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Description
Rat ARNTL ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Tissue homogenates and other biological fluids Preparation before testing: 1. Please take out the test kit from the refrigerator 10 minutes in advance and
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Tissue homogenates and other biological fluids Preparation before testing: 1. Please take out the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Aryl hydrocarbon receptor nuclear translocator-like protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Aryl hydrocarbon receptor nuclear translocator-like protein, also known as ARNTL or brain and muscle ARNT-like protein 1 (BMAL1), is a protein encoded by the BMAL1 gene. BMAL1 encodes a transcription factor with a basic helix-loop-helix (bHLH) and two PAS domains. This 62-amino acid protein plays a key role as one of the positive elements in the mammalian autoregulatory transcription-translation negative feedback loop (TTFL), which is responsible for generating molecular circadian rhythms. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissuehomogenatesandotherbiologicalfluids |
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4.8 ★★★★★
Based on 23 reviews
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Product Reviews
★★★★★ 5
Old fashion quality. Love it.
Size: 25mm, Color: Two-Tone/Gold-Tone/White/25mm
I’m so pleased with my Timex watch. I can actually see the numbers and the added feature of the lighted face is great.
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Reviewed in the United States on April 21, 2026
★★★★★ 5
Great watch!
Size: 25mm, Color: Two-Tone/Gold-Tone/White/25mm
Great watch.
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Reviewed in the United States on June 1, 2026
★★★★★ 5
Perfect for Training (Swimming) Purposes.... 5 Star for what it does as a training watch!
Color: Black/Yellow
This is already my 2nd TIMEX which I use for my swimming practices..
This one beats the older model (Timex 51931 Ironman Triathlon) with some interesting changes:
1) The larger digits on this model makes it easier to read the numbers (as some mentioned to do so when running) even when I am swimming, with a quick flick of the wrist at the turn, it's possible to see the minutes and the seconds.... The older model had smaller number, thus not making it possible to do this.
2) The navigation on this model became quite easier. It was already easy on the older model but this one included instructions on screen, such as NEXT, DONE, + or - in order to advance with your practices and training... Plus a chance to change the way you want to see the information of the your laps (Laps in the big digits and splits at the smaller one, or the oposite)
A Real nice add on by TIMEX.
3) A simple but effective wrist band "clip" (the tiny piece of strap that attachs the "extra" wrist band that is left after you put the clock on)
It has a piece of rubber on the inside that locks on at the wrist band and does not make it loose or even letting it go as some other watches do sometimes. This is more effective underwater I think, as it remains the way you set your wrist, as the older model I had did not quite held in place all the time.
4) Plenty of memory (100 laps is really a nice number), maybe more then most will need but, as a swimmer I use to count splits over 100m (or yards) with many laps during one training... It is quite usefull. I go easily on 10 to 15 laps of the memory in one training.
And with the date feature, that reminds when you did these times, it is quite usefull to keep track on the training with a spreadsheet, for instance... (as I do !)
5) A really big START/SPLIT buttom that is great to press when I am swimming laps and have to press myself at each turn at the pool.
Just perfect. Never a miss !
And the feature which was already at most of the models, of the repeat countdown, and the start of a chrono, or a repeat (what I use the most) in order race agains your average rhitym inside a certain time frame that you can set it up ! And of course the flix technology, which is very good to have it... Just flick your wrist (when you set it to ON) and it will show you at night all info that you need.
It is another great watch from TIMEX and it deserves a look, specially for the price. It is worth to pay a bit more if you are like me and uses a lot of the memory for the laps on swimming pool. Maybe for runners it can be quite a lot of memory, but the price difference is worth the change, for some of the options mentioned at this review.
This is a really good watch to have..
Will you definatelly like as we all are at these reviews.
:-) It deserves the 5 stars rating !
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Reviewed in the United States on January 10, 2009
★★★★★ 5
Great sports watch.
Color: Black/Yellow
I have to admit to being a bit of a watch fiend, having one for almost everything I do, but this is one of the most useful, yet cheaper priced items I've come to enjoy. When I work out I like as much information as I can get, so for running and biking I have a watch for that, but for swimming I needed a watch to help me count my laps and log my times. The Timex Ironman 100-lap watch does this quite well. I first got a Timex 50-Lap watch that I was able to find locally, but 50 laps is very limiting so when I found this watch for less money and a bigger face that had the lap/split time and lap number on the screen at all times I knew I had a great product. When you're finished with your laps just save your workout. The watch will then calculate some information like average lap time and best lap. I haven't completely messed with their data, but I think they average your times by throwing out the lap times that are the outliers, which is good for the accidental laps where you hit the wrong button, but it's not as great when you did a few sprint laps and they don't show up as your best time. If your best time isn't on there you can still recall it by going through each lap individually, but I think my sprint laps sometimes aren't listed as my best laps. I haven't messed with this feature much because it isn't important to me, and removing the outliers is good for looking at your actual pace. I have been able to push myself a bit more in the pool by having actual numbers to compare, which is very helpful. The other features on this watch I haven't used much, but for someone who can't afford a GPS watch and can use maps or a track this watch would be great for running or anything with laps or intervals.
It has up to 9 interval timers and once finished it can either stop or repeat the sequence. It can do what's called chrono at the end, where it just switches to the chronograph feature after your timer or interval timers are finished. Overall this is a great watch and for most people who enjoy doing anything with laps look no further.
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Reviewed in the United States on July 5, 2012
★★★★★ 5
Take licking and keeps on ticking
Color: Black/Green
I think it looks great. I love Timex in the summer time when I go swimming. You don't have to worry about it. 🌞🌧🥶
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 17, 2026
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