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Description
Alexa Fluor® 488 Mouse Anti-Human TCR α/β Antibody (S-R686)Product Specification Host Mouse Antigen TCR Synonyms TCR; TCR Location Cell membrane Accession P01848P01850 Clone Number S R686 Antibody Type Mouse mAb Isotype IgM,k Application FCM Reactivity Hu Positive Sample Human PBMC Concentration 0. 2 mg ml Conjugation Alexa Fluor 488 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution, 2 to 8 C as supplied Dilution
Product Specification
| Host | Mouse |
| Antigen | TCR α/β |
| Synonyms | α/β TCR; TCR αβ |
| Location | Cell membrane |
| Accession | P01848、P01850 |
| Clone Number | S-R686 |
| Antibody Type | Mouse mAb |
| Isotype | IgM,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
TCR α/β protein is the defining heterodimer of the αβ T-cell lineage, a trans-membrane clonotypic receptor whose extracellular N-terminal variable (V) and joining (J) domains of the α-chain pair with the V, diversity (D) and J domains of the β-chain to create a single antigen-binding site that is stabilized by the constant (C) domains and an inter-chain disulfide, while the short cytoplasmic tails lack intrinsic signaling capacity and therefore associate non-covalently with the CD3 γ, δ, ε and ζ dimers to form the intact T-cell receptor complex that triggers phosphorylation cascades upon recognition of a specific peptide bound to a self-MHC molecule, thereby initiating the adaptive immune response.
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