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Description
Mouse IL19 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 500pg/mL). Then dilute to the following concentrations: 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.625pg/mL, 7.8125pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 500pg/mL standard working solution into the first EP tube and mix thoroughly to make a 250pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 19 (IL19) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Interleukin 19 (IL19) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Interleukin 19 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Interleukin-19 (IL-19) is a protein that belongs to the IL-10 cytokine subfamily. IL-19 is encoded by the IL-19 gene located on chromosome 1. The IL-19 gene consists of seven exons. The secreted IL-19 protein is composed of an α-helical structure consisting of 159 amino acids. IL-19 is an immunosuppressive cytokine responsible for downregulating cell-type-specific and system-specific inflammation. It interacts with both immune cells (macrophages, T cells, B cells) and non-immune cells (endothelial cells and brain-resident glial cells, among others). | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 7.8-500 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.9 ★★★★★
Based on 17 reviews
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Product Reviews
★★★★★ 1
Not worth the money
I've been using now for 3 weeks. I see absolutely no change in anything. No brightness, no hydration no rank or reduction.This doesn't work television forest on the hills of that.I don't work on anything. I will continue using can we change my review if I see any results.
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Reviewed in the United States on June 22, 2025
★★★★★ 5
Gentle serum with nice ingredients
This serum is a milky white color that is a mediun texture (not watery, and not super thick) and spreads well and easily. It absorbs pretty quickly, and doesn't leave me too sticky after, nor has it caused any irritation in over a week of daily use. I also haven't noticed any difference yet in terms of brightening or wrinkle reduction, but 1) know that this can take up to 12 weeks with astaxanthin, and 2) already use niacinamide and vitamin c and have probably already seen as much progress as I can expect in the way of evening out skintone and reducing the appearance of freckles.
In English, it says there are only 6 ingredients, but given the avaialable concentrations on the box (4.3% niacinamide, 0.01% astaxanthin) that doesn't make sense. The full ingredients list is only in Korean, but after using an online image translator can see that both lists include great ingredients that have all proven to be quite effective and are gentle for sensitive skin. I also really appreciate that it has the percent niacinamide on the box because this is a staple ingredient for me and so many products have it that it's nice to be able to know how much I'm using when I combine differemt serums and moisturizers that include it so that I'm in the zone that's best for my skin and not using too much.
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Reviewed in the United States on May 28, 2024
★★★★★ 4
lightweight, did not irritate my skin
I have been using this ampoule for about 3 weeks now and I like how it makes my skin feel. It doesn't feel harsh, my skin didn't seem to mind it because it didn't look or feel irritated after the 3 weeks of use. I am not solely using this product as my night time moisturizer but it is an addition to my other moisturizer. It is easy to apply, doesn't feel heavy, sticky, or tacky. I can't say how this works for existing blemishes and imperfections because I am mainly using this as a prevention. I recently had some of my sun spots laser-ed so I am trying to keep any additional sun spots from appearing again, which is why it is so important for me to keep up with the prevention routine. This product is excellent for that. I did take a look at the ingredients list and while I'm fine with most of it, I did notice there is a silicone in this. I try to avoid silicones but I'm not going to say it is a dealbreaker. All in all I like this product and will keep using this until I'm done with the entire bottle.
I didn't see the ingredients list posted on this page yet so here it is, in case you need to see what's in this serum before making the decision to purchase.
Ingredients:
Water, Butylene Glycol, Phenyl Trimethicone, Niacinamide, Tranexamic Acid, 1,2-Hexanediol, Caprylic/Capric Triglyceride, Polyglyceryl-6 Dicaprate, Arginine, Carbomer, Ethylhexylglycerin, Adenosine, Astaxanthin(100000ppb), Melia Azadirachta Flower Extract, Melia Azadirachta Leaf Extract, Betaine, Ocimum Sanctum Leaf Extract, Corallina Officinalis Extract, Coccinia Indica Fruit Extract, Curcuma Longa (Turmeric)Root Extract, Ascorbic Acid, Tocopherol, Thioctic Acid, Thermus Thermophillus Ferment, Glycerin, Potassium Sorbate
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Reviewed in the United States on May 29, 2024
★★★★★ 5
So far, loving this! I have super sensitive skin and it's causing no issues!
This serum is really nice and easy to apply! It’s on the thin side, so I like to tilt my head upwards and gently release a drop or 2 at a time onto my face. Rub it in from there.
If you drop this into your hand to apply first, you’ll be wasting a lot of product, so don’t go that route.
I do have some sun spots I’m looking to tackle, so hopefully this will help with those!
I also live in a city with high levels of pollution, so I can use all the antioxidants I can get!
I'm excited to see if this helps with some sun damage and spots that I've accumulated over the years.
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Reviewed in the United States on April 30, 2024
★★★★★ 5
Absorbs quickly
I like this ampoule. It has a nice gel-like viscosity and absorbs quickly into my skin and has not caused any irritation. I like that it has no smell. Since I have been using it, I have noticed a general lightening of the dark spots on my cheeks. I have noticed that my skin tone looks brighter since I have started using this. I will continue to use this ampoule in hopes of seeing even more improvement. Overall, I am very happy with this ampoule and think it does a good job of evening out my skin tone and lightening dark spots I have on my face making my skin look brighter, in general.
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Reviewed in the United States on July 17, 2024