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Description
Human TKTL1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against transketolase-like protein 1 (TKTL1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of transketolase-like protein 1 (TKTL1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Transketolase Like Protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Transketolase-like protein 1 (TKTL1), also known as transketolase 2 (TK2), is an enzyme encoded by the TKTL1 gene. The protein encoded by this gene is a transketolase that acts as a homodimer and catalyzes the conversion of chromobiose 7-phosphate and D-glyceraldehyde 3-phosphate to D-ribose 5-phosphate and D-xylulose 5-phosphate. This reaction links the pentose phosphate pathway with the glycolysis pathway. It catalyzes the transfer of a dicarbon ketol group from a ketose donor to an aldose acceptor via a covalent intermediate with the cofactor thiamine pyrophosphate. Mutations in this gene may be causative of Wernicke-Korsakoff syndrome. Three transcript variants encoding different isoforms have been identified for this gene. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.5 ★★★★★
Based on 26 reviews
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Product Reviews
★★★★★ 3
Prevents me from adjusting the air vent up or down
It's true that it doesn't block the air flow. I had to put it on the left passenger side vent to retain the ability to point the driver's air vents where I want. With it over there, I have to turn my head too far to the right when I'm driving to see my phone, and the passenger can't adjust the vent up or down. Also, phone can't tilt as far left and right as other ones. Good price, unique design, easy to install, feels sturdy, holds iPhone SE fine, and doesn't block my view of the entertainment system.
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Reviewed in the United States on March 27, 2026
★★★★★ 5
BETTER THAN A CUP HOLDER
I had a cup-holder mount and it was always falling apart. No matter how it was turned, to unfasten my phone I had to move the spring part of the holder in the same direction that removes the entire clip from the stem of the mount. Of course, I eventually lost the clip. What a bad idea. And, in a 4-Runner, on my cupholder, MAPS sometines got lost and I would have to hold my phone up to the windshield for a minute to get back MAPS back on track. Also not a good Idea. We're not supposed to be handeling a phone and driving so holding my phone up to the windshield is kinda like flagging down a cop. (The 4-Runner has hand-free phone service, as well as some kind of circuitious navigation but it's always lost.) . So, for about $9 on sale, I though I would try one of these LISEN mounts. It works great ! Really. Easy to attach securely but scratchlessly it to my center AC/heat vent and It is infantly moveable. Swing the arm 360 degrees, in and out, phone up or saideways, and tilt the phone to me or the Boss. If I get bored, I can relocate it anywhere along the center vents, or even over to my side vent if I have nothing better to do.. It is sturdy, quiet, easy to clamp in my S21, and release is simplye by pushing the button on the back. Sooo much better that my previous mount, for a third of the price. It might be the best $9 you ever spent. And still worthwhile,if you have to shell out the $18. (But I always try to avoid paying full price for anything). Enjoy !
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Reviewed in the United States on December 10, 2023
★★★★★ 5
Usable but average, with only basic build quality and stability.
This in-car phone mount is fairly average overall. It’s easy to install and works for basic navigation needs, but the build quality isn’t particularly impressive. The materials feel a bit ordinary, and stability can be an issue on rough roads, with some looseness over time. It’s usable, but not outstanding.
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Reviewed in the United States on February 10, 2026
★★★★★ 5
WORTH IT!!
Size: 16 Fl Oz (Pack of 1), Style: Fresh
After debating for a few weeks, I finally bought this interior cleaner hoping it would be better than the other stuff I had been using. I just got a new vehicle and really want to keep it detailed and nice. This stuff is FANTASTIC!! It made the whole cleaning process easier. I am a mom to 6 kids and between everyday school trips and busy sports seasons, the dirt and wear can add up QUICK. I needed something worth the money and to make my cleaning process easier and worth my time in this very busy season of life. It leaves a nice finish without any streaks or oily appearance. It dried in a timely manner and my Suburban looks and smells wonderful.
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Reviewed in the United States on May 3, 2026
★★★★★ 5
Nice Interior Cleaner / No Residue and No Shine
Size: 16 Fl Oz (Pack of 1), Style: Fresh
Most people knock Chemical Guys and it appears a rather basic , not boutique, car care line. This is one hit in the line and actually comes in at least three versions based upon the scent left after use. This one is a baby powder scent that does not linger too long nor to heavy.
The cleaning power is light with no streaks. It dries quickly and does not appear to leave any markings. A light spritz on doors, dash, and interior parts and a quick wipe with MF. You could use a dampen towel also. It leaves behind no shine or dressing afterwards which is wonderful for those wanting a fresh like new appearance without the shine or goopy sticky mess.
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Reviewed in the United States on May 25, 2026
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