SKU: 8544273373

Human CCL2/MCP-1 ELISA Kit

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Description

Human CCL2/MCP-1 ELISA KitProduct Specification Description Detection Principle: The double antibody sandwich ELISA method was used in this experiment. The anti human ccl2 mcp 1 capture antibody is coated on the microplate. After incubation, ccl2 mcp 1 in the sample and standard will bind to the antibody fixed on the plate, and the free components will be washed away; Then biotinylated anti human ccl2 mcp 1 detection antibody was added for incubation. After washing to remove

Product Specification

Description

Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. The anti human ccl2/mcp-1 capture antibody is coated on the microplate. After incubation, ccl2/mcp-1 in the sample and standard will bind to the antibody fixed on the plate, and the free components will be washed away; Then biotinylated anti human ccl2/mcp-1 detection antibody was added for incubation. After washing to remove unbound substances, streptavidin HRP was added for incubation. After washing, the chromogenic substrate was added to avoid light for color development. The solution color is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading amount: 100ul

Kit Components:

Pre coated 96 well plates, standards, anti human   One copy of ccl2/mcp-1 detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 18.9  Pg/ml

Detection Range: 15.6 - 1000 pg/ml

Recovery Range: 85-104%

Storage Method: 2-8 ℃

Standard Curve 

Background:

CCL2/MCP-1 is a chemokine that binds to the receptor CCR2 and induces chemoattraction of monocytes. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. In addition, CCL2 promotes Th2 polarization of cd4+ T cells, and CCL2 mediated recruitment of monocytes to inflammatory sites exacerbates atherosclerosisSeverity of sclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytically modified CCL2, including N-terminal pyrrolidone carboxylic acid modified glutamine, downregulated activity but did not bind to the receptor.

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SKU: 8544273373

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Laura Shearn
Bozeman, US
★★★★★ 1
Watch insurance
I purchased a citizen watch from Amazon and decided to get warranty they was pushing 🫸 not knowing I was given a 5 yr warranty from citizen company with purchase! Cannot give opinions on it because I have gotten no information about this program and have not had to use it... but I can contact them anyway....
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Reviewed in the United States on December 1, 2023
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Ryan N
Dallas, US
★★★★★ 5
Solid Timepiece
Color: Grey, Color: Grey
Overall, I’m really happy with this watch. The only small complaint I have is adjusting the bracelet. I picked up a $15 watch repair kit to size it myself, and while it wasn’t difficult, the push-pin links were definitely more of a hassle than I expected—especially compared to my Oyster bracelet with screws. Just a bit time-consuming, nothing major. Once that part was done, though, I’ve loved everything about it. It’s a great everyday watch, surprisingly accurate, and one of those pieces you just don’t get tired of looking at. For what it’s worth, this is coming from someone who also owns a Submariner—not trying to flex, and yes, I fully realize how douchey that sounds. Nothing screams “please validate my life choices” like bragging about an overpriced watch that basically just tells time. But in all seriousness, I only mention it to give some perspective on how much I genuinely enjoy this one.
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Reviewed in the United States on December 10, 2025
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justin himebaugh
Battle Creek, US
★★★★★ 5
Great watch!
Color: Grey
What a great watch for the money, Tissot never disapoints!
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Reviewed in the United States on April 21, 2026
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Chris Antos
Carnegie, US
★★★★★ 5
Works great
Color: Grey
Watch looks great and works great
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Reviewed in the United States on April 26, 2026
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Tony Lorenzo
Grantham, US
★★★★★ 5
Calidad y estilo
Color: Grey
Está muy bonito y queda muy elegante para todas las ocasiones.
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Reviewed in the United States on May 5, 2026

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