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Description
Wilms Tumor Protein (WT1) Recombinant Rabbit mAb (SDT-2864)Product Specification Host Rabbit Antigen WT1 Synonyms Wilms tumor protein; WT33; WT1 Location Cytoplasm, Nucleus Accession P19544 Clone Number SDT 2864 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P, ICC, IF Reactivity Hu, Ms, Rt Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05% BSA, 0. 03% Proclin 300 Stability & Storage 12 months from date of
Product Specification
| Host | Rabbit |
| Antigen | WT1 |
| Synonyms | Wilms tumor protein; WT33; WT1 |
| Location | Cytoplasm, Nucleus |
| Accession | P19544 |
| Clone Number | SDT-2864 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IF |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu |
| ICC | 1:500 | Hu |
| IF | 1:500 | Hu |
Background
Wilms tumor 1 (WT1) gene encodes a zinc finger transcription factor (WT1) that regulates genes involved in proliferation, differentiation and apoptosis, while it plays a central role in the embryogenesis of many tissues including the urogenital system, heart, and central nervous system.Although predominantly expressed in nucleus in normal cells, WT1 shuttles between the nuclear and cytoplasmic compartments. WT1 is detected in functional polysomes; interacts with the splice factor U2AF65; and is associated with nuclear mRNP particles in fetal kidney cells. In WT1‐expressing tumors, WT1 is mostly expressed in the cytoplasm and interacts with the actin cytoskeleton.
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