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Description
Mouse IgG (Total) OneStep ELISA KitProduct Specification Antigen IgG (Total) Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Ms Purification Protein A Stability & Storage 2 to 8 C as supplied. Kit Precision Intra assay: 4. 4% Inter assay: 6. 1% Sample type Cell culture supernatant, Serum, Plasma, Cell Tissue Lysate Assay type Sandwich (quantitative) Sensitivity 16 pg ml Range 78 pg ml 5000 pg ml Recovery Cell culture supernatant 104% Serum 107% Plasma 103% Assay
Product Specification
| Antigen | IgG (Total) |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision |
Intra-assay: 4.4%； Inter-assay: 6.1% |
| Sample type | Cell culture supernatant, Serum, Plasma, Cell/Tissue Lysate |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 16 pg/ml |
| Range | 78 pg/ml - 5000 pg/ml |
| Recovery | Cell culture supernatant 104% Serum 107% Plasma 103% |
| Assay time | 60 minutes |
| Species reactivity | Mouse |
| CROSS REACTIVITY | / |
| INTERFERENCE | / |
| NIBSC convert | / |
Background
Immunoglobulin G (IgG) is a type of antibody. IgG is the most common type of antibody found in blood circulation. IgG molecules are created and released by plasma B cells. In mice, the IgG class is further divided into four subclasses: IgG1, IgG2a/ IgG2c (strain specific), IgG2b, and IgG3. The mean serum Ig levels found for 6-month-old B6 mice were 0.22 mg/ml for IgM, 0.28 mg/ml for IgG1, 1.22 mg/ml for IgG2b, 0.18 mg/ml for IgG3, 0.075 mg/ml for IgA and about 0.7 mg/ml for IgG2a. This kit is for the quantitative measurement of mouse total IgG in mouse serum, plasma and supernatant from cell cultures.
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