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Description
Human ERO1α ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an ERO1-like protein alpha (ERO1α) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of ERO1-like protein alpha (ERO1α) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human ERO1-like protein alpha ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | ERO1-like protein alpha is encoded by the ERO1L gene. Diseases associated with ERO1A include cholera. Pathways associated with ERO1A include translational control and peptide hormone metabolism. An important homologue of this gene is ERO1B. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.7 ★★★★★
Based on 26 reviews
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Product Reviews
★★★★★ 5
Great and affordable cleaner
Color: White
Just got my B&D steam cleaner. I really like it. It is quiet and makes quick work of cleaning the floors instead of scrubbing with a mop. It works great on my floors especially the wood and flat surfaces. I tried it on the ceramic floor and it definitely helped! The problem now is the grout, the cleaner can’t really reach that. This cleaner is light and easy to use. Yes, the cord is short but the extension cord helped a lot. If you order one, make sure to order extra pads because you go through them quick and it only comes with one.
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Reviewed in the United States on April 12, 2026
★★★★★ 5
Efficient, Lightweight, and Chemical-Free Cleaning
Color: White
I've been using the BLACK+DECKER Steam Mop HSM13E1 for several months now, and it has significantly improved my floor cleaning routine. This steam mop is incredibly lightweight, making it easy to maneuver around furniture and tight spaces. The 13.7-ounce water tank heats up in just 20 seconds, allowing for quick and efficient cleaning sessions without the need for harsh chemicals.
One of the standout features is its ability to kill up to 99.9% of germs and remove tough dirt using only water, which is perfect for households with children and pets. The swivel head design allows for easy navigation, and the mop pad is machine washable, making maintenance a breeze.
Whether you're dealing with tile, hardwood, or laminate floors, the BLACK+DECKER Steam Mop HSM13E1 delivers consistent performance and value. It's an excellent choice for anyone looking to maintain a clean and hygienic home environment without the use of chemical cleaners.
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Reviewed in the United States on April 25, 2025
★★★★★ 4
Making Cleaning a Breeze with the BLACK+DECKER Steam Mop!
Color: White
I recently purchased the BLACK+DECKER Steam Mop, and I have to say, it has become my new best friend when it comes to cleaning. This steam mop has revolutionized my floor cleaning routine, and I can't help but share my positive experience with it.
First off, let's talk about convenience. The BLACK+DECKER Steam Mop is lightweight and easy to maneuver, allowing me to effortlessly glide across my floors. I no longer have to lug around heavy buckets of water or deal with messy mop heads. With this steam mop, all I need is water, and I'm good to go. It's a true time-saver and makes the whole cleaning process much more enjoyable.
The steam power of this mop is impressive. It generates a powerful burst of steam that effectively sanitizes and cleans my floors. I love how it effortlessly cuts through stubborn dirt, grime, and even sticky messes. It's a game-changer, especially for high-traffic areas or homes with kids and pets.
One of the standout features of the BLACK+DECKER Steam Mop is the adjustable steam setting. Whether I'm cleaning delicate hardwood floors or tackling tough tile and grout, I can easily adjust the steam intensity to suit the task at hand. This versatility ensures that I can achieve optimal cleaning results without damaging my floors.
I appreciate the quick heat-up time of this steam mop. Within seconds, it's ready to produce steam, allowing me to dive right into my cleaning routine. Additionally, the continuous steam flow ensures consistent cleaning without any interruptions. No more waiting around for the mop to catch up!
The swivel steering feature is fantastic. It allows me to effortlessly navigate around furniture, corners, and tight spaces. I can easily reach under tables, around toilets, and behind appliances with ease. It's incredibly maneuverable, making my cleaning sessions much more efficient.
Cleaning and maintaining the BLACK+DECKER Steam Mop is a breeze as well. The microfiber pad is easy to attach and detach, and it's machine washable, which means I can keep it fresh and ready for the next cleaning session. The mop's water tank is also easy to fill, and I appreciate the clear indicator that lets me know when it's time for a refill.
In conclusion, the BLACK+DECKER Steam Mop has exceeded my expectations. It's a reliable, efficient, and user-friendly cleaning companion that has made my floors shine like never before. If you're in search of a steam mop that simplifies your cleaning routine and delivers excellent results, look no further. This steam mop is a true gem!
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Reviewed in the United States on May 30, 2023
★★★★★ 5
Best Steam Mop for your $$
Color: White
I bought the Black and Decker steam mop 9 months ago. Initially, I agreed with most of the concerns until I learned the problem usually was me and not the mop. For example, the electric cord to short. Yes, but then I learned to coordinate my mopping with the locations of my outlets. Now cord length is perfect! Even wrapping the electric cord after use turned out OK when I learned to secure the plug end at the top of the handle first, then looping the remaining cord around the hook a couple of times. No more problem. This mop doesn't last. My first mop broke (steamer) with two weeks of use. B & D replaced it. Now, I know to refill the tank before it goes dry. Conclusion: I think the B & D mop is the best value on the market for steam cleaning household floors!
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Reviewed in the United States on October 20, 2025
★★★★★ 1
DO NOT BUY - NOT USER FRIENDLY
Color: White
This mop has the worst design and cheaply made. I do not recommend buying it.
Cons: the cord is too short; there is no place on the mop to wind up the cord when you put it away. The cord just flops all over the floor. HOW CHEAP IS IT NOT TO PUT HOOKS ON IT SO THE CORD CAN BE WOUND UP? The mop does not stand on its own. It goes straight to the floor no matter what position you put it in it will not hold an upright position. It lays in the floor. The water compartment does not come off the mop. You have to pick the whole mop up and hold it under the faucet to fill. Or lay it on the floor and get a glass of water to fill. Makes a mess. One more big gripe - THERE ARE NO SETTINGS OTHER THAN ON /OFF AND THOSE BUTTONS ARE WERID. There is no high, med, OR low setting. Lastly the mop pad in the photos was not the one it came with. It had one very thin like disposable one. PROS- NONE. I RETURNED IT .
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Reviewed in the United States on April 20, 2026