Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 31 - Sep 5
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human IFNγR ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with interferon-gamma receptor (IFNγR) capture antibodies. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the interferon-gamma receptor (IFNγR) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Interferon-gamma receptor ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | The interferon-γ receptor (IFNGR) protein complex consists of two isoforms, IFNGR1 and IFNGR2. It binds to interferon-γ and is the only member of the type II interferon family. In unstimulated cells, these subunits do not pre-associate with each other but instead associate with the inactive forms of specific Janus family kinases (Jak1 and Jak2) through their intracellular domains. Jak1 and Jak2 associate with IFNGR1 and IFNGR2, respectively. IFN-γ binding to IFNGR1 induces rapid dimerization of the IFNGR1 chains, forming a site recognized by the extracellular domain of IFNGR2. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.3 ★★★★★
Based on 6 reviews
Sort
Product Reviews
★★★★★ 5
“AROMATHERAPY” IN THE SHOWER
LOVE this scent! Strong in shower but mellows once rinsed off (might be too strong for sensitive individuals). It’s an “aromatherapy” experience every time you shower! We like the scent so much we put a squirt or two in our foaming hand soap dispensers (we switched to fragrance free hand soap so we could scent it with this)!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 29, 2026
★★★★★ 5
Just great
Hands down my favorite body wash/scent from cremo. Lathers great, and scent lasts for a long time
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 27, 2026
★★★★★ 5
Men’s body wash
Smells nice and fresh
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 19, 2026
★★★★★ 5
• Create a signature fragrance for a foaming dispenser
Scent: Palo Santo, Size: 16 Fl Oz (Pack of 2), Scent: Palo Santo, Size: 16 Fl Oz (Pack of 2)
One of the features that I enjoy about liquid soap is its ability to quickly refresh skin. This Palo Santo product is certainly one of the most unique offerings on the market; it is warm and pleasant, albeit a short-lasting fragrance as should be expected of any body wash. While most customers will consider using this product exclusively as a body wash, my preference is to enjoy it throughout the day as a hand and face refreshment.
Use a foaming soap dispenser
This product can be used as a daily hand and facial wash. Now, you might be thinking that this would be pretty inconvenient because the product is contained in a talI bottle with a flip-up top that can be both clumsy to use and takes up counter space. To address these problems, transfer the product into a small, foaming pump, counter top dispenser.
Now I know what you’re thinking; how is this possible? This product has a viscosity similar to syrup. It will never pass through a foaming dispenser. And you would be correct in that critique; so don’t use it straight-up from the Cremo bottle. Instead, dilute the product with water. Since I’ve used this method before with other liquid soap products, I know that it will work consistently by following a few simple steps.
The goal is to make a diluted solution of at least 90% warm water and 10% product, in other words a 9-to-1 dilution. Using a smaller ratio with too much product will likely jam the pumping mechanism because the product is too viscous to transit the foaming mechanism.
How would this be done?
Step 1. Select an empty foaming soap dispenser. The brand is not as important as long as you are able to fill it with water and soap product.
Step 2. Fill the dispenser with warm water to reach about three inches from the top of the bottle.
Step 3. Pour in Palo Santo to raise the water level to about one inch above the existing water line.
Step 4. Cover the top of the dispenser with your palm and gently mix the product and water by rocking the bottle, or stir the product until it mixes with the water. I don’t recommend shaking the bottle. Shaking will result in a lot of soapy lather escaping the container before the pump top is reattached.
Note: When filling the container, leave enough air space at the top of the dispenser for the stem and pump mechanism to be returned to the container. Overfilling just means that some product will escape when recapped.
Step 5. After the top is reattached, prime the pump a few times and the foam mixture should smoothly leave the bottle. Photo One shows the water and Palo Santo mixture. As you can see, the mixture is transparent.
The result is an easy to use hand and face foam wash. This pump spray approach means that the Cremo bottle can be stored away until a refill is needed. This process should work for every clear Cremo product.
Create your signature fragrance
The Palo Santo fragrance is unique, but its colorless appearance in a dispenser is not inviting. The product could use some color to make it more appealing and some zhoosh fragrance from another product. To accomplish this, add an extra body wash product that is both colorful and has a complementary fragrance to the original Palo Santo. All it takes is this addition to Step 3:
Step 3-PLUS. Add about a quarter inch of another liquid soap to the container and mix it as in Step 4. The result is now a colorful mixture with a more expressive fragrance.
Three different combinations
In my first trial, Palo Santo was mixed with sage and cedar wood that added an attractive teal color (see photo 2). In the second trial Palo Santo was mixed with mint and rosemary making a blue combo (see photo 3). The third trial was a mixture of coconut and black pepper creating a purple mixture (see photo 4). Each of these combinations produced a complex blend of fragrances that would appeal to family and friends.
Blend Palo Santo to your personal taste
Palo Santo has a reputation for being a “mature” fragrance. Fair enough. But it does need to be—like Old Spice(y) or Hai Karate. Instead, it can be developed into a more chic and modern choice. By adding a dash of extra ingredients, you can design a blended Palo Santo fragrance that is both more complex and appealing. Certainly, with a little experimentation, you can also create a variety of mixtures that enhance Palo Santo and broaden the spectrum of your daily hand and face wash products. Just start with your favorite Cremo product and zhoosh-it-up.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 7, 2023
★★★★★ 5
Screamo for Creamo! Update on Sage and Citrus and Cologne
Scent: Bourbon Oak, Size: 16 Fl Oz (Pack of 2)
So first off the product came exactly as depicted. The smell was good but not strong or offensive. It has a pleasing woodsy smell. The name says it all. The lather is not particularly big but that doesn’t define how clean you get. I think it cleaned me up very well with one caveat. My face is a little oily so I did purchase the Creamo face wash. The body wash really didn’t do the trick for my face which is somewhat oily. The bottle is reminiscent of the past and stylish. It overall is a great product.
I just bought the Sage and Citrus body wash. This is a great all season body wash with a more contemporary smell. It’s lighter than the Bourbon and Oak with, as stated, a citrus smell tempered with the earthy sage. Again it does not clean my oily face but I use the Creamo face wash and it does just fine. The rest of my body feels clean and the scent is light and not overpowering. Between both products I also use the shaving cream.
I enjoy the original scent. I tried the Bourbon scent with the Bourbon and Oak body wash and felt it was a little overpowering. On a good note I combined all this with the Bourbon and Oak cologne. The shaving cream last a long time and should be used sparingly. The value is in the amount you use. It’s far less than a cheaper shaving cream but offers superior protection and excellent moisturizing elements.
The cologne lasts all day and does not require but a single spray under both arms and a single spray on a wrist rubbed against the other. If you need an anti perspirant use one without a scent since this will conflict with the cologne. The value is definitely there as this cologne albeit simple in appearance has very delicate notes reminiscent of my more expensive cologne. I use Creed Vetiver for going out. This cologne has a very similar quality but is, of course, a different bouquet.
Overall the whole line of Creamo products seems to be well thought out and outshines many higher end products such as Clinique or Brickell which I have also used. It comes down to value and quality. That’s where it really wins. It’s made in California where I live!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 23, 2019