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Description
Human IFN-γ OneStep HS ELISA KitProduct Specification Antigen IFN Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Hu Purification Protein A Stability & Storage 12 months from date of receipt reconstitution, 2 to 8'C as supplied. Kit Precision Intra assay: 4. 5% Inter assay: 6. 0% Sample type Serum, Plasma Assay type Sandwich (quantitative) Sensitivity 0. 316pg ml Range 0. 78 pg mL 50 pg mL Recovery Serum: 94% Plasma: 97% Assay time 60 minutes Species
Product Specification
| Antigen | IFN-γ |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 4.5%； Inter-assay: 6.0% |
| Sample type | Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.316pg/ml |
| Range | 0.78 pg/mL – 50 pg/mL |
| Recovery | Serum: 94% Plasma: 97% |
| Assay time | 60 minutes |
| Species reactivity | Human |
| NIBSC convert |
The dose response curve of the International Standard (87/586) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (87/586) units, use the following equation: NIBSC (87/586) approximate value (IU/mL) = 0.02 x OneStep Human IFNγ value (pg/mL).
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Background
Interferon gamma (IFN-γ) is a cytokine critical to both innate and adaptive immunity, and functions as the primary activator of macrophages, in addition to stimulating natural killer cells and neutrophils. A non-IgE-mediated anaphylactic reaction and severe bronchospasm have been reported once after the first injection of interferon gamma. IFN-γ activates cells via a different receptor than IFN-α and IFN-β, which accounts for the different physiological properties of the proteins. Production of IFN-γ is largely restricted to activated CD4+ TH1 T cells, CD8+ T cells, and natural killer cells. One of the most important consequences of IFN-γ secretion is the activation of macrophages. In addition, IFN-γ plays a central role in inflammatory responses by activating endothelial cells, promoting TH1 cell development and cellular immune responses, and up-regulation of major histocompatability complex protein expression on antigen-presenting cells.
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