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Description
Mouse SOCS-1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Preparation of standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 4000pg/mL). Then dilute to the following concentrations: 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 4000pg/mL standard working solution into the first EP tube and mix thoroughly to make a 2000pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube is used as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotinylated detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against suppressors of cytokine signaling 1 (SOCS-1). After incubation and washing, the assay is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of suppressors of cytokine signaling 1 (SOCS-1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse suppressors of cytokine signaling 1ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Suppressor of cytokine signaling 1 (SOCS-1) is a protein encoded by the SOCS1 gene. Homologs of SOCS1 have been identified in several mammalian species for which complete genomic data are available. This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as the suppressor of cytokine signaling (SOCS) family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. Expression of this gene is induced by a group of cytokines, including IL-2, IL-3, erythropoietin (EPO), GM-CSF, and interferon gamma (IFN-γ). The protein encoded by this gene acts downstream of cytokine receptors and participates in a negative feedback loop to attenuate cytokine signaling. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 62.5-4000pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.6 ★★★★★
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Product Reviews
★★★★★ 3
Good finish; but slow dry down, runny, stains clothes.
Size: 1.1 Fl Oz (Pack of 1)
I use this in the lightest shade. It's runny, and it takes about 15 minutes to dry down. It also transfers and sometimes stains my clothes if I'm not careful. The stains do come out, but meanwhile I'm walking around with a dirty shirt. So WHY have I purchased it repeatedly? Once it does dry down, the finish is beautiful. It's radiant, but not overly glowy. On my normal (not oily or dry) skin, I don't need a moisturizer with it. Most importantly, the color is the perfect shade for me. I get compliments on how good I look every time I wear it. I wish I could find a skin tint or balm in this color, so I could use it over a clear sunscreen. Until I find one, I'll have to keep fighting the Purescreen Tinted. Anyone have any suggestions?
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Reviewed in the United States on June 5, 2026
★★★★★ 5
Nice tinted anti-aging sunscreen
Size: 1.1 Fl Oz (Pack of 1)
I really, really like this sunscreen. I got the light color, and it fits close to my skin color.
It has 21.6% zinc oxide which protects against UVA rays, and it has 30 spf for UVB rays. It has a dewy, shiny look and oily texture. Zinc oxide is a photo stable mineral UVA ray blocker, so this sunscreen doesn't degrade over time when you're in the sun. And this product has one of the highest concentrations on the market of zinc oxide (21.6%).
I'll probably keep buying more in the future when I run out of this bottle. I bought a matte primer to apply first so that it mattifies this sunscreen when I put it on. Great product. I recommend.
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Reviewed in the United States on August 29, 2025
★★★★★ 5
One of the best affordable tinted minerals
Size: 1.1 Fl Oz (Pack of 1)
So I have tried many , many mineral sunscreens and chemical sunscreens. I prefer mineral for my face to prevent / treat hyperpigmentation. My favorite holy Grail is skinceuticals tinted mineral , but it's very expensive. I'm always on the hunt for a more affordable option. When I first got this I almost returned because it's not a tinted mineral in the sense that it evens out skin tone or hides anything (it's a peachy color to cover the white cast ) However , I like the ease of application and no white cast. It works great under my makeup and looks flawless. I've even done just concealer and bronzer and it still looks good. It's very hydrating / shiny though so if you don't have dry skin like me , you will not like it. If you skipped moisturizer it could be perfect. This is even a good one to wear to the beach and reapply without worrying you look like Casper. Lastly my bottle has NOT leaked like some other reviews have stated. I close the cap tight and when I apply it I hold it upright so it won't leak out ( it's a more liquid fluid to help it apply easily). I recommend !
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Reviewed in the United States on July 11, 2024
★★★★★ 5
Trusted product to deliver results
Size: 1.1 Fl Oz (Pack of 1)
Great product. Good percentage of sunscreen in this moisturized facial tint. Purchased in light. Goes on effortlessly
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Reviewed in the United States on May 27, 2026
★★★★★ 4
Very good
Size: 1.1 Fl Oz (Pack of 1)
Pros:
-very moisturizing for dry skin. Leaves skin glowy AF. Avoid if you’re oily.
-The pigment is great. Doesn’t look orange
-Doesn’t burn eyes at all.
-Rubs in easy
Cons:
-Is kind of greasy. Once it dries, it’s okay but it takes a little while to dry. It’s really not that bad though, especially if you have dry skin and want a super glowy look.
-Smells kind of strong. It doesn’t have that typical coconut, fragranced smell like traditional sunscreens do. It says it’s fragrance free but it does have this distinct sunscreen smell. I don’t mind it, but it is very noticeable.
-The tint doesn’t provide any coverage for blemishes or anything like that. I think it sort of helps balance out skin tone and a bit of texture, but overall it doesn’t provide any coverage.
-It’s on the smaller side. For 18 bucks, It’d be nice to get a larger tube. But I think it’s worth it because the formula is very nice for a drugstore brand.
Overall, very good sunscreen especially for drugstore.
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Reviewed in the United States on January 15, 2025