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Description
Human UCP1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a thermogenin (UCP1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The color intensity is positively correlated with the thermogenin (UCP1) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Thermogenin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Mitochondrial uncoupling protein 1 (UCP1), also known as thermogenic protein and encoded by the UCP1 gene, is a mitochondrial carrier protein found in brown adipose tissue. It is used to generate heat through non-shivering thermogenesis and contributes quantitatively to offsetting heat loss in infants that would otherwise occur due to their high surface area-to-volume ratio. Its mediated heat production in brown fat uncouples the respiratory chain, allowing for rapid substrate oxidation at low ATP production rates. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.7 ★★★★★
Based on 13 reviews
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Product Reviews
★★★★★ 5
Solid, Well‑Built Adapter with Excellent Performance
I bought this adapter for my M1 MacBook Air so I could properly test the speeds on my new 2Gb internet service. During installation, my old cheap USB‑C Ethernet adapter kept capping out at 1Gb, and even the tech suggested the adapter was the bottleneck.
After he left, I ordered this UGREEN model. I’ve had great luck with their products before and it did not disappoint. It has a solid, heavier feel with what seems like a metal casing, and it worked instantly. macOS recognized it right away with no drivers needed. I simply turned off Wi‑Fi, plugged it in, connected the Ethernet cable, and I was online immediately.
My very first speed test hit right at 2Gb, and it’s been consistently fast and stable ever since. I also haven’t noticed any heat issues at all.
Overall, a fantastic upgrade and absolutely worth it if you need true multi‑gig speeds on a Mac.
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Reviewed in the United States on May 8, 2026
★★★★★ 5
Reliable network speed tests and smb speeds
Got this on prime day for about $20 USD. For the price and quality it was definitely worth! Works perfectly and as you would expect. No driver installations or dropped connections so far, just plug and play. Sleek design, gets a little warm (which is expected) but not hot at all.
Tested on compatible internal network speeds which showed to be reliable and fast
Hardware tested on:
- base m4 mac mini connected via usb c ugreen 2.5g adapter
- over cat7 ethernet cable (overkill but cat6/cat5e under 100meters/328ft should be enough)
- 2.5g ethernet switch
- 2.5g port on nas
iperf3 tests:
- iperf3 tests from base m4 mac mini to another 2.5g device on a 2.5g switch
- shows average bitrates of ~2.35 Gbits/sec
smb tests from a usb3 connected to base m4 mac mini:
- only took ~2mins to transfer 31.31 GB for 11 files in varying sizes
- which on average came out to around a transfer rate of 2.087gbps
- which is about 260.88 Megabytes per second
- i'd imagine this would even be slightly faster for files directly on the mac but wanted to test with a real scenario
- probably closer 290-300 Megabytes per second for files stored on the device rather than usb3
have not had a need to test this on mobile yet but iphones with the 10g usbc (and probably just usbc in general) should be fine
i'm sure if you're looking into buying one of these you already know all this but some things to keep in mind. your mileage may vary based on your cable length and runs/environment surrounding your network/cables for any electromagnetic interference. but generally as long as you have 2.5gb compatible ports and cabling and you should be good to go!
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Reviewed in the United States on July 11, 2025
★★★★★ 5
Solid USB-C Upgrade for 2.5GbE - Good Value and Performance - Good for Mac Users
I’ve been using this Ugreen adapter for a 6 months now and it’s become one of my go-to accessories. If you have a device like the M1 MacBook Pro or Mac Mini that’s limited to 1Gb Ethernet, this adapter is a great way to unlock faster speeds, especially if you’re upgrading your home or office network to 2.5GbE. I have everything connected to a 10Gb switch after I upgraded my Synology NAS and needed a way to improve access to it for storage.
Setup was truly plug and play, just connect the Ethernet cable to one end, plug the USB-C into your device, and you’re good to go. I noticed an immediate improvement in transfer speeds when moving large files to my NAS. This is perfect for anyone working with media or large data sets where Wi-Fi just doesn’t cut it. I was initially capped at 200MB/s. It more than doubled when I used the adapter to 200MB/s
At around $20, it’s excellent value when compared to higher-end adapters that cost much more but often deliver the same result. It’s compact, feels well made, and I’ve had zero issues with performance or stability. It does get slightly warm during long transfers, but I haven’t seen any drop in connection quality or speed.
If you’re trying to squeeze more speed out of your Mac Mini, laptop, or even a docked tablet, this is a solid upgrade. I keep mine on the desk and switch it between devices as needed. Highly recommended for anyone moving to a 2.5Gb network or needing faster wired performance.
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Reviewed in the United States on July 27, 2025
★★★★★ 5
MR. SULU… PREPARE FOR WARP SPEED. ENGAGE.
Rocket fast. Sleek, smooth design. Have another adapter from different company, which tends to fail when utilizing more than 1.5 GB. It just decides to downshift the first gear.
This adapter does not.
Speeds are scary.
SpeedTest results are fun, watching the gauge being download teat and swing radically to the right. Pinned.
Speeds coming in at 2,327 mb/s uploads at 2,150 mb/s (depending on time of day.
Great for streaming content from laptop 4K, Dolby Vision Dolby Atmos, no problems… smooth no hiccups
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Reviewed in the United States on May 28, 2026
★★★★★ 5
Great speed, connection sometimes blocked but there is a solution.
I wanted to take advantage of my 3gb fiber connection, and the best way was to connect my Mac M1 via Ethernet. This looked like a good solution. It started pretty good. I could almost max out my 2.5gb port on my switch. Then a strange thing started happening.
At odd, but consistent, intervals, my connection would stop and say it was 'blocked'. If I waited, it would come back to active. If I switched Thunderbolt ports, it would come back instantly. Annoying.
After a couple of days trying different ports, cables, DHCP vs static IP, and WiFi working flawlessly, I figured it was something in the Mac. Many Google searches directed me to the Thunderbolt Bridge. Apparently, this feature is to directly connect two Macs together IP for a high-speed connection. But it has been known to cause my issue.
I removed the Thunderbolt Bridge service (I can add it back later if needed), rebooted, and have been working great for over a day now. Hopefully, this might help someone in the future.
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Reviewed in the United States on May 24, 2026