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Description
Human TRPC1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with transient receptor potential canonical 1 (TRPC1) capture antibodies. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the transient receptor potential canonical 1 (TRPC1) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Transient receptor potential canonical 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Transient receptor potential channel 1 (TRPC1) is a protein encoded by the TRPC1 gene. TRPC1 is an ion channel located on the plasma membrane of many human and animal cell types. It is a nonspecific cation channel, meaning both sodium and calcium ions can pass through it. TRPC1 is thought to mediate calcium entry in response to depletion of endosomal calcium stores or activation of receptors linked to the phospholipase C system. In HEK293 cells, the unit current-voltage relationship of endogenous TRPC1 channels is nearly linear, with a slope conductance of approximately 17 pS. The extrapolated reversal potential of TRPC1 channels is +30 mV. TRPC1 protein is widely expressed throughout the mammalian brain, with a cortical expression pattern similar to that of TRPC4 and TRPC5. The highest TRPC1 protein densities are found in the lateral septum, an area densely expressed by TRPC4, as well as in the hippocampus and prefrontal cortex, areas densely expressed by TRPC5. TRPC1 was the first transient receptor potential channel identified in mammals. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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★★★★★ 5
Bang for the buck
Color: Polished Rainbow
If you're a male, you can use this for your line ups easily. Less fuckups and more forgiving. This thing is sharp but not sharp enough to hurt you.
I wouldn't recommend using it for shaving your armpit. But it does work with ease if your blade is new.
Down there does the job too fast. But they have to be trimmed before you shave it.
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Reviewed in the United States on May 19, 2026
★★★★★ 5
Perfecr
Color: Matte Black
I really like this tool it quality and has a good weight to it
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Reviewed in the United States on April 27, 2026
★★★★★ 5
Satisfied
Color: Polished Rainbow
Works great
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Reviewed in the United States on March 15, 2026
★★★★★ 5
This book helped me get focused on how to implement ai into my business and make money.
Format: Paperback
This book helped me get focused on how to implement ai into my business and make money.
I bought this book in early spring to get clarity on how to use AI into my day-to-day business. I read through the book quickly because it’s very easy to read. I love the back part of the book because it details step by step how to implement the techniques he uses. I thought wow now I have somebody (who’s already succeeded) to help me figure out how to implement AI. With all the information out there it’s been hard to know what to use and what not to use and what's practical and what's not practical.
From the very first page: Dan does not waste your time. I’ve read countless books over the course of my life, and this easy-to-read book stands out because it inspires and motivates the reader to take action immediately.
The author takes complex concepts, the kind that usually make your eyes glaze over—and breaks them down into clear, step-by-step processes. Instead of just telling you what to do, the author shows you exactly how to do it.
This is a practical book demands action. The author makes it clear: knowledge without action and implementation is worthless. He encourages readers to begin stating,” Don’t wait to start, just start, (do the outreach work) build the processes and practice, practice, practice, that's what this book is all about. Act first and then tweak the process, because nothing will happen if you don’t get started.
Whether you're just starting out or your business has been operational for years, this book will help you. It's not just another good book—it's a resource that helps you move your business in the right (AI Implementation) direction.
I don't often take the time to make Amazon Book recommendations. But this one? It's earned a permanent spot in my backpack, and my desk.
How can this book help you? Real, practical knowledge you can use starting today.
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Reviewed in the United States on September 12, 2025
★★★★★ 5
One word - Awesome, there is nothing like it out there
Format: Kindle
This book is one of the most valuable resources I’ve come across. From start to finish, it’s packed with actionable insights and practical advice you can immediately use. There’s no filler or fluff—every page is focused on delivering value. The author clearly knows the subject matter inside and out, and it shows in the way complex ideas are explained in such an accessible manner. Finding a book that gets straight to the point without sacrificing depth is rare, but this one does exactly that.
What impressed me was the level of detail in every section. The step-by-step explanations, including video explanations, ensure there’s no room for confusion or guesswork. Even topics that might feel overwhelming at first are broken down into manageable pieces, making it easy to follow along and build your understanding as you go. The author left no stone unturned in ensuring the reader has all the tools and knowledge they need to succeed.
I highly recommend reading this book and taking the time to apply what you learn. Its value is unmatched, and the results will speak for themselves. Whether new to the subject or looking to deepen your expertise, this book is a game-changer. It’s not just a good read—it’s a practical guide that delivers real results if you’re willing to put in the effort.
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Reviewed in the United States on January 15, 2025