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Description
Absin 7-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)Product Specification References 1. Mingzhu Yang, et al., Chemical induced chromatin remodeling reprograms mouse ESCs to totipotent like stem cells. Cell Stem Cell 2022 Mar 3 Xin Guan, et al., Nanoparticle enhanced radiotherapy synergizes with PD L1 blockade to limit post surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 Qian Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and
Product Specification
| References | 1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell 2022 Mar 3 Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications Feb 9, 2022 4. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, September 2008. Ed Harlow, David Lane. Technical guidelines for antibodies (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. 7. Money helps the country. Jiao Lei. Application of multi-labeled immunofluorescence staining and Doppler imaging in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Usage | 1. Dilution of fluorescent dye: If the dye is dry powder, use 100ul DMSO to dissolve it to make 100X dye mother liquor; If the dye is liquid, it means that it is 100X mother liquor dissolved in DMSO from the factory; Use the signal amplification reaction solution to dilute at 1: 100 to prepare the dye working solution (ready-to-use); DAPI prepares the working solution using sterile water dilution at 1: 100. |
| Experimental Procedure | https://univ.oss-cn-shanghai.aliyuncs.com/absin-china/images/experiment/20190514165940524.png |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Synonym | Multicolor kit |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single index, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, and then the next primary antibody is replaced for the second round of incubation, and another fluorescein substrate is replaced, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 520, TSA monochromatic fluorescent dye 540, TSA monochromatic fluorescent dye 570, TSA monochromatic fluorescent dye 620, TSA monochromatic fluorescent dye 650, TSA monochromatic fluorescent dye 700, signal amplification reaction solution, pika universal HRP labeled secondary antibody, anti-fluorescence quenching encapsulation tablet, DAPI. |
| General Notes | 1. This kit is only used for immunohistochemistry and is not used for other purposes. 2. This kit is restricted to professional use only. 3. Appropriate protective measures should be taken to avoid contact of the reagent with the skin and eyes. 4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used. 5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process. 6. Incomplete dewaxing will easily affect the dyeing effect. 7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment. 8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management. |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2 ~ 8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell crawling sections, and needs to be matched with abs994 antibody eluate (specific for mIHC). |
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4.2 ★★★★★
Based on 30 reviews
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Product Reviews
★★★★★ 5
One of the best genral-purpose frothers out there, period
For the longest time, I've been looking for what seems like a unicorn -- something about the size of a mini-frother, but stronger than most. Almost a mini-submersion blender, really. And I still haven't found it. But the Maestri House frothers are about the closest thing I have found, which gives me hope that my unicorn might really exist someday.
Unlike cheaper frothers that almost always warn you off from actually washing them, Maestri House makes theirs waterproof. Seriously, you can froth sloppy and get it all up in the whisk end of this, then rinse the whole thing under the faucet if you want to. I really don't recommend that for the speed ring at the top of this one, as a precaution and because it should never be necessary, but you can if you have to.
Despite what I believe is cheaping out on the battery at just 1200mAh, it really can last a while, especially if you use it just once or twice a day. That it recharges using a USB-C connector is definitely a bonus.
The speed ring at the top is great feature, overall. It turns on at a slow speed and lets you crank it all the way up to a speed that I still haven't found a good use for. This provides a lot of flexibility, from gentle mixing to full-on danger frothing.
The included whisks can all be pretty handy. The whisk-whisk is good for things like two or three whole eggs, and the frother-whisk is good for general-purpose frothing. I really haven't figured out what the hook-whisk is for, because this isn't really made for handling any kind of dough, but it might work well for medium-to-thin batters.
Generally speaking, I can easily recommend Maestri House frothers over just about any other handheld frother you're going to find. For completeness, though, there are a few things that I think could be improved upon:
- The battery. Once you're into the $25+ range, I would expect a a high-current 2000mAh battery, for longevity and torque.
- The speed ring is flexible, but depending on your dexterity and grip it can be tricky to operate when dialing in just the right speed, with just one hand. And there's no "Off" button, so you have to wind it down to "Off". For some reason I often end up spinning it the other way and making it go faster instead. Muscle memory should take over eventually, but still. A dedicated On/Off button would be handy.
