Pay in installments of $51.59 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 30 - Sep 4
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Mouse REG3g ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 600pg/mL). Then dilute to the following concentrations: 600pg/mL, 300pg/mL, 150pg/mL, 75pg/mL, 37.5pg/mL, 18.75pg/mL, 9.375pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 600pg/mL standard working solution into the first EP tube and mix thoroughly to make a 300pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Regenerating Islet-Derived Protein 3 Gamma (REG3g). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Regenerating Islet-Derived Protein 3 Gamma (REG3g) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Regenerating Islet Derived Protein 3 Gamma | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Reg3B (regenerating islet-derived protein III beta, also known as PAP-I and HIP) is a secreted 17 kDa member of the Reg gene family (C-type lectin gene superfamily). It is found in pancreatic spicule cells, intestinal enterocytes, and developing spinal motor neurons. Reg3B may play a role in intestinal epithelial cell renewal. Mouse Reg3B contains a C-type lectin domain (aa 38-173). Mature mouse Reg3B shares 59%, 71%, and 51% aa identity with the products of the other three Reg3 denominated genes (Reg III alpha, gamma, and delta, respectively). Mature mouse Reg3B also shares 91% and 67% aa identity with rat and human Reg3B, respectively. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 9.4-600 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.7 ★★★★★
Based on 15 reviews
Sort
Product Reviews
★★★★★ 5
Breathable, comfortable, warm
Color: Burgundy
Breathable during heavy exercise. Long enough to double up when used as a neck gaiter. Soft and comfortable. Warm.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 10, 2026
★★★★★ 4
Ehh
Color: Black, Color: Black
Eh. I mean, it was warm… but it trapped moisture from me breathing into it, so then it was like wet… which wasn’t ideal. Didn’t stay up on my nose super well. I had to tuck it into my goggles. Easy to wash. Decently soft. Feel like it wasn’t exactly worth the money I spent, but I know it is real wool, so that comes with a price tag. I don’t know…I really didn’t like the water / moisture getting trapped around my mouth and chin and what not. Just causing skin breakout issues. Helped cut the cold wind, but I feel like it could be better, or like there are better options out there maybe. Maybe not meant for snowboarding or exercising, so you’re not breathing as hard into it. Maybe it’s just meant for walking around with it around your neck. I don’t know.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 27, 2026
★★★★★ 5
Not very itchy, easy to put in your pocket and warm
Color: Black
I wore turtle fur for a long time and liked it except that it's too big to fit in my pocket. This wool product has really impressed me so far. It doesn't have a zero itch rating but it is very soft. It's surprisingly warm for its weight. Fits easily in my coat pockets. It's comfortable, fits well on both my neck and face as it has some stretch to it. It had a smell when I first opened the package but that went away after a couple of days. I wore it around my neck in 20F and was warm. I would say that using it as a face mask at that temperature would be pushing its limits. I find it to be a good balance between size and warmth for the Kentucky tri-state area in a mild winter. Because of it's size you can easily put a layer over it during extreme cold and it would be well worth the space it takes in your pocket
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 12, 2024
★★★★★ 5
Robbery
Color: Black
Very warm and light
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 8, 2026
★★★★★ 3
Too Short
Color: Burgundy
I like the material and construction; it's soft and thick. It is warm and is effective at temperatures in the teens and winds around 30 mph. The thickness of the material helps prevent my breath from instantly condensing and moistening the fabric. I never got the "wet dog" smell wet wool is known for. Its fit is a little tight but not prohibitively so. The color matches that of the beanie. It's too sort to be considered versatile. In wind I had to choose between shielding my neck or my face. I HIGHLY recommend you not machine wash and dry. Hand wash it and let it dry naturally.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 27, 2026
recommand products
Xtune K&N 2.75 Inch Rubber Filter Universal IN-AF-KN-RU4450 - 9932380
90.48
xTune Chevy GMC C10 / Ck Series Sierra Silverado 88-98 LED 3rd Brake Light Smoke BKL-CCK88-LED-SM - 5072405
40.00
Energy Suspension 79-04 Ford Mustang SVO Red Rear Spring Upper & Lower Isolator Set - 4.6101R
31.19
xTune Replacement Glass Mirror CS99 / Csil03 / Csil07 / Ff15097 Left Large MIR-GLASS-CS9907-MA-L1 - 9934070
23.92
Xtune Honda Del Sol 93-97 Corner Lights Amber CCL-HD93-AM - 5016478
69.68