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Description
Human Gliomatosis Cerebri Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4  & Nbsp& Nbsp& Nbsp& Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes (2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A . |
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| Description | Composition:
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| Storage Temp. | 4 ℃, 3months; -20 ℃, 1 year, see the label for details |
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4.9 ★★★★★
Based on 10 reviews
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Product Reviews
★★★★★ 5
True To Size
These shorts fit true to size and are so comfortable. They have a drawstring waist, so you can adjust them for the best fit and wide legs. They are 55% linen and 45% cotton so they keep you feeling cool. The classic colors go with nearly any other colors or designs, making them stretch your wardrobe potential and saving you money. They were extremely wrinkled upon arrival, so I used my fabric steamer, and within 5 min they looked perfect. I love these shorts and wear them all the time!
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Reviewed in the United States on May 27, 2025
★★★★★ 4
Cute 2-pack pair of shorts
Color: Black, White, Size: Medium, Color: Black, White, Size: Medium
Cute shorts that have pockets and fit true to size. I love that they come in a pack of two as well. The black ones are my favorite and I feel like the fabric is a little softer on the black pair. The white ones are a little thinner than I would like. I was wearing an olive green colored underwear underneath them in the pictures in my review and though you can’t see them much, I do feel like they were slightly visible. The white pair also had a lot of loose strings on them which made me think the quality might not be as good as with the black pair. They washed easily and I hung them up to dry them because they smelled kind of funny when they arrived.
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Reviewed in the United States on June 7, 2025
★★★★★ 4
Good value for 2 shorts
These come vacuum sealed and have crazy wrinkles... so be prepared to do some ironing. I ordered the Mediums as I am a 6-8. The black pair feels a bit smaller than the beige pair... with both feeling tighter than I like for comfort. The look nice on, but feel like it constricts my belly a bit too much. Overall a nice basic set of shorts, perhaps size up for a more comfortable fit.
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Reviewed in the United States on June 15, 2025
★★★★★ 5
Super soft and comfortable!
I haven't bought new underwear in forever. I used to wear thongs, but now find them incredibly uncomfortable. The last few times I've tried to buy new underwear, the sizing was either way off (I'm a medium, but gave up after having to return them for a large and then extra large) or the quality was horrible (two brands stretch waaaay out and they can fall down while wearing them (I have larger ass-ets, so they shouldn't start to slide down during use). I tried these after buying some bamboo pyjamas and I love them! They are incredibly soft, comfortable, and stretchy. I can use them all day, and they work well, whether I'm lounging on the couch or wearing them under my spandex shorts at the gym, they don't ride up or slide down. They are both comfortable and look sexy, while providing support in all the right places. I haven't had them too long, so I can't speak on the durability, but they appear to be of good quality and I plan to order more, this time without the band.
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Reviewed in the United States on November 13, 2025
★★★★★ 5
Well made, light fabric. Waist doesn't roll.
These bamboo underwear really do keep you cool.
They fit true to size, and wear easily. the wide elastic waist band doesn't roll on me.
They are comfortable to wear, washed and dried easily.
The material has some stretch to it, but not a lot.
Well made, no stray strings. Perfect fit for me.
Just what I was looking for.
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Reviewed in the United States on May 17, 2026
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