- For as beefy as this device feels, it doesn't handle thick liquids as well as I expected. The torque for basic mixing and frothing tasks is more than enough, but get this into thicker liquids or even four+ eggs, and it starts to bog down.
- Like most frothers of this type, the balance of the whisks is a variable thing. If a whisk shaft gets even a little bent this thing will shake, at least when started at slow speed. Things usually settle out at higher speeds, but I'd really like to see the shaft of the whisks made thicker and more rigid so they don't bend so easily.
- The frother whisk is good for what it is, and will quickly make great, basic foam. But unless you have supreme technique, it probably won't be good for any kind of latte art. This isn't a big problem in my mind, just something to be aware of.
- The stand is okay at best, and unstable at worst. It's just a bit short for some whisks, so some of them can bottom out on the counter and make it unstable. Bending the holder at the top back just a bit with some good pliers, so the whisks are tilted up and away from the bottom, can help. I'll probably end up designing a 3D printed stand to hold this more security, as well as store the extra whisks.
- I'm really not a fan of the textured finish on this model. It feels good enough, and the color is okay, but there are obvious seams on each side that, in my opinion, take away from the aesthetic. My call would be that whatever finish might be on the body, it should be seamless around the entire cylinder.
I want to be clear, that none of the comments above are intended to warn you off of this if you're looking for a very good, well made frother. I doubt that you can find anything better! I only mention them because nothing is perfect, and to temper the reader's expectations.
This brand, if not this specific model of frother, is almost certainly everything that you are looking for. If there's anything you don't like about this particular model on paper, I urge you to consider literally any other handheld frother they make.
It only falls short compared to my unicorn device, something I don't ever expect to see from anyone else, but really believe that Maestri House could deliver if they think there's a market.
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Reviewed in the United States on March 12, 2026
★★★★★ 4
It is an upgrade from PRODUKT
There's a lot to like with this frother. First off, it gets super-fast. It has good power but struggles a bit through thicker liquids at high speeds. I really got it exclusively for frothing milk and making really smooth chocolate milk. For those things it works well.
I like the stand. I just keep a napkin under it so it will drip dry after rinsing. It is easy to clean because you can just run it on about medium under running water. I've been using it for about 3 weeks now, probably once every other day, and I'm still on the first charge. To me that is excellent battery life.
It spins true, even at high speed. The key is to treat it well and probably make sure to use the stand instead of laying it on the counter. Because of the high RPM, any imbalance or bend at all is really going to be exaggerated.
The only thing I am not crazy about is the control. I don't like that I have to twist it off while in the liquid. I wish there was just a switch along with the adjustment ring.
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Reviewed in the United States on March 28, 2026
★★★★★ 5
I love it!
Color: Black, Size: Rechargeable
This thing works great! It holds a charge for a long time. I love that it has different attachments. I use it mainly for mixing powder into my drinks. It is very powerful. The speed is adjustable too. It feels good to hold too. It lights up when you squeeze the trigger and when it starts losing charge, the light bar gets shorter. I highly recommend it.
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Reviewed in the United States on June 3, 2026
★★★★★ 5
Perfect all-around frother, stand keeps it nice and neat on the counter within easy reach.
Color: Black, Size: Rechargeable
Rechargeable, lightweight, variable speeds and optional whisks all make this the perfect item for your kitchen. You can quickly froth milk for your coffees, whip a small batch of whip cream, and even make mug cakes with the hook attachment. The price is very compatible with the competitors, but the ease of use makes this a top choice.
The variable speeds makes it much better to control than the push button ones. When you let go of the pressure switch it turns off, no need for a dedicated on-off switch. Having the pressure switch on the top makes it extremely easy to use! It fits very comfortably in the hand and with the metal stand, looks quite nice on the counter, ready to use at any time. I highly recommend this InstaWhisk!
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Reviewed in the United States on May 28, 2026
★★★★★ 5
Great product from a great company
Color: Black, Size: Rechargeable
This product works very well. There are several other whips to use also. I absolutely love the cream it makes and that is important so I can enjoy my morning coffee. I used this whisk for quite a while then all of a sudden it stopped working. I wrote to the company to explain. A few days later they sent me a beans new whisk. GREAT item and great company to work with.
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Reviewed in the United States on May 23, 2026