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Description
Rat Na-K-ATPase ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Na-K-ATPase (Na-K-ATPase) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Na-K-ATPase (Na-K-ATPase) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Na-K-ATPase ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Na-K-ATPase (Na-K-ATPase) is an enzyme present in the cell membranes of all animal cells (an electrically charged transmembrane ATPase). It plays several roles in cellular physiology. The Na⁺/K⁺-ATPase enzyme is active (i.e., it uses energy from ATP). For every ATP molecule used by the pump, three sodium ions are exported and two potassium ions are imported, resulting in a net output of positive charge per pump cycle. This enzyme belongs to the P-type ATPase family. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
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4.4 ★★★★★
Based on 8 reviews
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Product Reviews
★★★★★ 5
It’s a YES from me!
Color: B-brown
I absolutely love this purse. And I’m so heartbroken because something got spilled inside of it and it affects the functionality of the person on the inside and it makes me so sad but I still use it anyway because there’s one side I can use completely and half a side I can use on the other side lol. It is very good quality. The storage is A+. It is definitely a good value for the price. I was skeptical at first as we all are ordering a purse off Amazon, that is not real leather, but it’s supposed to be good quality. It didn’t really have much of a smell. I actually don’t use the strap provided since I seen that the straps could be interchangeable, I used my strap off my previous purse instead, and now I love it love it! 😍 I get compliments on it all the time!
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Reviewed in the United States on December 28, 2025
★★★★★ 5
Perfect size!
I love a good large hobo bag! This one has a great slouch to it and you can fit a ton in it! I've had everything from my iPad and a makeup bag plus yoga clothes and still had room for a water bottle too! The fabric is gorgeous and although I'm not always keen on logo-prints, I like that the bag's print is so understated and discreet.
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Reviewed in the United States on May 22, 2026
★★★★★ 5
Large, non zipping, sturdy, stylish
large cream MK purse
I’m giving my thoughts on this purse that I got recently and here is what I liked.
Size-
This is a large purse. It has one large main compartment that is secured with a gold, snapping clasp. It doesn’t come apart unless you push both sides of this clasp.
1 pocket inside for my phone or whatever I need quick access to.
2 vertical outside pockets with working zipper. The pockets run the height of the purse and are narrow. I can get a skinny water bottle in there.
Not Adjustable
This comes with a short shoulder strap. The strap is not removable. There are two D-rings (H-ring) on each side that hold the strap so if I want I can add a long strap. Really though, this is not the kind of purse that would look good with a long strap. There is plenty of room to put the purse on your shoulder and it feels very secure.
Look and feel
It looks and feels like leather to me but it says PU which is not leather. I’m fine with that. It is firm in a sturdy strong way, having structure and can stand up on a table. The material is the kind you can wipe with a damp cloth. This purse is not going to get stained unless it’s something like ink that stains practically everything. Mine has monogram NK but if you don’t look real close, I think it looks a lot like a designer purse with the monogram m and k.
Function
I tend to change purses a lot so I keep things in smaller bags. For example, I have small bags for makeup, medical etc and they fit neatly in here. There is not a designated place for cards and cash so you’d need a wallet or you could put it in the inside pocket. I use a medium sized wallet. My iPhone 14 with case fits in the inside pocket and hasn’t fallen out yet. The 2 outside zipper pockets are large enough to hold my phone and my wallet, one on each side.
I can’t think of anything I’d add or change right now. This is a very nice bag to carry everyday. The holding capacity is similar to the neverfull.
I’m happy I got this and can recommend it to you. It’s nice looking, makes good use of space, and for me, was a good deal.
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Reviewed in the United States on March 15, 2026
★★★★★ 1
👎🏻
Color: Beige
0 stars
Poor quality
Arrived torn
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 25, 2026
★★★★★ 2
Quality is not there, I am very disappointed!
Color: Pink, Color: Pink
I am one of those very rare ladies that will wait until her previous purse is on its last leg before I buy a new one. That is when I decided I needed this one. Once I opened the package I noticed a very distinct odor, it was very obviously the purse and it was not a "new" smell it was very off-putting. The purse does not seem very durable as I noticed loose threads throughout the lining of the purse. I was also a little disappointed that this purse is so large but it only had one very large square pocket on the inside. The only reason I gave this more than one star is because of the two very nice pockets on the purse front. I appreciate what they were trying to do, I do not think they were quite up to par on the quality, I would not recommend this item.
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Reviewed in the United States on March 11, 2026