Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 25 - Aug 30
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Mouse SIRT6 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a Sirtuin 6 (SIRT6) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Sirtuin 6 (SIRT6) in the sample. The absorbance (OD value) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Sirtuin 6 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Sirtuin 6 (SIRT6), also known as sirtuin 6, is a stress-responsive protein deacetylase and mono-ADP-ribosyltransferase encoded by the SIRT6 gene. It appears to play a role in multiple molecular pathways associated with aging, including DNA repair, telomere maintenance, glycolysis, and inflammation. It is a member of the sirtuin family of proteins, which are homologs of the yeast Sir2 protein. It is primarily known as a deacetylase for histones H3 and H4, and through this activity, it alters chromatin density and regulates gene expression. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.5 ★★★★★
Based on 17 reviews
Sort
Product Reviews
★★★★★ 5
Being “Othered” is Real
Format: Kindle
Sky Full of Elephants opens with a haunting and unforgettable image: all the white people walking silently into bodies of water. That beginning alone tells you this is not a book that will play it safe. It is bold, layered, and deeply intentional.
The writing is beautiful and the story forces readers to confront what Black history truly is: American history. The novel doesn’t just imagine a world; it holds up a mirror to the one we’ve lived in and the one we’re still shaping. It explores identity, belonging, grief, and survival in a way that feels both speculative and painfully real.
As someone who grew up attending predominantly white schools, I connected deeply with Sidney’s experiences. Being “othered” (constantly reminded that you are different, that you don’t quite belong) leaves marks that follow you long after childhood ends. Some of the moments Sidney endures felt painfully familiar, and I found myself reflecting on my own younger self while reading.
What struck me most, though, was reading this story as a mother.
I have a biracial daughter, and her experience has been very different. She has never been made to feel like she doesn’t belong. She has never been othered. She has always been rooted in her Black identity, primarily raised by her Black mother, surrounded by family who affirm her. Even after I remarried and joined a Black family, she was embraced fully, never questioned, never treated as “less than,” never made to feel separate.
Reading Sidney’s journey made me profoundly grateful that my daughter’s story has unfolded differently. It also reminded me how much environment, affirmation, and community matter in shaping a child’s sense of self.
Sky Full of Elephants is more than a speculative novel. It is a meditation on race, memory, and belonging. It asks hard questions about America while honoring the fullness and complexity of Black identity.
This book lingers with you. It sparks reflection. It opens conversations.
And for me, it felt both personal and powerful.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 14, 2026
★★★★★ 4
Good Read!
Format: Audiobook
I read this along with my son and his girlfriend in a family “book club”. We had a good discussion about the ending, as we each had differing perspectives, but that was fun! The book was really interesting, and the characters were so well defined and deeply moving. Good read, but the ending left us a little confused.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 16, 2026
★★★★★ 5
Thought provoking
Format: Kindle
Sky Full of Elephants is a work of speculative fiction that begins with the premise that the white population of the United States has been wiped out. Starting a year after “the event” and following Charlie, a man who spent 20 years in prison due to a cowardly lie, and his resentful, biracial 19 year old daughter, who witnessed her only known family drown themselves, it is at its core a quest for identity in its many forms and how trauma can co-opt that search. In rating and reviewing this book, I’m aware that my lens as a Gen-X, cis-het, white woman, will have a differing view from others’ lived experiences. In reading other reviews, I definitely saw points that I didn’t consider, which I hope is the main point of the book. I do think, as a work of speculative fiction, that it does require the suspension of disbelief from the get go. It is a philosophical “what if” that Mr. Campbell invites the reader to consider.
Intrigued by the premise, I was drawn into the story due to Mr. Campbell’s lyrical writing style. The narrative had a rhythmical flow to it that supported the world building and characterizations. I found Charlie to be a very sympathetic character, rebuilding a life shattered by lies in a new world and confronted with the daughter he never had a chance to know. Sydney, Charlie’s daughter, was more of a struggle to empathize with. While her feelings were justified and understandable, her growth throughout the novel was erratic. As the story has an ambiguous ending, perhaps her character will continue to improve. For supporting cast, the grumpy pilot Sailor and his nonbinary child, Zu, offer a counterpoint to Charlie and Sidney’s emerging relationship. The king and queen of Alabama and the thriving town of Mobile were well fleshed out. The Walkers and Sidney’s Aunt Agatha in Orange Beach represented those who were lost in their own way, either due to clinging to their former proximity to whiteness or to the religious biases they were raised with. I found the Walkers to be the most tragic of all.
The questions of identity throughout the story are what kept be invested throughout. Are we defined by the color of our skin, our behaviors, the groups we belong to, the choices we make? Are others more valuable or worthy who don’t suffer the same things we do? Does there have to be those that are “lesser” to make us feel whole? As a trauma survivor with C-PTSD, I struggle with my own issues of identity and worthiness, and as a former Special Education teacher, I’ve been witness to that struggle in others. I have never understood or accepted the idea of White Supremacy or Christian Supremacy or any of the myriad ways that humanity continues to other each of us. In reality, there is no “us” or “them” only”we”. Charlie questions who he is as a Black man in the US, a convict, a teacher, a father, and ultimately a fixer and healer. Sidney grapples with her biracial otherness, her wealthy upbringing and sheltered life, the trauma of abandonment, and the lies that her life was built on.
The ethical question of the machine at the epicenter of the event adds another layer to the story. While the effects of the first usage were unintended, once they were known is it right to continue to fix it and use it again? Can healing a part of collectiveness that harms or destroys another part ultimately be worth the cost? The world and its people are broken and desperately need healing. But just like the question of eugenics, what of value is lost when specific traits are universally stripped away? And who gets the to decide what is of value anyway? The ambiguity of the ending doesn’t answer the question entirely of what happens when the machine is repaired, but Charlie’s ability to fix things leads me hopeful. Personally, I cared enough about these characters to be interested in a sequel.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 24, 2026
★★★★★ 5
A book that makes you think!
Format: Paperback
This was a super good read, very imaginative. It dealt with identity, belonging, insecurities, family matters. The way it was written was unlike any book I’ve read this year.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 4, 2026
★★★★★ 3
The Possibilities
Format: Kindle
Despite its potential, this book ultimately failed to resonate with me. I found myself repeatedly compelled to put it down, as the focus on the empowerment aspect was overshadowed by the narrative’s preoccupation with re-triggering ourselves through the perpetuation of a harmful lie. This lie, which has tragically cost many Black men their lives and livelihoods, diverted our attention from the more profound themes of rebuilding culture, redefining ourselves, and creating a new world. Instead of exploring the possibility of a beautiful utopia, we were subjected to a process of de-centering ourselves and centering them, their likeness, and the relentless pursuit of proving our worth. While there were indeed wise words that moved me, I was left questioning the purpose of dedicating so much time to those who did not share our sentiments. Who are these individuals who required our convincing, and who are we who felt compelled to do so? I found Sydney, her family, and the inhabitants of Orange Beach to be unlikable characters. I fear that the plot was compromised when the focus shifted to inclusion.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 5, 2026