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Description
Human MyD88 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Myeloid Differentiation Factor 88 (MyD88). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Myeloid Differentiation Factor 88 (MyD88) in the sample. The absorbance (OD value) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Myeloid Differentiation Factor 88 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Myeloid differentiation factor 88 (MyD88) is a protein encoded by the MYD88 gene. This gene provides instructions for producing a protein involved in signaling within immune cells. The MyD88 protein acts as an adaptor, linking proteins that receive extracellular signals with proteins that relay them within the cell. In innate immunity, it plays a key role in activating immune cells through Toll-like receptors (TLRs), a large group of pattern recognition receptors (PRRs). Generally, these receptors sense patterns shared by various pathogens—pathogen-associated molecular patterns (PAMPs)—or patterns produced/released upon cell damage—damage-associated molecular patterns (DAMPs). Various single nucleotide polymorphisms (SNPs) have been identified in MyD88, some of which are associated with susceptibility to various infectious diseases and autoimmune diseases such as ulcerative colitis. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.3 ★★★★★
Based on 10 reviews
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Product Reviews
★★★★★ 5
Thick & sturdy.
Color: Blue
Great mat! Nice thick, sturdy foam! -- Im using it along my raised garden beds so I can crawl across the area that's covered w wood chips to work. I cover up lumber edges w old staples with one edge too, and can push myself up & slide it along beneath me. Great on my knees and elbows. Eliminates the need to constantly get up & down. Nice thick, sturdy foam! Gets slippery fast if I hit it w the hose!
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Reviewed in the United States on May 24, 2026
★★★★★ 5
Durable and Comfortable Yoga Mat That Lasts
Color: Red
The BalanceFrom Go Yoga All Purpose Exercise Mat has proven to be a long-lasting and reliable piece of equipment. After seven years of use, it still holds up well, showing how durable the anti-tear material really is. The thickness provides great cushioning for yoga, stretching, or floor workouts, making it comfortable to use on hard surfaces without slipping.
The included carrying strap makes it easy to transport to classes or the gym, and the lightweight design is convenient without sacrificing support. Even after years of use, the mat remains easy to clean and hasn’t worn down the way cheaper mats often do. While the surface may show some signs of age, the overall performance has stayed strong, which makes it an excellent value for the price.
Pros: Extremely durable even after years, comfortable cushioning, and convenient with a carry strap included.
Cons: The surface can show wear over time, it may feel bulky compared to thinner mats, and the texture may not suit those who prefer a firmer grip.
A dependable yoga mat that lasts for years while staying comfortable and practical. For anyone looking for a durable, affordable mat, this one delivers excellent long-term value.
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Reviewed in the United States on August 30, 2025
★★★★★ 4
From a Non-Yoga enthusiast
Color: Purple
The first time I bought a Yoga mat, I plucked it out of the bin from either Ross or TJ Maxx. Then, I decided it wasn't for me and I sold the mat. So, I wouldn't know how to pick a good mat if I fell over it. I came here and purchased this one from the reviews. Again, I'm not a die-hard Yoga-er? Yogi? So I'm probably not giving it the "workout" it needs, but here's a few things I noticed. When I unrolled it, it was nice and tight from it's position. I had to lay it out, then lay on it to get it flat and ready to use. My dog was amused by this, but I'm not sure it counts as a position.
It does dent. I've seen where others tear, but so far, mine hasn't yet. But dents? Yes. I can see this as more of a "when" rather than "if." The foam is soft-- it's bound to happen at some point.
No noise noted. No squeaking or shifting, or "fart" noises. You can go about your routine in peace.
I did notice a smell on my hands after. At first, I wasn't sure what it was, but it was latex-esque or plastic like. I didn't notice this during, but I did smell it after.
To me, it's squishy and comfortable. Like I said , I briefly used it as a make-shift cot prior to it's official use, and I thought it was fine. Didn't move, and for what it is, I thought it was pretty thick.
I Love the strap, I love the color, it'll do for now. Until it falls apart, I'm happy with it.
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Reviewed in the United States on September 1, 2019
★★★★★ 5
Squishiest impact absorbing mat I've seen
Color: Red
The size is smallish, and well advertized at 24" X 71", however the quality and padding is there, and at the price point I know it's a winner. It absorbs pressure and rebounds swiftly. It has a matt texture that is a bit grippy, which I prefer. I've paid a lot more for mats that didn't have this shock absorbing performance. I'm excited to own it.
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Reviewed in the United States on May 10, 2026
★★★★★ 5
1/2” yoga mat. Great quality for the money.
Color: Aqua
I bought this yoga mat primarily for the 1/2” padding. I like the color and appearance with the ribbing on one side. I’m an older person and it’s difficult and painful to get down on my knees. This mat is comfortable and so easy to use. Doing my stretches and yoga are so much easier for me.
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Reviewed in the United States on June 1, 2026
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