2 (macrophage galactose N-acetyl-galactosamine–specific lectin 2), is a type II transmembrane C-type lectin that serves as a phenotypic marker for a distinct subset of skin-migratory dendritic cells and alternatively activated macrophages; it preferentially binds terminal N-acetylgalactosamine residues such as the Tn antigen, is essential for transporting protein antigens to draining lymph nodes, critically instructs the priming and differentiation of naïve CD4⁺ T cells toward a T-helper-2 phenotype, and uses high constitutive expression of PD-L1 to exert a dominant inhibitory control over T-follicular-helper and germinal-centre B-cell responses, thereby shaping the magnitude and quality of antibody-mediated immunity.
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