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Description
Mouse AIMP1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Preparation of standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 2000pg/mL). Then dilute to the following concentrations: 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 2000pg/mL standard working solution into the first EP tube and mix thoroughly to make a 1000pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube is used as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Aminoacyl-tRNA synthetase complex multifunctional interacting protein 1, also known as AIMP1, is a protein encoded by the AIMP1 gene. This gene encodes a cytokine that may be induced by apoptosis and is also released from professional antigen-presenting cells, such as dendritic cells. Release of this cytokine sensitizes tumor-associated vasculature to tumor necrosis factor. Its precursor (pro-SCYE1) is identical to the p43 subunit, which is associated with multiple aminoacyl-tRNA synthetase complexes (mARSs). | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 31.2-2000 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.2 ★★★★★
Based on 5 reviews
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Product Reviews
★★★★★ 5
Fancy enough for a wedding, easy enough for a backyard party
Color: Gold, Color: Gold
I picked up this 300 Pack Premium Heavy Duty Gold Plastic Silverware set for a recent gathering, and honestly it worked out better than I expected. When you’re hosting a larger group, the last thing you want to worry about is running out of utensils or washing a mountain of dishes afterward, and this set definitely helped with that.
First off, the 300-piece set is really convenient. You get 100 forks, 100 spoons, and 100 knives, which made it easy to plan for about 100 guests without having to buy multiple packs. Everything arrived neatly organized in a clear storage box, which actually made it super easy to keep things tidy until party time.
The gold metallic finish is surprisingly nice. It gave the table a more polished, elegant look than standard plastic cutlery. I used them for a semi-formal dinner setup, and they blended in well with the decorations instead of looking cheap or out of place.
As for durability, they’re definitely heavier than typical disposable utensils. The forks handled pasta and salads just fine, and the knives worked well for softer foods like chicken and cake. They’re not quite the same as real metal silverware, of course, but for plastic they’re pretty sturdy and didn’t snap or bend easily.
I also appreciated that they’re BPA-free and disposable, which made cleanup incredibly simple once everything wrapped up.
The only small downside is that if you’re cutting tougher foods like steak, you might notice the knives aren’t quite strong enough for that. But for most party foods, buffets, and desserts, they work perfectly well.
Overall, this is a great option for parties, weddings, or large dinners when you want something that looks nicer than basic plastic but still want the convenience of disposable utensils. I’d definitely use them again for future events.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 7, 2026
★★★★★ 5
Amazing value, looks great - quite durable
Color: Gold
I recently hosted a large dinner party and decided to go with the 300 Pack Premium Heavy Duty Gold Plastic Silverware. Let me tell you, it was a great choice!
First off, the set comes with 100 forks, spoons, and knives, which was perfect for my guest list of 100. I love that it’s all in one package, so I didn’t have to stress about running out of cutlery during the event. The heavy-duty plastic feels surprisingly sturdy, and I didn’t have a single fork bend or break, even with the heartier dishes I served.
The gold metallic finish added such an elegant touch to the table setting. My guests complimented how fancy it looked for disposable cutlery! It really elevated the overall vibe of the dinner without the hassle of using real silverware or worrying about cleanup. After the meals, all I had to do was toss everything into the trash, which saved me a ton of time and effort.
Another thing I appreciated was the organized storage box. It kept everything neat and clean until I was ready to use it, which is a bonus if you're planning an event.
All in all, I highly recommend this set for anyone hosting gatherings. It combines functionality with a stylish appearance, making cleanup a breeze while still impressing your guests.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 4, 2026
★★★★★ 4
Slight odor, more flexible than I expected
Color: Gold, Color: Gold
These looked like they were going to be heavy duty, but they were more flexible than I expected (see video clip). They probably still will work well, but if you’re poking your fork into something stiff, expect that the fork might bend some.
There was a slight chemical odor when I opened the package, and I’m not a fan of that. As someone with MCS, I have to avoid exposure to fragrances and other harmful chemicals, and it makes me wonder what these are coated with. The gold might be cute for the aesthetic you’re going for with a particular gathering or party, but I find it unnecessary.
I would rather go with the real heavy duty ones that are clear plastic from a club warehouse rather than these gold ones. I feel that these are overpriced for the quality, but not a bad price if all you’re going for is the gold aesthetic.
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Reviewed in the United States on March 6, 2026
★★★★★ 5
Nice, high end look, disposable
Color: Gold, Color: Gold
I hate to spend a lot of money on something that's just going to be thrown away, so typically buy dollar store disposable silverware for parties and gatherings. But every once in a while, you want something more upscale or high end looking. Enter this pricy but pretty plastic silverware. It is perfect for a wedding, anniversary, shower or other event where you want a nicer look instead of basic.
It is sturdy. You won't have to worry about it breaking as someone spears a bite of chicken. It looks great! There's only so much you can do with plastic ware but for what it is, it looks really nice.
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Reviewed in the United States on March 24, 2026
★★★★★ 5
Great value and great biodegradable plates for snacks or lods
Size: 6", Size: 6"
These plates were exactly what I was looking for! These 6" plates are smaller ones, so if you need larger ones, then get the 8" ones:)
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 17, 2026
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