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Description
Nucleoprotein His Tag, HRSV(Strain A2)Product Specification Species HRSV Synonyms Nucleoprotein, Protein N, Nucleocapsid protein Accession P03418 Amino Acid Sequence
Product Specification
| Species | HRSV |
| Synonyms | Nucleoprotein, Protein N, Nucleocapsid protein |
| Accession | P03418 |
| Amino Acid Sequence | MALSKVKLNDTLNKDQLLSSSKYTIQRSTGDSIDTPNYDVQKHINKLCGMLLITEDANHKFTGLIGMLYAMSRLGREDTIKILRDAGYHVKANGVDVTTHRQDINGKEMKFEVLTLASLTTEIQINIEIESRKSYKKMLKEMGEVAPEYRHDSPDCGMIILCIAALVITKLAAGDRSGLTAVIRRANNVLKNEMKRYKGLLPKDIANSFYEVFEKHPHFIDVFVHFGIAQSSTRGGSRVEGIFAGLFMNAYGAGQVMLRWGVLAKSVKNIMLGHASVQAEMEQVVEVYEYAQKLGGEAGFYHILNNPKASLLSLTQFPHFSSVVLGNAAGLGIMGEYRGTPRNQDLYDAAKAYAEQLKENGVINYSVLDLTAEELEAIKHQLNPKDNDVELHHHHHHHH |
| Expression System | E.coli |
| Molecular Weight | 45kDa |
| Purity | >95% by SDS-PAGE&HPLC |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 150mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | 12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles. |
| Reference |
1、Oliveira A P. et al. (2013) Human respiratory syncytial virus N, P and M protein interactions in HEK-293T cells. Virus Res. 177(1): 108-112. 2、Céspedes P F. et al. (2014) Surface expression of the hRSV nucleoprotein impairs immunological synapse formation with T cells. Proc Natl Acad Sci USA. 111(31): 3214-3223. |
Background
Human Respiratory Syncytial Virus (HRSV) is a negative-sense single-stranded RNA virus that belongs to the Paramyxoviridae family and the Pneumovirinae subfamily, and considered the most important pathogen causing respiratory disease in infants and neonates worldwide. The nucleoprotein of HRSV, a canonical cytosolic protein, is highly conserved among pneumoviruses and interacts with viral RNA, generating a helicoidal nucleocapsid which is responsible for genome and anti-genome resistance to RNAse. In addition, the nucleoprotein could be responsible for inhibiting T-cell activation during infection by HRSV, and expressed on the surface of epithelial and dendritic cells, preventing immunological synapse assembly by naïve CD4+ T cells. Therefore, this protein is defined as a major virulence factor that contributes to impairing acquired immunity and enhances susceptibility to reinfection by HRSV.
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4.7 ★★★★★
Based on 21 reviews
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Product Reviews
★★★★★ 3
real good
Format: Kindle
This was a solid read for the ABO universe. The characters are great. The smut scenes were well written. And the town is so cute I want to go there. It didn’t have much character development it just felt like I was getting to the characters as they were getting to know each other.
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Reviewed in the United States on April 27, 2025
★★★★★ 5
So sweet
Format: Kindle
It’s so unexpectedly sweet that it sneaks up on you. Each character is so unique and charming. I love the openness and honesty among their pack. It really blows me away how wonderful this omegaverse trope plays out.
I received a free copy of this book and am voluntarily leaving a review.
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Reviewed in the United States on March 21, 2026
★★★★★ 2
Needs editing
Format: Kindle
Cute storyline and promising characters, but the lack of editing is painful to read. I would love to read an edited version.
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Reviewed in the United States on April 10, 2025
★★★★★ 5
A good read
Format: Kindle
A good read, just fluffy cuteness, no antagonism. I like all the characters. It could have used another round of editing however, glanfds being one error that cracked me up, and my personal pet peeve was that the author kept using the word fill instead of feel, which I promise you are not interchangeable haha, but it's definitely better than the majority of books I read on here mistake-wise.
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Reviewed in the United States on November 28, 2024
★★★★★ 4
amazing
Format: Kindle
Knot the Bride was a fantastic read! The characters were all amazing and well-developed. It was easy to like them all. Sophia, Luca, Nick, and Gavin were all perfect for each other. It was such a charming story that had me hooked the entire time. I did wish there were POVs from Luca, Nick, and Gavin but it was still an amazing book without it.
I am excited to read the next book in the Willowside Omegaverse series! This is definitely a must-read for fans of omegaverse romance!.
I received a free copy of this book and am voluntarily leaving a review.
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Reviewed in the United States on April 25, 2025