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Description
Murine RNase InhibitorProduct Specification Species Rat Synonyms Ribonuclease inhibitor Expression System E. coli Molecular Weight 50 kDa (Reducing) Purity 95% by SDS PAGE Conjugation Unconjugated Tag No Tag Storage Buffer 20 mM HEPES KOH, 50 mM KCl, 8 mM DTT, 50% Glycerol (pH 7. 6 @ 25) Stability & Storage Store at 25 ~ 15 for 2 years Reference [1] Wanhua, Guo, and et al. High level soluble production of functional ribonuclease inhibitor in Escherichia coli by fusing it
Product Specification
| Species | Rat |
| Synonyms | Ribonuclease inhibitor |
| Expression System | E.coli |
| Molecular Weight | 50 kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Storage Buffer | 20 mM HEPES-KOH, 50 mM KCl, 8 mM DTT, 50% Glycerol (pH 7.6 @ 25℃) |
| Stability & Storage | Store at -25 ~ -15℃ for 2 years |
| Reference | [1] Wanhua, Guo, and et al. High level soluble production of functional ribonuclease inhibitor in Escherichia coli by fusing it to soluble partners[J]. Protein Expression & Purification, 2011. |
Background
Murine RNase inhibitor is expressed in soluble form in E. coli and capable of inhibiting a wide range of RNases (RNase A, B, C). Murine RNase inhibitor was tested by RT-PCR and RT-qPCR, and was compatible with various commercial reverse transcriptase’s and various DNA polymerases. Compared with human RNase inhibitor, murine RNase inhibitor does not contain two cysteines that are very sensitive to oxidation, so it has higher antioxidant activity and is more suitable for high DTT sensitive experiments (such as qPCR).
Components
Storage Solution: 40 U/µL Murine RNase Inhibitor, 20 mM HEPES-KOH, 50 mM KCl, 8 mM DTT, 50% Glycerol (pH 7.6 @ 25℃)
Protocol
Add inhibitor to achieve final concentration of 1 U/μL in your reaction.
During assembly of a reaction, RNase Inhibitor should be added before other components that are a possible source of RNase contamination.
Guidelines
Please avoid repeated freeze-thaw cycles.
Unit Definition
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