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Description
Pyrophosphatase, InorganicProduct Specification Species Yeast Synonyms Inorganic pyrophosphatasePPase Concentration 0. 1U l Expression System E. coli Molecular Weight 33. 5 kD Conjugation Unconjugated Tag His Tag Physical Appearance Liquid Storage Buffer 20 mM Tris HCl100 mM NaCl1 mM DTT0. 1 mM EDTA 50% Glycerol (pH 8. 0 @ 25C) Stability & Storage Store at 25 ~ 15 for 2 years Reference [1] Lahti R , Pitk Ranta T , Valve E ,et al. Cloning and characterization of the gene
Product Specification
| Species | Yeast |
| Synonyms | Inorganic pyrophosphatase、PPase |
| Concentration | 0.1U/μl |
| Expression System | E.coli |
| Molecular Weight |
33.5 kD |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM Tris-HCl、100 mM NaCl、1 mM DTT、0.1 mM EDTA 50% Glycerol (pH 8.0 @ 25°C) |
| Stability & Storage |
Store at -25 ~ -15℃ for 2 years |
| Reference |
[1] Lahti R , Pitk Ranta T , Valve E ,et al. Cloning and characterization of the gene encoding inorganic pyrophosphatase of Escherichia coli K-12.[J].Journal of Bacteriology, 1988, 170(12):5901-7 [2] Satoh T , Watanabe M , Nogi S I ,et al. Molecular Cloning, Expression, and Site-Directed Mutagenesis of Inorganic Pyrophosphatase from Thermus thermophilus HB8[J]. Journal of Biochemistry, 1998, 124(1):79-88. |
Background
Pyrophosphatase, Inorganic is a yeast derived inorganic pyrophosphatase (PPase) expressed by Escherichia coli, which is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two molecules of phosphate ions, causing the inorganic pyrophosphate hydrolysis to form phosphates. In molecular biology, it can be used to increase the production of RNA in vitro transcription reactions.
Components
Storage Solution: 0.1U/μl Pyrophosphatase, Inorganic、 20 mM Tris-HCl、100 mM NaCl、1 mM DTT、0.1 mM EDTA 50% Glycerol (pH 8.0 @ 25°C)
Protocol
Use 1-3 units per ml in a high yield in vitro RNA synthesis reaction
Unit Definition
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